06-1310 | Anti-JAK2 Antibody

06-1310
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, R, MWB, IPRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1310
      DescriptionAnti-JAK2 Antibody
      Alternate Names
      • Janus kinase 2
      • Janus kinase 2 (a protein tyrosine kinase)
      • tyrosine-protein kinase JAK2
      Background InformationThe Janus family of tyrosine kinases (JAK) includes JAK1, JAK2, JAK3 and Tyk2. This family associates with a variety of cytokine receptors and is activated by ligand binding. Upon ligand binding to cytokine receptors, JAKs phosphorylate themselves and their associated receptors. This provides multiple binding sites for signaling proteins containing SH2 or other phospho-tyrosine-binding domains, including STATs, Shc, insulin receptor substrates and FAK.
      References
      Product Information
      FormatPurified
      Control
      • RAW264.7 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-JAK2 Antibody is validated for use in WB, IP for the detection of JAK2.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blot Analysis: 1 µg/mL from a previous lot detected JAK2 in 10 µg of L6 cell lysate.

      Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JAK2 from RAW264.7 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the protein kinase 1 domain of mouse JAK2.
      EpitopeProtein kinase 1 domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the protein kinase 1 domain of JAK2.
      Species Reactivity
      • Mouse
      • Human
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • JAK2
      • JAK-2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Tyrosine kinase of the non-receptor type, involved in interleukin-3 and probably interleukin-23 signal transduction (By similarity). Plays a role in leptin signaling and control of body weight.

      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

      SUBUNIT STRUCTURE: Interacts with IL23R, SKB1 and STAM2 (By similarity). Interacts with SIRPA and SH2B1.

      SUBCELLULAR LOCATION: Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated (By similarity).

      DOMAIN: Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.

      PTM: Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

      Contains 1 FERM domain.

      Contains 1 protein kinase domain.

      Contains 1 SH2 domain.
      Molecular Weight~130 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in RAW264.7 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected JAK2 in 10 µg of RAW264.7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-JAK2 - 2459501 2459501
      Anti-JAK2 - 1976933 1976933
      Anti-JAK2 - 2030041 2030041
      Anti-JAK2 - 2227187 2227187
      Anti-JAK2 - JBC1794875 JBC1794875
      Anti-JAK2 - NG1842853 NG1842853
      Anti-JAK2 - NG1888896 NG1888896
      Anti-JAK2 - NG1928926 NG1928926
      Anti-JAK2 - NRG1732250 NRG1732250
      Anti-JAK2 -2512445 2512445

      References

      Reference overviewPub Med ID
      Multiple oligomerization domains of KANK1-PDGFRβ are required for JAK2-independent hematopoietic cell proliferation and signaling via STAT5 and ERK.
      Medves, S; Noël, LA; Montano-Almendras, CP; Albu, RI; Schoemans, H; Constantinescu, SN; Demoulin, JB
      Haematologica  96  1406-14  2011

      Show Abstract
      21685469 21685469

      Brochure

      Title
      Pathways and Biomarkers of JAK/STAT Signaling

      Technical Info

      Title
      JAK/STAT Signaling Research Focus

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies