05-419 | Anti-Myc Tag Antibody, clone 9E10

05-419
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AIH(P), ICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-419
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Myc Tag Antibody, clone 9E10
      Background InformationEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
      The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
      References
      Product Information
      FormatPurified
      Control
      • Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.
      PresentationProtein G Purified immunoglobulin in 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-Myc Tag Antibody, clone 9E10 is an antibody against Myc Tag for use in IH(P), IC, IP & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 408-437 of the Leucine zipper region of human Myc (AEEQKLISEEDLLRKRREQLKHKLEQLRNS).
      Clone9E10
      HostMouse
      SpecificityRecognizes Myc and recombinant proteins containing the Myc epitope tag.
      IsotypeIgG1
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
      Gene Symbol
      • MYC
      • C-MYC
      • c-Myc
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
      SIZE: 439 amino acids; 48804 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated by PRKDC.
      DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular WeightPredicted MW for human Myc: 49 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on a Myc-tagged recombinant protein
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Myc Tag, clone 9E10 2475691
      Anti-Myc Tag, clone 9E10 - 2375574 2375574
      Anti-Myc Tag, clone 9E10 - 2393916 2393916
      Anti-Myc Tag, clone 9E10 - 2430481 2430481
      Anti-Myc Tag, clone 9E10 - 0703054296 0703054296
      Anti-Myc Tag, clone 9E10 - 15828 15828
      Anti-Myc Tag, clone 9E10 - 17827 17827
      Anti-Myc Tag, clone 9E10 - 18509 18509
      Anti-Myc Tag, clone 9E10 - 19162 19162
      Anti-Myc Tag, clone 9E10 - 1951973 1951973

      References

      Reference overviewApplicationPub Med ID
      Drug synergy drives conserved pathways to increase fission yeast lifespan.
      Huang, X; Leggas, M; Dickson, RC
      PloS one  10  e0121877  2015

      Show Abstract
      Western Blotting25786258 25786258
      Endocytic Adaptor Protein Tollip Inhibits Canonical Wnt Signaling.
      Toruń, A; Szymańska, E; Castanon, I; Wolińska-Nizioł, L; Bartosik, A; Jastrzębski, K; Miętkowska, M; González-Gaitán, M; Miaczynska, M
      PloS one  10  e0130818  2015

      Show Abstract
      26110841 26110841
      FANCI Regulates Recruitment of the FA Core Complex at Sites of DNA Damage Independently of FANCD2.
      Castella, M; Jacquemont, C; Thompson, EL; Yeo, JE; Cheung, RS; Huang, JW; Sobeck, A; Hendrickson, EA; Taniguchi, T
      PLoS genetics  11  e1005563  2015

      Show Abstract
      26430909 26430909
      A frameshift mutation in GRXCR2 causes recessively inherited hearing loss.
      Imtiaz, A; Kohrman, DC; Naz, S
      Human mutation  35  618-24  2014

      Show Abstract
      Immunofluorescence24619944 24619944
      The potential for isocitrate dehydrogenase mutations to produce 2-hydroxyglutarate depends on allele specificity and subcellular compartmentalization.
      Ward, PS; Lu, C; Cross, JR; Abdel-Wahab, O; Levine, RL; Schwartz, GK; Thompson, CB
      The Journal of biological chemistry  288  3804-15  2013

      Show Abstract
      23264629 23264629
      The yeast Snt2 protein coordinates the transcriptional response to hydrogen peroxide-mediated oxidative stress.
      Baker, LA; Ueberheide, BM; Dewell, S; Chait, BT; Zheng, D; Allis, CD
      Molecular and cellular biology  33  3735-48  2013

      Show Abstract
      Western Blotting23878396 23878396
      Production of a recombinant antibody specific for i blood group antigen, a mesenchymal stem cell marker.
      Hirvonen, T; Suila, H; Tiitinen, S; Natunen, S; Laukkanen, ML; Kotovuori, A; Reinman, M; Satomaa, T; Alfthan, K; Laitinen, S; Takkinen, K; Räbinä, J; Valmu, L
      BioResearch open access  2  336-45  2013

      Show Abstract
      24083089 24083089
      GRP78 overproduction in pancreatic beta cells protects against high-fat-diet-induced diabetes in mice.
      Teodoro-Morrison, T; Schuiki, I; Zhang, L; Belsham, DD; Volchuk, A
      Diabetologia  56  1057-67  2013

      Show Abstract
      23475366 23475366
      The histone chaperone Spt6 coordinates histone H3K27 demethylation and myogenesis.
      Wang, AH; Zare, H; Mousavi, K; Wang, C; Moravec, CE; Sirotkin, HI; Ge, K; Gutierrez-Cruz, G; Sartorelli, V
      The EMBO journal  32  1075-86  2013

      Show Abstract
      Western Blotting23503590 23503590
      Rpd3- and spt16-mediated nucleosome assembly and transcriptional regulation on yeast ribosomal DNA genes.
      Johnson, JM; French, SL; Osheim, YN; Li, M; Hall, L; Beyer, AL; Smith, JS
      Molecular and cellular biology  33  2748-59  2013

      Show Abstract
      23689130 23689130

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies