CBL1512Z | Anti-Integrin αM [CD11b] Antibody, clone OX-42

CBL1512Z
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RFC, IP, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL1512Z
      ReplacesMAB1405Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin αM [CD11b] Antibody, clone OX-42
      Alternate Names
      • CD11b
      References
      Product Information
      FormatPurified
      PresentationThe immunoglobulin was purified by protein G affinity chromatography and is presented as a liquid in phosphate buffered saline containing no preservatives.
      Applications
      ApplicationDetect Integrin αM [CD11b] using this Anti-Integrin αM CD11b Antibody, clone OX-42 validated for use in FC, IH, IH(P) & IP.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      Application NotesFlow cytometry

      Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15' no post-fix).

      Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG bead for capture only. The use of protein A or protein G is not recommended.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRat peritoneal macrophages
      CloneOX-42
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes most macrophages (including resident peritoneal and activated macrophages), Kupffer cells, but only about 35% of alveolar macrophages. The antibody also labels dendritic cells extensively, granulocytes and cells with the morphology of microglia in brain. The antibody precipitates three polypeptides of 150, 103 and 95 KDa. The antibody inhibits complement mediated rosettes and is probably the rat equivalent of the human receptor for IC3b called CR3. FUSION PARTNER: NSO myeloma cell line.
      IsotypeIgG2a
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Some integrin alpha M proteins contain an inserted amino acid (Q, between AA positions 499 and 500) resulting from a 3bp shift in a splice acceptor that inserts the codon CAG; receptors with or without the extra amino acid are functionally indistinguishable
      Gene Symbol
      • ITGAM
      • MO1A
      • CD11b
      • MAC1A
      • CD11B
      • MAC-1
      • CR3A
      • MGC117044
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11215 # Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
      SIZE: 1152 amino acids; 127179 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Predominantly expressed in monocytes and granulocytes.
      DOMAIN: SwissProt: P11215 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
      SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain in undiluted aliquots at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-RAT INTEGRIN alphaM [CD11b] MONOCLONAL ANTIBODY - 2109509 2109509
      MOUSE ANTI-RAT INTEGRIN alphaM [CD11b] -2779495 2779495
      MOUSE ANTI-RAT INTEGRIN alphaM [CD11b] MONOCLONAL ANTIBODY - 2044292 2044292
      MOUSE ANTI-RAT INTEGRIN alphaM [CD11b] MONOCLONAL ANTIBODY - 2064706 2064706
      MOUSE ANTI-RAT INTEGRIN alphaM [CD11b] MONOCLONAL ANTIBODY - 2070403 2070403
      MOUSE ANTI-RAT INTEGRIN alphaM [CD11b] MONOCLONAL ANTIBODY - 2281666 2281666
      MOUSE ANTI-RAT INTEGRIN alphaM [CD11b] MONOCLONAL ANTIBODY - 2322530 2322530

      References

      Reference overviewPub Med ID
      Hypertonic saline alleviates cerebral edema by inhibiting microglia-derived TNF-α and IL-1β-induced Na-K-Cl Cotransporter up-regulation.
      Huang, LQ; Zhu, GF; Deng, YY; Jiang, WQ; Fang, M; Chen, CB; Cao, W; Wen, MY; Han, YL; Zeng, HK
      Journal of neuroinflammation  11  102  2014

      Show Abstract
      24916922 24916922
      Impact of vegf on astrocytes: analysis of gap junctional intercellular communication, proliferation, and motility.
      Ricarda Wuestefeld,Jingchen Chen,Karl Meller,Beate Brand-Saberi,Carsten Theiss
      Glia  60  2012

      Show Abstract
      22431192 22431192
      Stainless steel ions stimulate increased thrombospondin-1-dependent TGF-beta activation by vascular smooth muscle cells: implications for in-stent restenosis.
      Pallero, MA; Talbert Roden, M; Chen, YF; Anderson, PG; Lemons, J; Brott, BC; Murphy-Ullrich, JE
      Journal of vascular research  47  309-22  2010

      Show Abstract Full Text Article
      20016205 20016205
      Erythropoietin receptor expression is concordant with erythropoietin but not with common beta chain expression in the rat brain throughout the life span.
      Pascal E Sanchez,Fabrice P Navarro,Raafat P Fares,Jérémie Nadam,Béatrice Georges,Colette Moulin,Marion Le Cavorsin,Chantal Bonnet,Philippe Ryvlin,Amor Belmeguenai,Jacques Bodennec,Anne Morales,Laurent Bezin
      The Journal of comparative neurology  514  2009

      Show Abstract
      19330822 19330822
      A protocol for phenotypic detection and characterization of vascular cells of different origins in a lung neovascularization model in rodents.
      Jones, RC; Capen, DE; Cohen, KS; Munn, LL; Jain, RK; Duda, DG
      Nature protocols  3  388-97  2008

      Show Abstract
      18323810 18323810
      Neuroprotective effects of erythropoietin in the rat hippocampus after pilocarpine-induced status epilepticus.
      Jérémie Nadam, Fabrice Navarro, Pascal Sanchez, Colette Moulin, Béatrice Georges, Aël Laglaine, Jean-Marc Pequignot, Anne Morales, Philippe Ryvlin, Laurent Bezin
      Neurobiology of disease  25  412-26  2007

      Show Abstract
      17166730 17166730
      Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
      Whiteland, J L, et al.
      J. Histochem. Cytochem., 43: 313-20 (1995)  1995

      Show Abstract
      7868861 7868861
      Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain
      Milligan C.E. et al.
      J Comparative Neurol., 314:125-135 (1991)  1991

      1797868 1797868
      Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3
      Robinson A.P. et al.
      Immunol., 57:239-247 (1986)  1986

      3512425 3512425

      Data Sheet

      Title
      MOUSE ANTI-RAT INTEGRIN alphaM [CD11b]

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies