06-1125 | Anti-Ipaf (NLRC4) Antibody

06-1125
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1125
      DescriptionAnti-Ipaf (NLRC4) Antibody
      Alternate Names
      • NLR family, CARD domain containing 4
      • NOD-like receptor C4
      • caspase recruitment domain family, member 12
      • ICE-protease activating factor
      • nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4
      • NLR family CARD domain-containing protein 4
      • Caspase recruitment domain-containing protein 12
      • Ice protease-activating factor
      • Clan protein
      • CARD, LRR, and NACHT-containing protein
      Background InformationIpaf, also known as NLRC4, is a human CED-4 homologue and is thought to be an activator of pro-caspase-1 and might also be associated with the activation of caspase-1 as a response to pro-inflammatory and apoptotic stimulus. It has been shown to be induced by p53 and contributes to p53-dependent apoptosis. Ipaf is thought to play a role in the activation of the innate immune system.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human lung tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Ipaf (NLRC4) Antibody is validated for use in IC, WB for the detection of Ipaf (NLRC4).
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blot Analysis: 1 ug/ml of this antibody detected Ipaf in 10 ug of human lung tissue lysate.

      Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Ipaf in NIH/3T3 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Ipaf (NLRC4) at the LRR4 domain.
      EpitopeLRR4 domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Ipaf at the LRR4 domain.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySummary: In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM].
      Gene Symbol
      • IPAF
      • CLAN
      • CARD12
      • CLAN1
      • ipaf
      • CLR2.1
      • CLAND
      • CLANA
      • CLANB
      • CLANC
      • Ipaf
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Plays a role in the promotion of apoptosis.

      SUBUNIT STRUCTURE: Self-associates and binds to ASC, pro-caspase-1, NOD2, BCL10 and NALP1 (NAC) by CARD-CARD interaction.

      SUBCELLULAR LOCATION: Cytoplasm. Note: Cytoplasmic filaments.

      TISSUE SPECIFICITY: Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.

      SEQUENCE SIMILARITIES: Contains 1 CARD domain.

      Contains 14 LRR (leucine-rich) repeats.

      Contains 1 NACHT domain.
      Molecular Weight~112 kDa observed.
      Different lysates may detect different isoforms and splice variants (Isoform 1: 112 kDa, Isoform 2: 41 kDa, Isoform 3: 18 kDa, Isoform 4: 10 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human lung tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Ipaf in 10 µg of human lung tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Ipaf (NLRC4) - 2460973 2460973
      Anti-Ipaf (NLRC4) - 2006955 2006955
      Anti-Ipaf (NLRC4) - 2207244 2207244
      Anti-Ipaf (NLRC4) - 2360016 2360016
      Anti-Ipaf (NLRC4) - 2901568 2901568
      Anti-Ipaf (NLRC4) - 2930214 2930214
      Anti-Ipaf (NLRC4) - NG1951079 NG1951079
      Anti-Ipaf (NLRC4) - NRG1855876 NRG1855876
      Anti-Ipaf (NLRC4) -2505429 2505429
      Anti-Ipaf (NLRC4) -2610516 2610516

      References

      Reference overviewApplicationPub Med ID
      An activating NLRC4 inflammasome mutation causes autoinflammation with recurrent macrophage activation syndrome.
      Canna, SW; de Jesus, AA; Gouni, S; Brooks, SR; Marrero, B; Liu, Y; DiMattia, MA; Zaal, KJ; Sanchez, GA; Kim, H; Chapelle, D; Plass, N; Huang, Y; Villarino, AV; Biancotto, A; Fleisher, TA; Duncan, JA; O'Shea, JJ; Benseler, S; Grom, A; Deng, Z; Laxer, RM; Goldbach-Mansky, R
      Nature genetics  46  1140-6  2014

      Show Abstract
      Western Blotting25217959 25217959