05-765 | Anti-MLL/HRX Antibody, CT., clone 9-12

05-765
200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MChIP, IF, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-765
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-MLL/HRX Antibody, CT., clone 9-12
      Alternate Names
      • Histone-lysine N-methyltransferase 2A
      • Lysine N-methyltransferase 2A
      • ALL-1
      • CXXC-type zinc finger protein 7
      • Myeloid/lymphoid or mixed-lineage leukemia
      • Myeloid/lymphoid or mixed-lineage leukemia protein 1
      • Trithorax-like protein
      • Zinc finger protein HRX
      • MLL cleavage product C180
      • C-terminal cleavage product of 180 kDa
      • p180
      Background InformationThe acute lymphoblastic leukaemia (ALL)-1 (also known as MLL, HRX, HTRX, and TRX1) gene on human chromosome 11q23 is the site of many locally clustered chromosomal alterations associated with several types of acute leukaemias, including deletions, partial duplications and translocations. Structurally variant proteins derived from the altered gene presumably cause the malignant transformation of early haemopoietic progenitor cells.
      References
      Product Information
      FormatPurified
      Control
      • K562 nuclear extract
      PresentationPurified mouse IgG1 in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC
      Applications
      ApplicationAnti-MLL/HRX, C-term., clone 9-12 is a high quality mouse monoclonal antibody that has been validated and published for use in Chromatin Immunoprecipitation (ChIP), Immunofluorescence, Immunoprecipitation, and Western Blotting applications.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmnuofluorescence Analysis: A representative lot detected MLL expression in SOX2+ cells in paraffin-embedded high-grade human gliomas sections (Gallo, M., et al. (2013). Cancer Res. 73(1):417-427).

      Chromatin Immnuoprecipitation (ChIP) Analysis: A representative lot detected MLL occupancy at the Hoxa10 and Meis promoters in mouse MLL-AF10 leukemia cells (Gallo, M., et al. (2013). Cancer Res. 73(1):417-427).

      Chromatin Immnuoprecipitation (ChIP) Analysis: A representative lot detected an enhanced MLL occupancy at the Ink4a locus of 8-month-old than 2-month-old bEzTG mouse islets. The same age-dependent Ink4a locus enrichment was observed with H3K4me3, while the opposite trend was seen with Ezh and H3K27me3 enrichment at the same locus (Zhou, J.X., et al. (2013). J. Clin. Invest. 123(11):4849-4858).

      Chromatin Immnuoprecipitation (ChIP) Analysis: A representative lot detected MLL occupancy at the HOXA10 promoter region in G179NS and G411NS human glioblastoma neural stem cells (Gallo, M., et al. (2013). Cancer Res. 73(1):417-427).

      Chromatin Immnuoprecipitation (ChIP) Analysis: A representative lot detected MLL occupancy at the DNA replication origin (RD) as well as at both exon 1b and p16INK4a/p19ARF shared exon 2 in mouse embryonic fibroblast (MEF). An increased MLL enrichment at these sites was observed in senescent and Polycomb mutant MEFs (Agherbi, H., et al. (2009). PLoS One. 4(5):e5622).

      Chromatin Immnuoprecipitation (ChIP) Analysis: A representative lot detected MLL occupancy at the Hoxa9 AB region using mouse embryonic fibroblast (MEF) chromatin preparation (Erfurth, F.E., et al. (2008). Proc. Natl. Acad. Sci. U.S.A. 105(21):7517-7522).

      Immnuoprecipitation Analysis: A representative lot co-immunoprecipitated JmjD3 and RbBP5, but not Dnmt3a, with MLL from Min6 mouse insulinoma cell extract (Zhou, J.X., et al. (2013). J. Clin. Invest. 123(11):4849-4858).

      Western Blotting Analysis: A representative lot detected higher MLL level in cultured glioblastoma neural stem (GNS) cells than neural stem (NS) cells, as well as MLL enrichment in the CD15+ fraction of freshly resected Glioblastoma (GBM) cells (Gallo, M., et al. (2013). Cancer Res. 73(1):417-427).

