06-1029 | Anti-NET55 Antibody

06-1029
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1029
      DescriptionAnti-NET55 Antibody
      Alternate Names
      • anterior pharynx defective 1 homolog B (C. elegans)
      • presenilin stabilization factor-like
      • aph-1beta
      • gamma-secretase subunit APH-1B
      • anterior pharynx defective 1B-like
      • Presenilin-stabilization factor-like
      • Aph-1beta
      Background InformationNET55 is more commonly known as gamma-secretase subunit APH-1 beta (APH-1b). APH-1b is a multipass membrane protein that is related with nicastrin and the heterodimers of the presenilin fragments in human cell lines and rat brain. APH-1b is a part of the gamma-secretase enzyme compound which is responsible for neurodevelopmental signaling pathways.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Rat liver tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-NET55 Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect NET55 also known as anterior pharynx defective 1 homolog B (C. elegans) or gamma-secretase subunit APH-1B.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: 1:500 dilution from a representative lot detected NET55 in mouse brain tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human NET55.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAPH1 is a multipass transmembrane protein that interacts with presenilin (see PSEN1; MIM 104311) and nicastrin (APH2; MIM 605254) as a functional component of the gamma-secretase complex. The gamma-secretase complex is required for the intramembrane proteolysis of a number of membrane proteins, including the amyloid-beta precursor protein (APP; MIM 104760) and Notch (MIM 190198).[supplied by OMIM].
      Gene Symbol
      • PSFL
      • APH-1B
      • TAAV688
      • PRO13282
      • APH-1b
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Probably present in a minority of gamma-secretase complexes compared to APH1A.

      SUBUNIT STRUCTURE: Probable component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist (By similarity). Interacts with PSEN1 and PSEN2.

      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).

      TISSUE SPECIFICTY: Weakly or not expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, colon, skeletal muscle, heart and brain.

      SEQUENCE SIMILARITIES: Belongs to the APH-1 family.
      Molecular Weight ~31 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat liver tissue lysate.

      Western Blot Analysis: 2 µg/mL of this antibody detected NET55 on 10 µg of rat liver tissue lysate. Note: Western blot performed without Tween.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NET55 - NRG1830911 NRG1830911

      References

      Reference overviewPub Med ID
      Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery.
      Zuleger, N; Boyle, S; Kelly, DA; de las Heras, JI; Lazou, V; Korfali, N; Batrakou, DG; Randles, KN; Morris, GE; Harrison, DJ; Bickmore, WA; Schirmer, EC
      Genome biology  14  R14  2013

      Show Abstract
      23414781 23414781