05-714 | Anti-Lamin A/C Antibody, clone 14

05-714
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Ca, ChICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-714
      ReplacesMABE481
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Lamin A/C Antibody, clone 14
      Background InformationNuclear lamins are composed of the type V intermediate filament proteins. Often referred to as nucleoskeletal proteins they play a key role in nuclear integrity, positioning of nuclear pores, and overall nuclear size and shape. They also play several key functional roles in the maintenance and propagation of the genome--replication, transcription-- as well as the disassembly and reassembly of the nucleus during cell division. In vitro studies have shown that dimers are the basic building blocks of higher order lamin structures and in low concentrations lamins are distributed throughout the nucleoplasm. In humans, there are two types of lamins: A-type lamins (lamins A and C), found primarily in differentiated cells, and B-type lamins (lamins B1 and B2), found in all nucleated cells. Nuclear lamins are involved in a number of essential nuclear functions, including nuclear envelope assembly and disassembly during cell division, DNA synthesis, transcription, and apoptosis. Nuclear lamins have been found to co-localize with DNA synthesis sites.
      References
      Product Information
      FormatPurified
      Control
      • Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 50% storage buffer (20 mM sodium phosphate, pH 7.5, 0.15 M NaCl, 1 mg/mL BSA, 0.09% sodium azide) and 50% glycerol. Store at -20°C.
      Applications
      ApplicationAnti-Lamin A/C Antibody, clone 14 detects level of Lamin A/C & has been published & validated for use in IC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      This antibody has been reported by an independent laboratory to detect Lamin A/C in human endothelial cells.
      Biological Information
      ImmunogenPeptide from human lamin A/C corresponding to amino acids 398 to 490.
      Clone14
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Lamin A, MW ~74 kDa and Lamin C, MW ~65 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Canine
      • Chicken
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Through alternate splicing, this gene encodes three type A lamin isoforms. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. [provided by RefSeq].
      Gene Symbol
      • LMNA
      • HGPS
      • EMD2
      • FPLD
      • CDCD1
      • LDP1
      • LGMD1B
      • IDC
      • CMT2B1
      • LMNC
      • FPL
      • PRO1
      • Lamin-A/C
      • LMN1
      • CMD1A
      • LFP
      • CDDC
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02545 # Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals.
      SIZE: 664 amino acids; 74139 Da
      SUBUNIT: Homodimer of lamin A and lamin C. Interacts with lamin- associated polypeptides IA, IB and TMPO-alpha, RB1 and with emerin. Proteolytically processed isoform A interacts with NARF.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. & The C-terminal 18 residues are removed by proteolytic cleavage in isoform A. Proteolytic cleavage requires prior farnesylation and absence of farnesylation blocks cleavage (By similarity).
      DISEASE: SwissProt: P02545 # Defects in LMNA are a cause of Emery-Dreifuss muscular dystrophy type 2 (EDMD2) [MIM:181350]. EDMD2 is an autosomal dominant disorder characterized by slowly progressive muscle wasting and weakness, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. & Defects in LMNA are a cause of Emery-Dreifuss muscular dystrophy type 3 (EDMD3) [MIM:604929]. EDMD3 is an autosomal recessive disorder characterized by early contractures, muscle wasting and weakness and cardiomyopathy. & Defects in LMNA are a cause of dilated cardiomyopathy 1A (CMD1A) [MIM:115200]. Dilated cardiomyopathy, a disorder characterized by cardiac dilation and reduced systolic function, represents an outcome of a heterogeneous group of inherited and acquired disorders. & Defects in LMNA are a cause of CMD1A with quadriceps myopathy [MIM:607920]. Inheritance is autosomal dominant and the phenotype severe. & Defects in LMNA are a cause of generalized lipoatrophy associated with diabetes, hepatic steatosis, hypertrophic cardiomyopathy and leukomelanodermic papules (LDHCP) [MIM:608056]. LDHCP is a disorder characterized by acquired generalized lipoatrophy with metabolic alterations, massive liver steatosis, distinctive cutaneous manifestations, and cardiac abnormalities involving both endocardium and myocardium. & Defects in LMNA are a cause of familial partial lipodystrophy (FPLD) [MIM:151660]; also known as familial partial lipodystrophy Dunnigan type. FPLD is an autosomal dominant disorder characterized by marked loss of subcutaneous adipose tissue from the extremities and trunk but by excess fat deposition in the head and neck. Frequently associated with profound insulin resistance, dyslipidemia, and diabetes. & Defects in LMNA are the cause of limb-girdle muscular dystrophy type 1B (LGMD1B) [MIM:159001]. LGMD1B is an autosomal dominantly inherited slowly progressive limb girdle muscular dystrophy, with age-related atrioventricular cardiac conduction disturbances and the absence of early contractures. & Defects in LMNA are the cause of Charcot-Marie-Tooth disease type 2B1 (CMT2B1) [MIM:605588]. CMT2B1 is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy or CMT1, and primary peripheral axonal neuropathy or CMT2. Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. CMT2B1 inheritance is autosomal recessive. & Defects in LMNA are the cause of Hutchinson-Gilford progeria syndrome (HGPS) [MIM:176670]. HGPS is a rare genetic disorder characterized by features reminiscent of marked premature aging. & Defects in LMNA are a cause of familial atrial fibrillation (ATFB) [MIM:607554]. Atrial fibrillation is a cardiac arrhythmia characterized by rapid and irregular activation of the atrium. It causes thromboembolism, tachycardia-mediated cardiomyopathy and heart failure. & Defects in LMNA are a cause of Werner syndrome (WRN) [MIM:277700]. WRN is an autosomal, recessively inherited, segmental progeroid syndrome, in which multiple aspects (or segments) of aging phenotypes seem to be entailed. The features of Werner syndrome are scleroderma-like skin changes, especially in the extremities, cataract, subcutaneous calcification, premature arteriosclerosis, diabetes mellitus, and a wizened and prematurely aged facies. & Defects in LMNA are the cause of mandibuloacral dysplasia with type A lipodystrophy (MADA) [MIM:248370]. Mandibuloacral dysplasia (MAD) is a rare autosomal recessive disorder characterized by mandibular and clavicular hypoplasia, acroosteolysis, delayed closure of the cranial suture, joint contractures, and types A or B patterns of lipodystrophy. Type A lipodystrophy observed in MADA, is characterized by fat loss restricted to the extremities. & Defects in LMNA are a cause of lethal tight skin contracture syndrome [MIM:275210]; also called restrictive dermopathy (RD). Lethal tight skin contracture syndrome is a rare disorder mainly characterized by intrauterine growth retardation, tight and rigid skin with erosions, prominent superficial vasculature and epidermal hyperkeratosis, facial features (small mouth, small pinched nose and micrognathia), sparse/absent eyelashes and eyebrows, mineralization defects of the skull, thin dysplastic clavicles, pulmonary hypoplasia, multiple joint contractures and an early neonatal lethal course. Liveborn children usually die within the first week of life. The overall prevalence of consanguineous cases suggested an autosomal recessive inheritance.
      SIMILARITY: SwissProt: P02545 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are three types of lamins in human cells: A, B, and C. & The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively.
      Molecular Weight~74/65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates from A431 cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this antibody detected Lamin A/C in 20 μg of RIPA lysates from A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Lamin A/C, clone 14 2465190
      Anti-Lamin A/C, clone 14 - 26011 26011
      Anti-Lamin A/C, clone 14 - 26488 26488
      Anti-Lamin A/C, clone 14 - 27781 27781
      Anti-Lamin A/C, clone 14 - 2939627 2939627
      Anti-Lamin A/C, clone 14 - 30202 30202
      Anti-Lamin A/C, clone 14 -2782080 2782080
      Anti-Lamin A/C, clone 14 Monoclonal Antibody 2880704

