07-199 Anti-MBD2/3 Antibody

07-199
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-199
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-MBD2/3 Antibody
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect MBD2/3 with Anti-MBD2/3 Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 152-262 of mouse MBD2a
      HostRabbit
      SpecificityMBD2a, MBD2b, MBD3; does not cross-react with MBD1 or MBD4
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryDNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
      Gene Symbol
      • MBD2
      • DKFZp586O0821
      • Demethylase
      • NY-CO-41
      • DMTase
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UBB5 # Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemi-methylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Isoform 1 may enhance the activation of some unmethylated cAMP-responsive promoters. Reports about DNA demethylase activity of isoform 2 are contradictory.
      SIZE: 411 amino acids; 43255 Da
      SUBUNIT: Heterodimer with MBD3. Part of the MeCP1 complex that contains HDAC1 and HDAC2. Binds DNMT1, MIZF, XAB1, SIN3A, p66- alpha and p66-beta. Isoform 1 binds DHX9, but isoform 2 does not.
      SUBCELLULAR LOCATION: Nucleus. Note=Nuclear, in discrete foci. Detected at replication foci in late S phase.
      TISSUE SPECIFICITY: Highly expressed in brain, heart, kidney, stomach, testis and placenta.
      SIMILARITY: SwissProt: Q9UBB5 ## Contains 1 MBD (methyl-CpG-binding) domain.
      Molecular Weight50 kDa (MBD2a), 33 kDa (MBD2b) and 35 kDa (MBD3)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on Hela nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-MBD2/3 - 2038920389
      Anti-MBD2/3 - 2983229832

      References

      Reference overviewApplicationPub Med ID
      The methylated-DNA binding protein MBD2 enhances NGFI-A (egr-1)-mediated transcriptional activation of the glucocorticoid receptor.
      Weaver, IC; Hellstrom, IC; Brown, SE; Andrews, SD; Dymov, S; Diorio, J; Zhang, TY; Szyf, M; Meaney, MJ
      Philosophical transactions of the Royal Society of London. Series B, Biological sciences  369  2014

      Show Abstract
      25135974 25135974
      MeCP2 knockdown reveals DNA methylation-independent gene repression of target genes in living cells and a bias in the cellular location of target gene products.
      Shinya Yakabe,Hidenobu Soejima,Hitomi Yatsuki,Hirotaka Tominaga,Wei Zhao,Ken Higashimoto,Keiichiro Joh,Shinichi Kudo,Kohji Miyazaki,Tsunehiro Mukai
      Genes & genetic systems  83  2008

      Show Abstract
      18506103 18506103
      Histone modifications silence the GATA transcription factor genes in ovarian cancer.
      Caslini, C; Capo-chichi, CD; Roland, IH; Nicolas, E; Yeung, AT; Xu, XX
      Oncogene  25  5446-61  2006

      Show Abstract
      16607277 16607277
      Methyl-CpG-binding domain protein-2 mediates transcriptional repression associated with hypermethylated GSTP1 CpG islands in MCF-7 breast cancer cells
      Lin, X. and Nelson, W. G.
      Cancer Res, 63:498-504 (2003)  2003

      Immunoblotting (Western)12543808 12543808
      Active repression of methylated genes by the chromosomal protein MBD1.
      Ng, H H, et al.
      Mol. Cell. Biol., 20: 1394-406 (2000)  2000

      Show Abstract
      10648624 10648624

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