06-1036 | Anti-March5 Antibody

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbSerumPolyclonal Antibody
      Catalogue Number06-1036
      DescriptionAnti-March5 Antibody
      Alternate Names
      • membrane-associated ring finger (C3HC4) 5
      • Membrane-associated RING finger protein 5
      • RING finger protein 153
      • E3 ubiquitin-protein ligase MARCH5
      • Mitochondrial ubiquitin ligase
      • Membrane-associated RING-CH protein V
      Background InformationMembrane-associated RING-CH protein 5 (March5), also known as NKP66, is thought to be a mitochondrial ubiquitin ligase that is localized on the mitochondrial membrane. March5 has a Plant Homeo-Domain (PHD) motif that is essential for E3 ubiquitin ligase action. MARCH 5 is also thought to have four transmembrane domains. March5 has been associated with mitochondrial fission and division. March5 also seems to have a defensive role against mitochondrial dysfunction by interacting with mutant superoxide dismutase 1 (mSOD1) and preventing mSOD1 from causing damage. mSOD1 damage has been linked to the onset and progression of amyotrophic lateral sclerosis.
      Product Information
      • HL-60 cell lysate
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      ApplicationAnti-March5 Antibody is a Rabbit Polyclonal Antibody for detection of March5 also known as Membrane-associated RING finger protein 5 & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot of this antibody was used by an independent laboratory in IC. (Korfali, N., et al. (2010). Mol Cell Proteomics. 9:2571-2585.)
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human March5.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySummary: March5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).[supplied by OMIM].
      Gene Symbol
      • MITOL
      • MARCH-V
      • RNF153
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.

      PATHWAY: Protein modification; protein ubiquitination.

      SUBUNIT STRUCTURE: Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1.

      SUBCELLULAR LOCATION: Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane. Note: Authors show that the protein can be detected in endoplasmic reticulum. Authors show its presence only in mitochondria.

      TISSUE SPECIFICITY: Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle.

      DOMAIN: The RING-CH-type zinc finger domain is required for E3 ligase activity (by similarity).

      POST-TRANSLATIONAL MODIFICATION: Autoubiquitinated leading to degradation (short half-life).

      MISCELLANEOUS: By binding to and ubiquitinating two ALS1 variants of SOD1 (mSOD1 variants Arg-86 and Ala-94) it attenuates their cytotoxicity.

      SEQUENCE SIMILARITIES: Contains 1 RING-CH-type zinc finger.
      Molecular Weight ~52 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HL-60 cell lysate.

      Western Blot Analysis: A 1:2,000 dilution of this antibody detected March5 on 10 µg of HL-60 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-March5 - 2227182 2227182
      Anti-March5 - NRG1857445 NRG1857445
      Anti-March5 -2719446 2719446
      Anti-March5 -2767031 2767031
      Anti-March5 Polyclonal Antibody 3042013
      Anti-March5 Polyclonal Antibody 2925726


      Reference overviewApplicationPub Med ID
      The leukocyte nuclear envelope proteome varies with cell activation and contains novel transmembrane proteins that affect genome architecture.
      Korfali, N; Wilkie, GS; Swanson, SK; Srsen, V; Batrakou, DG; Fairley, EA; Malik, P; Zuleger, N; Goncharevich, A; de Las Heras, J; Kelly, DA; Kerr, AR; Florens, L; Schirmer, EC
      Molecular & cellular proteomics : MCP  9  2571-85  2010

      Show Abstract
      Western Blotting20693407 20693407