05-1572 | Anti-MDC1 Antibody, clone P2B11

05-1572
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Chp, BICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1572
      DescriptionAnti-MDC1 Antibody, clone P2B11
      Alternate Names
      • Nuclear factor with BRCT domains 1
      • homologue to Drosophila photoreceptor protein calphotin
      • mediator of DNA damage checkpoint 1
      Background InformationMDC1 is a subunit of the cohesin complex and is an essential protein required for sister chromatid cohesion in mitosis and meiosis. Temperature-sensitive mutations of this protein have been associated with defects in sister chromatid cohesion, chromosome condensation, and chromosome segregation. Its expression is cell cycle regulated and peaks in S phase. Expression of MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 has been shown to interact with H2A.X near sites of DNA double-strand breaks and recruit ATM kinase to the damaged region of DNA. Consistent with this role, MDC1 also appears to modulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2.
      References
      Product Information
      FormatPurified
      Control
      • NIH/3T3 cells
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) without 0.05% sodium azide.
      Applications
      ApplicationAnti-MDC1 Antibody, clone P2B11 is a Mouse Monoclonal Antibody for detection of MDC1 also known as Nuclear factor with BRCT domains 1 & has been validated in ICC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a previous lot detected MDC1 in NIH/3T3 cells.

      Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Lou, Z., et al. (2003). Nature. 421(6926):957-961.)
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.
      EpitopeUnknown
      CloneP2B11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes MDC1 at and around the N-terminus.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      • Chimpanzee
      • Bovine
      Species Reactivity NoteDemonstrated to react with mouse.
      Predicted to react with human, bovine (cow) and chimpanzee based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 7 divergent copies of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. Mice with mutations in this gene exhibit growth retardation, male infertility, immune defects, chromosome instability, DNA repair defects, and radiation sensitivity. [provided by RefSeq]
      Gene Symbol
      • MDC1
      • NFBD1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1/CHK1 and CHEK2/CHK2/CDS1 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 By similarity.

      SUBUNIT STRUCTURE: Interacts with several proteins involved in the DNA damage response, although not all these interactions may be direct. Interacts with CHEK2/CHK2/CDS1, which requires ATM-mediated phosphorylation within the FHA domain of CHEK2. Interacts constitutively with the BRCA1-BARD1 complex, SMC1A and TP53BP1. Interacts with ATM and FANCD2, and these interactions are reduced upon DNA damage. Also interacts with the PRKDC complex, composed of G22P1/KU70, XRCC5/KU80 and PRKDC/XRCC7. This interaction may be required for PRKDC autophosphorylation, which is essential for DNA double strand break (DSB) repair. When phosphorylated by ATM, interacts with RNF8. Interacts with CEP164 By similarity. Interacts with H2AFX, which requires phosphorylation of H2AFX. Interacts with the MRN complex, composed of MRE11A/MRE11, RAD50, and NBN.

      SUBCELLULAR LOCATION: Nucleus. Note: Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires phosphorylation of H2AFX. Ref.1

      DOMAIN: Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AFX By similarity.

      PTM: Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint By similarity.

      SEQUENCE SIMILARITIES: Contains 2 BRCT domains.

      Contains 1 FHA domain.
      Molecular Weight ~ 184 kDa. There are 4 isoforms of human MDC1 because of splicing with molecular weights of approximately 227, 198, 196, and 117 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-MDC1, clone P2B11 - 2446831 2446831
      Anti-MDC1, clone P2B11 - 1996266 1996266
      Anti-MDC1, clone P2B11 - 2066322 2066322
      Anti-MDC1, clone P2B11 - 2262742 2262742
      Anti-MDC1, clone P2B11 - 2330616 2330616
      Anti-MDC1, clone P2B11 - NG1734259 NG1734259
      Anti-MDC1, clone P2B11 - NG1842843 NG1842843
      Anti-MDC1, clone P2B11 - NG1901122 NG1901122
      Anti-MDC1, clone P2B11 - NRG1675254 NRG1675254
      Anti-MDC1, clone P2B11 -2500504 2500504

      References

      Reference overviewPub Med ID
      ATR and a Chk1-Aurora B pathway coordinate postmitotic genome surveillance with cytokinetic abscission.
      Mackay, DR; Ullman, KS
      Molecular biology of the cell  26  2217-26  2015

      Show Abstract
      25904336 25904336