06-549 | Anti-Myc Tag Antibody

06-549
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-549
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Myc Tag Antibody
      Alternate Names
      • Transcription factor p64
      • avian myelocytomatosis viral oncogene homolog
      • myc proto-oncogene protein
      • v-myc avian myelocytomatosis viral oncogene homolog
      • v-myc myelocytomatosis viral oncogene homolog (avian)
      Background InformationEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
      References
      Product Information
      FormatPurified
      Control
      • Positive control for human Myc: widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
      PresentationPurified rabbit in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationAnti-Myc Tag Antibody is an antibody against Myc Tag for use in IC & WB.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry:
      This antibody is reported to detect Myc-tagged proteins in transfected cells.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues 409-420 of human Myc (MEQKLISEEDLN).
      Epitopea.a. 409-420
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes and is specific for human Myc and recombinant proteins containing the Myc epitope tag.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Other species cross-reactivity not tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
      Gene Symbol
      • MYC
      • C-MYC
      • c-Myc
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
      SIZE: 439 amino acids; 48804 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated by PRKDC.
      DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular WeightPredicted molecular weight for human Myc: 49 kDa. Obeserved molecular weight may vary.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

      Western Blotting:
      1:500 dilution of this antibody detected myc-PP2A on 10 μg of Myc-PP2A A subunit transfected NIH/3T3 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Myc Tag 2475732
      Anti-Myc Tag - 2387827 2387827
      Anti-Myc Tag - 14966 14966
      Anti-Myc Tag - 17581 17581
      Anti-Myc Tag - 1977379 1977379
      Anti-Myc Tag - 20544 20544
      Anti-Myc Tag - 21727 21727
      Anti-Myc Tag - 22335 22335
      Anti-Myc Tag - 2277852 2277852
      Anti-Myc Tag - 23518 23518

      References

      Reference overviewApplicationPub Med ID
      Robo2 acts in trans to inhibit Slit-Robo1 repulsion in pre-crossing commissural axons.
      Evans, TA; Santiago, C; Arbeille, E; Bashaw, GJ
      eLife  4  e08407  2015

      Show Abstract
      26186094 26186094
      PAT1 inversely regulates the surface Amyloid Precursor Protein level in mouse primary neurons.
      Dilsizoglu Senol, A; Tagliafierro, L; Huguet, L; Gorisse-Hussonnois, L; Chasseigneaux, S; Allinquant, B
      BMC neuroscience  16  10  2015

      Show Abstract
      25880931 25880931
      Casein kinase 1 regulates sterol regulatory element-binding protein (SREBP) to control sterol homeostasis.
      Brookheart, RT; Lee, CY; Espenshade, PJ
      The Journal of biological chemistry  289  2725-35  2014

      Show Abstract
      24327658 24327658
      Inhibition of post-translational N-glycosylation by HRD1 that controls the fate of ABCG5/8 transporter.
      Suzuki, S; Shuto, T; Sato, T; Kaneko, M; Takada, T; Suico, MA; Cyr, DM; Suzuki, H; Kai, H
      Scientific reports  4  4258  2014

      Show Abstract
      24584735 24584735
      Novel mechanism of JNK pathway activation by adenoviral E1A.
      Romanov, VS; Brichkina, AI; Morrison, H; Pospelova, TV; Pospelov, VA; Herrlich, P
      Oncotarget  5  2176-86  2014

      Show Abstract
      24742962 24742962
      Kainate receptor post-translational modifications differentially regulate association with 4.1N to control activity-dependent receptor endocytosis.
      Copits, BA; Swanson, GT
      The Journal of biological chemistry  288  8952-65  2013

      Show Abstract
      23400781 23400781
      The deacetylase Sir2 from the yeast Clavispora lusitaniae lacks the evolutionarily conserved capacity to generate subtelomeric heterochromatin.
      Froyd, CA; Kapoor, S; Dietrich, F; Rusche, LN
      PLoS genetics  9  e1003935  2013

      Show Abstract
      24204326 24204326
      Distinct functional domains of the Abelson tyrosine kinase control axon guidance responses to Netrin and Slit to regulate the assembly of neural circuits.
      O'Donnell, MP; Bashaw, GJ
      Development (Cambridge, England)  140  2724-33  2013

      Show Abstract
      Immunoprecipitation23720041 23720041
      Mimicking p14ARF phosphorylation influences its ability to restrain cell proliferation.
      Vivo, M; Ranieri, M; Sansone, F; Santoriello, C; Calogero, RA; Calabrò, V; Pollice, A; La Mantia, G
      PloS one  8  e53631  2013

      Show Abstract
      23308265 23308265
      A role for CF1A 3' end processing complex in promoter-associated transcription.
      Al Husini, N; Kudla, P; Ansari, A
      PLoS genetics  9  e1003722  2013

      Show Abstract
      23966880 23966880

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