06-1002 | Anti-LAP2 Antibody

06-1002
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ca, M, RWB, ICC, IFRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1002
      DescriptionAnti-LAP2 Antibody
      Alternate Names
      • LEM domain containing 4
      • TP alpha
      • TP beta/gamma
      • TPRP isoform alpha
      • TPRP isoforms beta/gamma
      • Thymopoietin isoform alpha
      • Thymopoietin, isoforms beta/gamma
      • Thymopoietin-related peptide isoform alpha
      • Thymopoietin-related peptide isoforms beta/gamma
      • lamina-associated polypeptide 2
      Background InformationThe inner membrane of the nuclear envelope is connected to the nuclear lamina. The main components of the nuclear lamina are Type V intermediate filaments called lamins and lamin binding proteins including lamin B receptor, emerin, MANI, LAP1 (3 isoforms), and LAP2 (several isoforms). The lamins are divided into two groups: the constitutively expressed lamin B and the developmentaly regulated lamin A.
      The best characterized isoforms of lamin binding protein LAP2 are LAP2α and LAP2β. LAP2β is a type II membrane protein in the inner nuclear membrane that binds lamin B and is important to cell viability and controlling nuclear lamina growth. LAP2α has been characterized as a nucleoplasmic protein that interacts with A-type lamins to control gene expression, transcription, and chromatin organization.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.
      Applications
      ApplicationAnti-LAP2 Antibody is an antibody against LAP2 for use in WB, IC & IF.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesImmunofluorescence:
      Representative lot data. This antibody was used to detect the nuclear lamina by immunofluorescence.

      Immunocytochemistry:
      Representative lot data.
      A previous lot was used in confocal fluorescent analysis of A431, HeLa and NIH/3T3 cells using anti-LAP2 rabbit polyclonal antibody (Red).
      Actin filaments have been labeled with AlexaFluor® 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining.
      Biological Information
      ImmunogenKLH-conjugated synthetic linear peptide corresponding to LAP2.
      HostRabbit
      SpecificityThis antibody recognizes LAP2.
      Species Reactivity
      • Human
      • Canine
      • Mouse
      • Rat
      Species Reactivity NoteTested on human and mouse. Predicted to cross-react with canine (dog) and rat based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CMD1T
      • LAP2
      • LEMD4
      • MGC61508
      • PRO0868
      • TP
      • thymopoietin
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with NAKAP95.

      TP and TP5 may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.

      SUBUNIT STRUCTURE: Interacts with LMNB1, LMNB2, BANF1, NAKAP95, GMCL and chromosomes By similarity.

      SUBCELLULAR lOCATION: Nucleus inner membrane; Single-pass type II membrane protein. Note= Tightly associated with the nuclear lamina.

      TISSUE SPECIFICTY: Expressed in many tissues. Most abundant in adult thymus and fetal liver.

      DOMAIN: Has two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1.

      PTM: Mitosis-specific phosphorylation specifically abolishes its binding to lamin B and chromosomes By similarity.

      PHARMACEUTICAL USE: TP5 is available under the names Timunox (Cilag), Sintomodulina (Italofarmaco) and Mepentil (Recordati). Used in primary and secondary immune deficiencies, autoimmunity, infections and cancer.

      SIMILARITY: Belongs to the LEM family.

      Contains 1 LEM domain.

      Contains 1 LEM-like domain.

      Molecular Weight~55 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on HeLa cell lysate.

      Western Blot Analysis:
      A 1:1000-1:3000 dilution of this antibody was used to detect LAP2 in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-LAP2 2476747
      Anti-LAP2 - 1962091 1962091
      Anti-LAP2 - 2316806 2316806
      Anti-LAP2 - NG1599499 NG1599499
      Anti-LAP2 -2608444 2608444
      Anti-LAP2 -2669861 2669861
      Anti-LAP2 -2778480 2778480
      Anti-LAP2 -2794472 2794472
      Anti-LAP2 Polyclonal Antibody 2884653

      References

      Reference overviewPub Med ID
      A flow cytometry-based screen of nuclear envelope transmembrane proteins identifies NET4/Tmem53 as involved in stress-dependent cell cycle withdrawal.
      Korfali, N; Srsen, V; Waterfall, M; Batrakou, DG; Pekovic, V; Hutchison, CJ; Schirmer, EC
      PloS one  6  e18762  2011

      Show Abstract
      21533191 21533191