      Western Blotting Analysis: A representative lot detected SET domain-containing C-terminal fragment of the MLL complex enzymatic subunit MLL (C180; MLLC) in anti-FLAG immunoprecipitate from HeLaS cells stably expressing FLAG-tagged hDPY-30 (Cho, Y.W., et al. (2007). J. Biol. Chem. 282(28):20395-20406).
      Biological Information
      ImmunogenMBP-tagged recombinant human mixed lineage leukemia (MLL) protein C-terminal fragment (a.a. 3084-3959).
      EpitopeMLL C-terminal fragment
      Clone9-12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis monoclonal antibody targets the C-terminal proteolytic fragment of MLL (C180; MLLC). A reduced target band detection by this antibody was seen lysate from G411NS human glioblastoma neural stem cells transfected with MLL shRNA (Gallo, M., et al. (2013). Cancer Res. 73(1):417-427).
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KMT2A
      • ALL1
      • CXXC7
      • HRX
      • HTRX
      • MLL
      Purification MethodProtein G purified.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q03164 # Possibly acts as a transcriptional regulatory factor.
      SIZE: 3969 amino acids; 431764 Da
      SUBUNIT: Interacts with SBF1.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Heart, lung, brain and T- and B-lymphocytes.
      DISEASE: SwissProt: Q03164 # Chromosomal aberrations involving MLL are a cause of acute leukemias. Translocation t(1;11)(q21;q23) with MLLT11/AF1Q; translocation t(3;11)(p21;q23) with NCKIPSD/AF3p21; translocation t(3,11)(q25,q23) with GMPS; translocation t(4;11)(q21;q23) with AFF1/MLLT2/AF4; insertion ins(5;11)(q31;q13q23) with AFF4/AF5Q31; translocation t(5;11)(q12;q23) with AF5-alpha/CENPK; translocation t(6;11)(q27;q23) with MLLT4/AF6; translocation t(9;11)(p22;q23) with MLLT3/AF9; translocation t(10;11)(p11.2;q23) with ABI1; translocation t(10;11)(p12;q23) with MLLT10/AF10; t(11;15)(q23;q14) with CASC5 and ZFYVE19; translocation t(11;17)(q23;q21) with MLLT6/AF17; translocation t(11;19)(q23;p13.3) with ELL; translocation t(11;19)(q23;p13.3) with MLLT1/ENL; translocation t(11;19)(q23;p23) with GAS7; translocation t(X;11)(q13;q23) with FOXO4/AFX1. Translocation t(3;11)(q28;q23) with LPP. Translocation t(10;11)(q22;q23) with CXXC6. Translocation t(9;11)(q34;q23) with DAB2IP. Translocation t(4;11)(p12;q23) with FRYL. & A chromosomal aberration involving MLL may be a cause of chronic neutrophilic leukemia. Translocation t(4;11)(q21;q23) with SEPT11.
      SIMILARITY: SwissProt: Q03164 ## Belongs to the TRX/MLL family. & Contains 3 A.T hook DNA-binding domains. & Contains 1 bromo domain. & Contains 1 CXXC-type zinc finger. & Contains 3 PHD-type zinc fingers. & Contains 1 post-SET domain. & Contains 1 SET domain.
      Molecular Weight~180 kDa observed. 134.4 kDa (a.a. 2719-3969; human MLL cleavage product C180) and 431.8/427.7/432.1 kDa (human full-length MLL isoform 1/2 (14P-18B)/3) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 nuclear extract.
      Western Blotting Analysis: 0.1-1 µg/mL of this antibody detected MLL C-terminal fragment (C180; MLLC) in K562 nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore for 2 years at -20ºC from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-MLL/HRX, C-term., clone 9-12 - 0608038481 0608038481
      Anti-MLL/HRX, C-term., clone 9-12 - 26788 26788
      Anti-MLL/HRX, clone 9-12 -2724347 2724347
      Anti-MLL/HRX, clone 9-12 -2768451 2768451
      Anti-MLL/HRX, clone 9-12 Monoclonal Antibody 2923199
      Anti-MLLC/HRX, clone 9-12 2477014
      Anti-MLLC/HRX, clone 9-12 (mouse monoclonal IgG1) - 2391019 2391019
      Anti-MLLC/HRX, clone 9-12 (mouse monoclonal IgG1) - 1957277 1957277
      Anti-MLLC/HRX, clone 9-12 (mouse monoclonal IgG1) - 2018113 2018113
      Anti-MLLC/HRX, clone 9-12 - 2155408 2155408

      References

      Reference overviewApplicationPub Med ID
      A tumorigenic MLL-homeobox network in human glioblastoma stem cells.
      Gallo, M; Ho, J; Coutinho, FJ; Vanner, R; Lee, L; Head, R; Ling, EK; Clarke, ID; Dirks, PB
      Cancer research  73  417-27  2013

      Show Abstract
      Western Blotting23108137 23108137
      Epigenetic Roles of MLL Oncoproteins Are Dependent on NF-κB.
      Kuo, Hsu-Ping, et al.
      Cancer Cell, (2013)  2013

      Show Abstract
      Chromatin Immunoprecipitation (ChIP)24054986 24054986
      Combined modulation of polycomb and trithorax genes rejuvenates β cell replication.
      Zhou, JX; Dhawan, S; Fu, H; Snyder, E; Bottino, R; Kundu, S; Kim, SK; Bhushan, A
      The Journal of clinical investigation  123  4849-58  2013

      Show Abstract
      24216481 24216481
      Chromatin loading of E2F-MLL complex by cancer-associated coregulator ANCCA via reading a specific histone mark.
      Revenko, AS; Kalashnikova, EV; Gemo, AT; Zou, JX; Chen, HW
      Molecular and cellular biology  30  5260-72  2010

      Show Abstract Full Text Article
      20855524 20855524
      Polycomb mediated epigenetic silencing and replication timing at the INK4a/ARF locus during senescence.
      Agherbi, H; Gaussmann-Wenger, A; Verthuy, C; Chasson, L; Serrano, M; Djabali, M
      PloS one  4  e5622  2009

      Show Abstract Full Text Article
      Immunoprecipitation19462008 19462008
      MLL protects CpG clusters from methylation within the Hoxa9 gene, maintaining transcript expression.
      Erfurth, FE; Popovic, R; Grembecka, J; Cierpicki, T; Theisler, C; Xia, ZB; Stuart, T; Diaz, MO; Bushweller, JH; Zeleznik-Le, NJ
      Proceedings of the National Academy of Sciences of the United States of America  105  7517-22  2008

      Show Abstract
      18483194 18483194
      PTIP associates with MLL3- and MLL4-containing histone H3 lysine 4 methyltransferase complex.
      Cho, YW; Hong, T; Hong, S; Guo, H; Yu, H; Kim, D; Guszczynski, T; Dressler, GR; Copeland, TD; Kalkum, M; Ge, K
      The Journal of biological chemistry  282  20395-406  2007

      Show Abstract Full Text Article
      17500065 17500065
      Leukemia proto-oncoprotein MLL is proteolytically processed into 2 fragments with opposite transcriptional properties.
      Yokoyama, Akihiko, et al.
      Blood, 100: 3710-8 (2002)  2002

      Show Abstract
      12393701 12393701

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      05-764 Anti-MLL/HRX Antibody, NT., clone N4.4 Show Pricing & Availability
      ABE240 Anti-MLL-C Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies