CBL218-I | Anti-Keratin K3/K76 Antibody, clone AE5

CBL218-I
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, RbWB, IHC, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL218-I
      ReplacesCBL218
      DescriptionAnti-Keratin K3/K76 Antibody, clone AE5
      Alternate Names
      • 65 kDa cytokeratin
      • Cytokeratin-3
      • CK-3
      • Keratin-3
      • K3
      • Type-II keratin Kb3
      Background InformationCytokeratin 3, also known as 65kDa Cytokeratin or Keratin K3 is a specific keratin found primarily in the corneal epithelium and minorly in other epithelium like that of the esophagus. The corneal epithelium tissue forms the outermost layer of the cornea, which is the clear front covering of the eye. Keratins are divided into two primary types based upon their isoelectric mobility and size, acidic, or type 1:40-55kDa, and basic to neutral or type 2, 56-70kDa. Cytokeratin 3 is a type II cytokeratin. Type II cytokeratins form pairs with other cytokeratins. Cytokeratin 3 is expressed in the corneal epithelium with Cytokeratin 12. Together Cytokeratin 3 and Cytokeratin 12 form molecules known as intermediate filaments. These filaments assemble into strong networks that provide strength and resilience to the corneal epithelium. Mutations in Cytokeratin 3 have been associated with Meesmann's Corneal Dystrophy a disease characterized by fragility of the anterior corneal epithelium.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Keratin K3/K76 Antibody, clone AE5 is validated for use in Western Blotting and Immunohistochemistry and Immunofluorescence for the detection of Keratin K3/K76.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Keratin K3/K76 in bovine cornea epithelial cells.
      Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in Keratin 3 expressed epithelial cells on the surface of submerged and AL explants, indicating that they were derived from limbal progenitor cells via migration. (Kawakita, T., et al. (2005) Am J Pathol, 167:381-93.).
      Western Blotting Analysis: A representative lot was used by an independent laboratory in Rabbit epithelial Keratin lysate. (Schermer, A, et al. (1986) J. Cell Biol., 103: 49-62).
      Western Blotting Analysis: A representative lot was used by an independent laboratory in rabbit corneal tissue lysate. (Cooper, D., et al. (1986) J. Biol. Chem., 261: 4646-54).
      Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in human limbal stem cells cultured in medium supplemented with human EGF. (Sharifi, A.M., et al. (2010) Biocell. 34(1):53-55).
      Immunofluorescence Analysis: A representative lot was used by an independent laboratory in rabbit corneal and limbal tissues, limbal explant, and epithelial outgrowth on human AM. (Wang D.Y., et al. (2003) Invest Ophthalmol Vis Sci. 44(11):4698-704).
      Immunofluorescence Analysis: A representative lot was used by an independent laboratory inRabbit corneal epithelial Keratin cells. (Schermer, A, et al. (1986) J. Cell Biol., 103: 49-62).
      Biological Information
      ImmunogenWhole cells corresponding to Rabbit Keratin, type II cytosleletal 3.
      CloneAE5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Bovine
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KRT3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~60 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rabbit eye (cornea) cell lysate.

      Western Blotting Analysis: 0.05 µg/mL of this antibody detected Keratin K3/K76 in 10 µg of Rabbit eye (cornea) cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Keratin K3/K76 Antibody, clone -2789709 2789709
      Anti-Keratin K3/K76 Antibody, clone AE5 Q2476917
      Anti-Keratin K3/K76 Antibody, clone AE5 -2854164 2854164
      Anti-Keratin K3/K76 Antibody, clone AE5 Monoclonal Antibody 3005277
      Anti-Keratin K3/K76 Antibody, clone AE5 Monoclonal Antibody 2918011
      Anti-Keratin K3/K76 Antibody, clone AE5, Monoclonal Antibody 2839592

      References

      Reference overviewPub Med ID
      Isolation, culture, characterization and optimization of human corneal stem cells.
      Sharifi, AM; Darabi, R; Jadidi, K
      Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al  34  53-5  2010

      Show Abstract
      20506631 20506631
      Intrastromal invasion by limbal epithelial cells is mediated by epithelial-mesenchymal transition activated by air exposure.
      Kawakita, T; Espana, EM; He, H; Li, W; Liu, CY; Tseng, SC
      The American journal of pathology  167  381-93  2005

      Show Abstract
      16049325 16049325
      Propagation and phenotypic preservation of rabbit limbal epithelial cells on amniotic membrane.
      Wang, DY; Hsueh, YJ; Yang, VC; Chen, JK
      Investigative ophthalmology & visual science  44  4698-704  2003

      Show Abstract
      14578389 14578389
      Monoclonal antibody analysis of bovine epithelial keratins. Specific pairs as defined by coexpression.
      Cooper, D; Sun, TT
      The Journal of biological chemistry  261  4646-54  1986

      Show Abstract
      2420789 2420789
      Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells.
      Schermer, A; Galvin, S; Sun, TT
      The Journal of cell biology  103  49-62  1986

      Show Abstract
      2424919 2424919