07-1533 | Anti-JMJD3 (NT) Antibody

07-1533
400 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIHC, ICC, ELISA, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1533
      DescriptionAnti-JMJD3 (NT) Antibody
      Alternate Names
      • JMJD
      • JmjC domain-containing protein 3
      • Jumonji domain-containing protein 3
      • Lysine demethylase 6B
      • jumonji domain containing 3
      • jumonji domain containing 3
      • histone lysine demethylase
      Background InformationJMJD3 (Jumonji domain-containing protein 3) is part of the family of novel JmjC domain-containing histone demethylation (JHDM) enzymes. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. JMJD3 specifically demethylates Lys27 on histone H3, demethylating both the trimethylated and dimethylated form. JMJD3 plays a role in the regulation of posterior development by regulating HOX gene expression. It is also involved in the inflammatory response by regulating gene expression and macrophage differentiation
      References
      Product Information
      FormatPurified
      Control
      • HeLa cells
      PresentationPurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
      Applications
      ApplicationUse Anti-JMJD3 (N-terminus) Antibody (Rabbit Polyclonal Antibody) validated in IHC, ICC, ELISA, WB to detect JMJD3 (N-terminus) also known as JmjC domain-containing protein 3.
      Key Applications
      • Immunohistochemistry
      • Immunocytochemistry
      • ELISA
      • Western Blotting
      Application NotesELISA:
      This antibody is reported by an
      outside laboratory to be suitable for ELISA.

      Immunohistochemistry:
      This antibody is reported by an outside laboratory to be suitable for immuno-histochemistry.

      Immunocytochemistry Analysis:
      Confocal immunofluorescence analysis of HeLa and NIH/3T3 using 1:100 dilution of anti-JMJD3 (N-Terminus) (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green).

      Western Blot Analysis:
      The antibody recognizes JMJD3 in HeLa cell lysates.
      Biological Information
      ImmunogenThis antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human JMJD3.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes the N-terminal region of human JMJD3. Additional lower MW bands are detected in some lysates that may be degradation products.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman and mouse. Not tested in other species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EC 1.14.11
      • KDM6B
      • KIAA0346
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Ref.5 Ref.6

      Cofactor Ascorbate By similarity.

      Fe(2+). Ref.5

      SUBUNIT STRUCTURE: Interacts with TLE1 By similarity. Component of the MLL4 complex, at least composed of MLL4, ASH2L, RBBP5, WDR5, and JMJD3.

      SUBCELLULAR lOCATION: NucleusProbable.

      INDUCTION: By 12-O-tetradecanoylphorbol-13-acetate (TPA) in myeloid leukemia cells. Ref.4

      SIMILARITY: Belongs to the UTX family.

      Contains 1 JmjC domain.

      Molecular Weight~180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceImmunocytochemisty:
      Recognizes JMJD3 in HeLa, NIH 3T3, and A431 cells by cytochemistry (1:50 – 1:100 dilution).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size400 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-JMJD3 (N-terminus) - 2398823 2398823
      Anti-JMJD3 (N-terminus) - 2459015 2459015
      Anti-JMJD3 (N-terminus) - NG1809719 NG1809719
      Anti-JMJD3 (N-terminus) - 2007353 2007353
      Anti-JMJD3 (N-terminus) - 2036694 2036694
      Anti-JMJD3 (N-terminus) - 2168272 2168272
      Anti-JMJD3 (N-terminus) - 3062789 3062789
      Anti-JMJD3 (N-terminus) - NG1593387 NG1593387
      Anti-JMJD3 (N-terminus) - NG1627562 NG1627562
      Anti-JMJD3 (N-terminus) - NG1748991 NG1748991

      References

      Reference overviewPub Med ID
      Polycomb eviction as a new distant enhancer function.
      Vernimmen, D; Lynch, MD; De Gobbi, M; Garrick, D; Sharpe, JA; Sloane-Stanley, JA; Smith, AJ; Higgs, DR
      Genes & development  25  1583-8  2011

      Show Abstract
      21828268 21828268
      Carcinoma in situ testis displays permissive chromatin modifications similar to immature foetal germ cells.
      Almstrup, K; Nielsen, JE; Mlynarska, O; Jansen, MT; Jørgensen, A; Skakkebæk, NE; Rajpert-De Meyts, E
      British journal of cancer  103  1269-76  2010

      Show Abstract
      20823885 20823885

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies