05-337 | Anti-MARCKS Antibody, clone 2F12

05-337
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, HIP, WBMAscitesMonoclonal Antibody
      Description
      Catalogue Number05-337
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-MARCKS Antibody, clone 2F12
      References
      Product Information
      FormatAscites
      Presentationmouse ascites containing 0.05% sodium azide
      Applications
      ApplicationAnti-MARCKS Antibody, clone 2F12 detects level of MARCKS & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenPurified recombinant human MARCKS; Epitope mapped to the carboxyl-terminal.
      Clone2F12
      HostMouse
      SpecificityMARCKS
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis.
      Gene Symbol
      • MARCKS
      • 80K-L
      • MACS
      • PRKCSL
      • FLJ14368
      • phosphomyristin
      • MRACKS
      • PKCSL
      • FLJ90045
      Purification MethodAscites
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29966 # MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
      SIZE: 332 amino acids; 31555 Da
      PTM: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
      SIMILARITY: SwissProt: P29966 ## Belongs to the MARCKS family.
      Molecular Weightnone given
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on bovine brain extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -20°C
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2135164 2135164
      Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2366548 2366548
      Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2382138 2382138
      Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2390491 2390491
      Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2392258 2392258
      Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2428644 2428644
      Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2066049 2066049
      Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2196187 2196187
      Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2198983 2198983
      Anti-MARCKS, clone 2F12 (mouse monoclonal IgG1) - 2262767 2262767

      References

      Reference overviewApplicationPub Med ID
      20-Hydroxyeicosatetraenoic acid-induced vasoconstriction and inhibition of potassium current in cerebral vascular smooth muscle is dependent on activation of protein kinase C
      Lange, A., et al
      J Biol Chem, 272:27345-52 (1997)  1997

      Immunoblotting (Western)9341185 9341185

      FAQ

      QuestionAnswer
      What is the concentration of this antibody?We are not able to quantitate ascites.
      What is the molecular weight of MARCKS?MARCKS is a protein that can rang in apparent molecular weight from 32-92kDa depending on the cell or tissue extract. It's unique modified amino acid primary structure and multiple PKC phosphorylation sites account for the anomalous mobility during SDS-PAGE, which can explain the slight variance in apparent molecular weight.

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies