07-1487 | Anti-JAK3 Antibody

07-1487
100 µL  
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      Overview

      Replacement Information
      Replacement Information07-1487 is a recommended replacement for AB3806.

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IPRbSerumPolyclonal Antibody
      Description
      Catalogue Number07-1487
      ReplacesAB3806
      DescriptionAnti-JAK3 Antibody
      Alternate Names
      • Janus Kinase 3
      • Janus kinase 3 (a protein tyrosine kinase, leukocyte)
      • Leukocyte janus kinase
      • Tyrosine-protein kinase JAK3
      Background InformationJAKs are non-receptor tyrosine kinases which couple to cytokine receptors, and are activated by cytokines. JAK kinases (JAK1, JAK2, JAK3, Tyk2) have a kinase domain and a second, pseudokinase domain which is not enzymatically active. The principal effectors of JAKs are the STATs, which are direct activators of transcription, but JAKs also signal to the Ras-MAP Kinase pathway, and through Tec, to the PI3 Kinase pathway. JAK3 is required for signaling through cytokine receptors which share a common γ chain. JAK2 is required for signaling through a different subset of cytokine receptors, while JAK1 is essential for signaling from the IL-2, IL-6 and interferon receptors. The stress-activated protein kinase 1 (SAPK) family is also referred to as the jun N-terminal kinase family in light of the substrate preference of these serine/threonine kinases. In addition to regulating many biological processes including proliferation and apoptosis, the JNK/SAPK family has been implicated in many neurodegenerative diseases including Alzheimer disease, Parkinson's disease and Amyotrophic lateral sclerosis.
      References
      Product Information
      FormatSerum
      Control
      • HeLa cell lysate
      PresentationRabbit polyclonal IgG whole antiserum in buffer containing sodium azide.
      Applications
      ApplicationDetect JAK3 using this Anti-JAK3 Antibody validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation: 5-10 uL of a previous lot of this antibody was used in immunoprecipitation.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 782-797 of mouse JAK3 (SDPTPGIPSPRDELC).
      HostRabbit
      SpecificityRecognizes JAK3 (Janus Kinase 3).
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryJAK3 encodes Janus kinase 3, a tyrosine kinase that belongs to the Janus family. JAK3 functions in signal transduction and interacts with members of the STAT (signal transduction and activators of transcription) family. JAK3 is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations that abrogate Janus kinase 3 function cause an autosomal SCID (severe combined immunodeficiency disease).
      Gene Symbol
      • JAK3
      • JAKL
      • LJAK
      • JAK3_HUMAN
      • L-JAK
      • JAK-3
      • EC 2.7.10.2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P52333 # Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
      SIZE: 1124 amino acids; 125099 Da
      SUBUNIT: Interacts with STAM2 and MYO18A (By similarity). Interacts with SHB.
      SUBCELLULAR LOCATION: Intracytoplasmic membrane; Peripheral membrane protein (By similarity). Note=Wholly intracellular, possibly membrane associated (By similarity).
      TISSUE SPECIFICITY: In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B- and M-forms are detected in cells both of hematopoietic and epithelial origins.
      DOMAIN: SwissProt: P52333 Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
      PTM: Tyrosine phosphorylated in response to IL-2 and IL-4.
      DISEASE: SwissProt: P52333 # Defects in JAK3 are a cause of autosomal recessive T-cell negative/B-cell positive type of severe combined immunodeficiency (SCID) [MIM:600802]; a condition characterized by the absence of circulating mature T-lymphocytes and NK cells, normal to elevated numbers of nonfunctional B-lymphocytes, and marked hypoplasia of lymphoid tissues.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain. & Contains 1 SH2 domain
      Molecular Weight125 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on HeLa lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected JAK3 on 10 μg of HeLa lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies