04-804 | Anti-NFAT1 Antibody, clone 8G4.2

04-804
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-804
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-NFAT1 Antibody, clone 8G4.2
      Alternate Names
      • Nuclear factor of activated T-cells, cytoplasmic 2
      • NF-ATc2
      • NFATc2
      • NFAT pre-existing subunit
      • NF-Atp
      • T-cell transcription factor NFAT1
      Background InformationNFAT (Nuclear Factor of Activated T-cells) is a family of transcription factors that are located in the cytoplasm in their inactive state. NFAT translocates to the nucleus upon stimulation of the T-cell receptor where it plays a key role in gene expression in immune response. NFAT is dephosphorylated and activated by the phosphatase PP2B/Calcineurin. This results in its nuclear localization and transcriptional activity. For NFAT1 (NFATC2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2), calcineurin is known to target the region between residues 114-126. Mutations in this region of NFAT1 result in its inability to de-phosphorylate and subsequent nuclear translocation. Upon activation, NFAT mediates the transcriptional induction of cytokines and other immuno-regulatory genes during immune responses, and is expressed in T-cells, B-cells, mast cells and NK cells.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal in PBS with 0.05% NaN3.
      Applications
      ApplicationThis Anti-NFAT1 Antibody, clone 8G4.2 is validated for use in WB, IF for the detection of NFAT1.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Biological Information
      ImmunogenGST-fusion protein corresponding to amino acids 1-297 of human NFAT1.
      EpitopeN-term
      Clone8G4.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityDetects NFAT1.
      IsotypeIgMκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, and Rat. Other species have not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
      Gene Symbol
      • KIAA0611
      • NF-ATP
      • NF-ATp
      • NFAT1
      • NFATP
      • NFATp
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
      SUBUNIT: Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. The phosphorylated form specifically interacts with XPO1; which mediates nuclear export. NFATC proteins bind to DNA as monomers. Interacts with NFATC2IP (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC play a key role in the gene transcription.
      ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing. Additional isoforms seem to exist.
      TISSUE SPECIFICITY: Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
      INDUCTION: Inducibly expressed in T-lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin.
      DOMAIN: Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors (By similarity).
      PTM: In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
      SIMILARITY: Contains 1 RHD (Rel-like) domain.
      Molecular Weight120-140 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NFAT1, clone 8G4.2 - 2172755 2172755
      Anti-NFAT1, clone 8G4.2 - JH1769963 JH1769963
      Anti-NFAT1, clone 8G4.2 - NG1547146 NG1547146
      Anti-NFAT1, clone 8G4.2 - NG1631262 NG1631262
      Anti-NFAT1, clone 8G4.2 - NG1873194 NG1873194
      Anti-NFAT1, clone 8G4.2 -2499300 2499300
      Anti-NFAT1, clone 8G4.2 -2741546 2741546
      Anti-NFAT1, clone 8G4.2 -2748011 2748011
      Anti-NFAT1, clone 8G4.2 -2752545 2752545
      Anti-NFAT1, clone 8G4.2 -2788747 2788747

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies