04-539 | Anti-MAPKAP-K2 Antibody, clone 7H4.2

200 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBMPurifiedMonoclonal Antibody
      Catalogue Number04-539
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-MAPKAP-K2 Antibody, clone 7H4.2
      Alternate Names
      • MAPK-activated protein kinase 2
      • MAPKAP kinase 2
      Product Information
      Presentation200 µg (1 mg/mL) of mouse monoclonal IgG in PBS containing 0.1% sodium azide.
      ApplicationAnti-MAPKAP-K2 Antibody, clone 7H4.2 detects level of MAPKAP-K2 & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesSuggested dilutions:
      Immunoblot Analysis: 1:500
      Immunoprecipitation: 1:20
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide of the C-terminus of the long form of human MAPKAP-K2.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
      Gene Symbol
      • MAPKAPK2
      • MK2
      • MAPKAPK-2
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P49137 # Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at 'Ser-7' and tyrosine hydroxylase (on 'Ser-19' and 'Ser-40'). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity.
      SIZE: 400 amino acids; 45568 Da
      SUBUNIT: Interacts with PHC2.
      TISSUE SPECIFICITY: Expressed in all tissues examined.
      PTM: Phosphorylated and activated by MAP kinase.
      SIMILARITY: SwissProt: P49137 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain.
      Molecular Weight45 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti- MAPKAP Kinase 2, clone 7H4.2 (Mouse monoclonal IgG) 3037972
      Anti- MAPKAP Kinase 2, clone 7H4.2 (Mouse monoclonal IgG) - 2015401 2015401
      Anti- MAPKAP Kinase 2, clone 7H4.2 (Mouse monoclonal IgG) - 2239833 2239833
      Anti- MAPKAP Kinase 2, clone 7H4.2 - 2425926 2425926
      Anti- MAPKAP Kinase 2, clone 7H4.2 - 2507231 2507231
      Anti- MAPKAP Kinase 2, clone 7H4.2 - DAM1430012 DAM1430012
      Anti- MAPKAP Kinase 2, clone 7H4.2 - JBC1889916 JBC1889916
      Anti- MAPKAP Kinase 2, clone 7H4.2 - NG1896100 NG1896100
      Anti- MAPKAP Kinase 2, clone 7H4.2 -2608735 2608735
      Anti- MAPKAP Kinase 2, clone 7H4.2 -2851181 2851181


      Reference overviewPub Med ID
      MAPKAPK2 and HSP27 are downstream effectors of p38 MAP kinase-mediated matrix metalloproteinase type 2 activation and cell invasion in human prostate cancer.
      Xu, L, et al.
      Oncogene, 25: 2987-98 (2006)  2006

      Show Abstract
      16407830 16407830
      MAPK-activated protein kinase 2 deficiency in microglia inhibits pro-inflammatory mediator release and resultant neurotoxicity. Relevance to neuroinflammation in a transgenic mouse model of Alzheimer disease.
      Culbert, Ainsley A, et al.
      J. Biol. Chem., 281: 23658-67 (2006)  2006

      Show Abstract
      16774924 16774924
      CDC25B phosphorylation by p38 and MK-2
      Lemaire, Matthieu, et al
      Cell Cycle, 5:1649-53 (2006)  2006

      16861915 16861915
      MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation.
      Manke, Isaac A, et al.
      Mol. Cell, 17: 37-48 (2005)  2005

      Show Abstract
      15629715 15629715