CBL1304 | Anti-Integrin α4 Antibody, clone PS/2

CBL1304
500 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MFC, IF, IP, IHC, FUNCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL1304
      Replaces04-1131
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin α4 Antibody, clone PS/2
      Alternate Names
      • CD49d
      • VLA-4
      Background InformationVirtually all lymphoid and myeloid cells in bone marrow are reactive with the anti-VLA-4 antibody. VLA-4 is absent or minimally detectable on stromal cells. Myeloid cells maintained in Dexter-type long-term cultures of murine bone marrow are positive, as are lymphoid cells from Whitlock-White cultures.
      References
      Product Information
      FormatPurified
      PresentationPurified rat IgG2bκ in 0.01 M phosphate buffer, pH 7.1 containing no preservatives.
      Applications
      ApplicationAnti-Integrin α4 Antibody, clone PS/2 detects level of Integrin α4 & has been published & validated for use in FC, IF, IP, IH.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Immunohistochemistry
      • Affects Function
      Application NotesFlow cytometry: 2 μg per 106 cells

      Immunohistochemistry on frozen tissue sections

      Immunoprecipitation

      Cell adhesion assays

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ClonePS/2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityClone PS/2 recognises the murine alpha4 chain of the VLA-4 integrin heterodimer, 150 kDa. The antibody also reacts with human VLA-4.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain.
      Gene Symbol
      • ITGA4
      • IA4
      • MGC90518
      • VLA-4
      • CD49D
      • CD49d
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13612 # Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
      SIZE: 1038 amino acids; 115333 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P13612 The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
      PTM: Phosphorylation on Ser-1027 inhibits PXN binding.
      SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20ºC for up to 12 months from date of receipt.Upon first use, aliquot into smaller quantities to avoid additional freeze/thaw cycles.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Integrin α4 Antibody, clone PS/2 -2769898 2769898
      Anti-Integrin α4 Antibody, clone PS/2 -2829802 2829802
      Anti-Integrin 4 Antibody, clone PS/2 -2783101 2783101
      RAT ANTI-MOUSE INTEGRIN alpha4 [CD49d] MONOCLONAL ANTIBODY - 2115974 2115974
      RAT ANTI-MOUSE INTEGRIN alpha4 [CD49d] MONOCLONAL ANTIBODY - 2391072 2391072
      RAT ANTI-MOUSE INTEGRIN alpha4 [CD49d] MONOCLONAL ANTIBODY - 2430533 2430533
      RAT ANTI-MOUSE INTEGRIN alpha4 [CD49d] - 2494039 2494039
      RAT ANTI-MOUSE INTEGRIN alpha4 [CD49d] -2710233 2710233
      RAT ANTI-MOUSE INTEGRIN alpha4 [CD49d] -2717654 2717654
      RAT ANTI-MOUSE INTEGRIN alpha4 [CD49d] -2740943 2740943

      References

      Reference overviewPub Med ID
      Integrins and cAMP mediate netrin-induced growth cone collapse.
      Lemons, ML; Abanto, ML; Dambrouskas, N; Clements, CC; Deloughery, Z; Garozzo, J; Condic, ML
      Brain research  1537  46-58  2013

      Show Abstract
      24001590 24001590
      Integrin alpha4beta1-VCAM-1-mediated adhesion between endothelial and mural cells is required for blood vessel maturation
      Garmy-Susini, B. et al.
      J. Clin. Invest., 115:1542-1551 (2005)  2005

      15902308 15902308

      Data Sheet

      Title
      Anti-Integrin alpha4, clone PS/2 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies