05-175 | Anti-Myb Antibody, clone 1-1

05-175
250 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-175
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Myb Antibody, clone 1-1
      Alternate Names
      • Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA)
      • v-myb avian myeloblastosis viral oncogene homolog
      • v-myb avian myeloblastosis viral oncogene homolog
      • v-myb myeloblastosis viral oncogene homolog
      • v-myb myeloblastosis viral oncogene homolog (avian)
      Background InformationMyb is a transcriptional activator. It is a DNA-binding protein that specifically recognizes the sequence 5'-YAAC[GT]G-3'. Myb plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate.
        Included Positive Antigen Control: Catalog # 12-303, Jurkat cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationAnti-Myb Antibody, clone 1-1 is a Mouse Monoclonal Antibody for detection of Myb also known as Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA) & has been validated in IHC, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μg of previous lot immunoprecipitated Myb from Jurkat cell lysate.

      Immunohistochemistry:
      Biological Information
      ImmunogenRecombinant murine Myb purified by SDS-PAGE (Nicot, C., 2001) Clone 1-1.
      Clone1-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Myb, Mr 80 kDa, containing C-terminal amino acids from 401-636 (Salomoni, P., 1997).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYB
      • C-myb
      • c-myb13A_CDS
      • c-myb8B_CDS
      • c-myb_CDS
      • v-myb
      • c-myb14A_CDS
      • Cmyb
      • c-myb
      • efg
      • c-myb10A_CDS
      Purification MethodAmmonium sulfate precipitation, gel filtration, and ion-exchange chromatography.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P10242 # Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.
      SIZE: 640 amino acids; 72341 Da
      SUBUNIT: Binds MYBBP1A. Interacts with HIPK2 and NLK (By similarity).
      SUBCELLULAR LOCATION: Nucleus.
      DOMAIN: SwissProt: P10242 Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C- terminal domain involved in transcriptional repression.
      PTM: Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation (Probable). & Phosphorylated by NLK on multiple sites, which induces proteasomal degradation (By similarity).
      SIMILARITY: Contains 3 HTH myb-type DNA-binding domains.
      Molecular Weight80 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated on RIPA lysate from Jurkat cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected Myb in 20 μg of RIPA lysate from Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Myb, clone 1-1 2462781
      Anti-Myb, clone 1-1 (mouse monoclonal IgG2ak) - DAM1394748 DAM1394748
      Anti-Myb, clone 1-1 - 2426471 2426471
      Anti-Myb, clone 1-1 - 0609040588 0609040588
      Anti-Myb, clone 1-1 - 15588 15588
      Anti-Myb, clone 1-1 - 17319 17319
      Anti-Myb, clone 1-1 - 18305 18305
      Anti-Myb, clone 1-1 - 19601 19601
      Anti-Myb, clone 1-1 - 1983670 1983670
      Anti-Myb, clone 1-1 - 2034684 2034684

      References

      Reference overviewApplicationPub Med ID
      Cell cycle dependent oscillatory expression of estrogen receptor-α links Pol II elongation to neoplastic transformation.
      Vantaggiato, C; Tocchetti, M; Cappelletti, V; Gurtner, A; Villa, A; Daidone, MG; Piaggio, G; Maggi, A; Ciana, P
      Proceedings of the National Academy of Sciences of the United States of America  111  9561-6  2014

      Show Abstract
      24979764 24979764
      Direct repression of MYB by ZEB1 suppresses proliferation and epithelial gene expression during epithelial-to-mesenchymal transition of breast cancer cells.
      Hugo, HJ; Pereira, L; Suryadinata, R; Drabsch, Y; Gonda, TJ; Gunasinghe, NP; Pinto, C; Soo, ET; van Denderen, BJ; Hill, P; Ramsay, RG; Sarcevic, B; Newgreen, DF; Thompson, EW
      Breast cancer research : BCR  15  R113  2013

      Show Abstract
      24283570 24283570
      The inhibitory role of Mir-29 in growth of breast cancer cells.
      Wu, Z; Huang, X; Huang, X; Zou, Q; Guo, Y
      Journal of experimental & clinical cancer research : CR  32  98  2013

      Show Abstract
      Western Blotting24289849 24289849
      The TAL1 complex targets the FBXW7 tumor suppressor by activating miR-223 in human T cell acute lymphoblastic leukemia.
      Mansour, MR; Sanda, T; Lawton, LN; Li, X; Kreslavsky, T; Novina, CD; Brand, M; Gutierrez, A; Kelliher, MA; Jamieson, CH; von Boehmer, H; Young, RA; Look, AT
      The Journal of experimental medicine  210  1545-57  2013

      Show Abstract
      23857984 23857984
      c-Myb inhibits myoblast fusion.
      Kaspar, P; Ilencikova, K; Zikova, M; Horvath, O; Cermak, V; Bartunek, P; Strnad, H
      PloS one  8  e76742  2013

      Show Abstract
      24204667 24204667
      Perturbation of fetal hematopoiesis in a mouse model of Down syndrome's transient myeloproliferative disorder.
      Birger, Y; Goldberg, L; Chlon, TM; Goldenson, B; Muler, I; Schiby, G; Jacob-Hirsch, J; Rechavi, G; Crispino, JD; Izraeli, S
      Blood  122  988-98  2013

      Show Abstract
      23719302 23719302
      Activation of miR200 by c-Myb depends on ZEB1 expression and miR200 promoter methylation.
      Pieraccioli, M; Imbastari, F; Antonov, A; Melino, G; Raschellà, G
      Cell cycle (Georgetown, Tex.)  12  2309-20  2013

      Show Abstract
      Western Blotting24067373 24067373
      Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development.
      Timblin, GA; Schlissel, MS
      Journal of immunology (Baltimore, Md. : 1950)  191  4676-87  2013

      Show Abstract
      24068669 24068669
      Glioma pathogenesis-related protein 1 induces prostate cancer cell death through Hsc70-mediated suppression of AURKA and TPX2.
      Li, L; Yang, G; Ren, C; Tanimoto, R; Hirayama, T; Wang, J; Hawke, D; Kim, SM; Lee, JS; Goltsov, AA; Park, S; Ittmann, MM; Troncoso, P; Thompson, TC
      Molecular oncology  7  484-96  2013

      Show Abstract
      Western Blotting23333597 23333597
      c-Myb interacts with the glucocorticoid receptor and regulates its level in pre-B-acute lymphoblastic leukemia cells.
      Sarvaiya, PJ; Schwartz, JR; Geng, CD; Vedeckis, WV
      Molecular and cellular endocrinology  361  124-32  2012

      Show Abstract
      22516378 22516378

      Related Products & Applications

      Included Positive Control

      Catalogue Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies