05-435 | Anti-Lck Antibody, clone 3A5

05-435
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-435
      Replaces04-372
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Lck Antibody, clone 3A5
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: Jurkat cell lysate (12-303)
      PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationThis Anti-Lck Antibody, clone 3A5 is validated for use in IP, WB for the detection of Lck.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST-fusion to residues 1-225 of murine Lck
      Clone3A5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes Lck.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described.
      Gene Symbol
      • LCK
      • pp58lck
      • LSK
      • p56-LCK
      • p56lck
      • YT16
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06239 # Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR.


      SIZE: 509 amino acids; 58001 Da

      SUBUNIT: Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB.

      SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Present in lipid rafts in an unactive form.

      TISSUE SPECIFICITY: Expressed specifically in lymphoid cells.


      DOMAIN: SwissProt: P06239 The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.

      PTM: Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
      DISEASE:SwissProt: P06239 # A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.

      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight56 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from Jurkat cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Lck, clone 3A5 2454685
      Anti-Lck, clone 3A5 (mouse monoclonal IgG2b#954;) - 2120018 2120018
      Anti-Lck, clone 3A5 (mouse monoclonal IgG2bk) - 2092642 2092642
      Anti-Lck, clone 3A5 (mouse monoclonal IgG2bk) - 2199556 2199556
      Anti-Lck, clone 3A5 (mouse monoclonal IgG2bκ) 2976346
      Anti-Lck, clone 3A5 - 16188 16188
      Anti-Lck, clone 3A5 - 19367 19367
      Anti-Lck, clone 3A5 - 2042728 2042728
      Anti-Lck, clone 3A5 - 21179 21179
      Anti-Lck, clone 3A5 - 22708 22708

      References

      Reference overviewApplicationPub Med ID
      Association of SRC-related kinase Lyn with the interleukin-2 receptor and its role in maintaining constitutive phosphorylation of JAK/STAT in human T-cell leukemia virus type 1-transformed T cells.
      Shuh, M; Morse, BA; Heidecker, G; Derse, D
      Journal of virology  85  4623-7  2011

      Show Abstract
      Western Blotting21345943 21345943
      The differential expression of LCK and BAFF-receptor and their role in apoptosis in human lymphomas.
      Jennifer C Paterson, Sara Tedoldi, Andrew Craxton, Margaret Jones, Martin-Leo Hansmann, Graham Collins, Helen Roberton, Yasodha Natkunam, Stefano Pileri, Elias Campo, Edward A Clark, David Y Mason, Teresa Marafioti
      Haematologica  91  772-80  2006

      Show Abstract
      16769579 16769579
      The Lck tyrosine kinase is expressed in brain neurons.
      Omri, B, et al.
      J. Neurochem., 67: 1360-4 (1996)  1996

      Show Abstract
      8858916 8858916

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      Included Positive Control

      Catalogue Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies