05-561 Anti-LAT Antibody, clone 2E9

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-561
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-LAT Antibody, clone 2E9
      Product Information
      • Positive Control Included: Jurkat cell lysate (12-303)
      PresentationProtein A Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide.
      ApplicationDetect LAT using this Anti-LAT Antibody, clone 2E9 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 31-233 of human LAT
      SpecificityRecognizes both forms of LAT.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms.
      Gene Symbol
      • LAT
      • pp36
      • LAT1
      • p36-38
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43561 # Required for TCR (T-cell antigen receptor)- and pre-TCR- mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
      SIZE: 262 amino acids; 27930 Da
      SUBUNIT: When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB and SKAP2.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type III membrane protein. Note=Present in lipid rafts.
      TISSUE SPECIFICITY: Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
      PTM: Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. & Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
      MISCELLANEOUS: Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell- induced activation of PLC, maybe by inducing the dephosphorylation of LAT.
      Molecular Weight36/38 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from Jurkat cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-LAT (mouse monoclonal IgG) - 21171622117162
      Anti-LAT (mouse monoclonal IgG) - 21422252142225
      Anti-LAT (mouse monoclonal IgG) - 24531992453199
      Anti-LAT (mouse monoclonal IgG) - 343891343891
      Anti-LAT - JBC1852281JBC1852281
      Anti-LAT - JBC1873435JBC1873435
      Anti-LAT -25157122515712
      Anti-LAT -26991902699190
      Anti-LAT -27272092727209
      Anti-LAT -27831072783107


      Reference overviewApplicationPub Med ID
      A conserved motif in the ITK PH-domain is required for phosphoinositide binding and TCR signaling but dispensable for adaptor protein interactions.
      Hirve, N; Levytskyy, RM; Rigaud, S; Guimond, DM; Zal, T; Sauer, K; Tsoukas, CD
      PloS one  7  e45158  2012

      Show Abstract
      23028816 23028816
      LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation.
      Zhang, W, et al.
      Cell, 92: 83-92 (1998)  1998

      Show Abstract
      Immunoblotting (Western)9489702 9489702

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      Included Positive Control

      Catalogue Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability


      Life Science Research > Antibodies and Assays > Primary Antibodies