      References

      Reference overviewPub Med ID
      The bromodomain protein Brd4 insulates chromatin from DNA damage signalling.
      Floyd, SR; Pacold, ME; Huang, Q; Clarke, SM; Lam, FC; Cannell, IG; Bryson, BD; Rameseder, J; Lee, MJ; Blake, EJ; Fydrych, A; Ho, R; Greenberger, BA; Chen, GC; Maffa, A; Del Rosario, AM; Root, DE; Carpenter, AE; Hahn, WC; Sabatini, DM; Chen, CC; White, FM; Bradner, JE; Yaffe, MB
      Nature  498  246-50  2013

      Show Abstract
      23728299 23728299
      Ubiquitin-dependent recruitment of the Bloom syndrome helicase upon replication stress is required to suppress homologous recombination.
      Tikoo, S; Madhavan, V; Hussain, M; Miller, ES; Arora, P; Zlatanou, A; Modi, P; Townsend, K; Stewart, GS; Sengupta, S
      The EMBO journal  32  1778-92  2013

      Show Abstract
      23708797 23708797
      The c-Myc-regulated microRNA-17~92 (miR-17~92) and miR-106a~363 clusters target hCYP19A1 and hGCM1 to inhibit human trophoblast differentiation.
      Kumar, P; Luo, Y; Tudela, C; Alexander, JM; Mendelson, CR
      Molecular and cellular biology  33  1782-96  2013

      Show Abstract
      23438603 23438603
      Estrogen-related receptor gamma (ERRgamma) mediates oxygen-dependent induction of aromatase (CYP19) gene expression during human trophoblast differentiation.
      Kumar, P; Mendelson, CR
      Molecular endocrinology (Baltimore, Md.)  25  1513-26  2011

      Show Abstract
      21757507 21757507
      Both t-Darpp and DARPP-32 can cause resistance to trastuzumab in breast cancer cells and are frequently expressed in primary breast cancers.
      Sophie Hamel,Amélie Bouchard,Cristiano Ferrario,Saima Hassan,Adriana Aguilar-Mahecha,Marguerite Buchanan,Louise Quenneville,Wilson Miller,Mark Basik
      Breast cancer research and treatment  120  2010

      Show Abstract
      19301121 19301121
      DNA methyltransferase 1-associated protein (DMAP1) is a co-repressor that stimulates DNA methylation globally and locally at sites of double strand break repair.
      Lee, GE; Kim, JH; Taylor, M; Muller, MT
      The Journal of biological chemistry  285  37630-40  2010

      Show Abstract
      20864525 20864525
      A novel reverse transduction adenoviral array for the functional analysis of shRNA libraries.
      Oehmig, A; Klotzbücher, A; Thomas, M; Weise, F; Hagner, U; Brundiers, R; Waldherr, D; Lingnau, A; Knappik, A; Kubbutat, MH; Joos, TO; Volkmer, H
      BMC genomics  9  441  2008

      Show Abstract
      18816379 18816379
      p300 modulates nuclear morphology in prostate cancer
      Debes, Jose D, et al
      Cancer Res, 65:708-712 (2005)  2005

      15705864 15705864
      Distinct pools of epithelial sodium channels are expressed at the plasma membrane
      Hughey, Rebecca P, et al
      J Biol Chem, 279:48491-4 (2004)  2004

      15466477 15466477
      LMNA, encoding lamin A/C, is mutated in partial lipodystrophy.
      Shackleton, S, et al.
      Nat. Genet., 24: 153-6 (2000)  2000

      Show Abstract
      10655060 10655060

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies