06-418 | Anti-NFκB p65 Antibody, CT

06-418
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MEMSA, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-418
      Replaces04-1008; 04-235
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-NFκB p65 Antibody, CT
      Alternate Names
      • Nuclear factor NF-kappa-B p65 subunit
      • v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear
        factor of kappa light polypeptide gene enhancer in B-cells 3 (p65))
      • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
      • v-rel reticuloendotheliosis viral oncogene homolog A
      • nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
      • p65
      • v-rel reticuloendotheliosis viral oncogene homolog A
      • nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
      • p65 (avian)
      Background InformationThe transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.
      References
      Product Information
      FormatPurified
      Control
      • TNFα treated HeLa cells, PMA, A431 cells or calcium ionophore-treated Jurkat cells
        Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
      PresentationPurified rabbit polyclonal IgG in 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20°C.
      Applications
      ApplicationAnti-NFκB p65 Antibody, CT is a Rabbit Polyclonal Antibody for detection of NFκB p65 also known as Nuclear factor NF-kappa-B p65 subunit & has been validated in EMSA, IP & WB.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 µg of a previous lot immunoprecipitated NFκB p65 from 500 μg of a human A431 carcinoma cell lysate.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to the 13 C-terminal amino acids (C-DMDFSALLSQISS) of the p65 subunit of human NFκB containing an N-terminal cysteine for conjugation. The immunizing sequence is identical in mouse.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityNFκB p65, Mr 65 kDa
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
      Gene Symbol
      • RELA
      • MGC131774
      • NFKB3
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q04206 # p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF- kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex.
      SIZE: 551 amino acids; 60219 Da
      SUBUNIT: Active NF-kappa-B is a heterodimer of an about 50 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer. May interact with ETHE1. Binds AES and TLE1. Interacts with TP53BP2. Binds to and is phosphorylated by the activated form of either RPS6KA4 or RPS6KA5. Interacts with ING4 and this interaction may be indirect. Interacts with CARM1, USP48 and UNC5CL.
      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
      PTM: Ubiquitinated, leading to its proteosomal degradation. Degradation is required for termination of NF-kappa-B response.
      SIMILARITY: SwissProt: Q04206 ## Contains 1 RHD (Rel-like) domain.
      Molecular Weight65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on RIPA lysates from A431 cells.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected NFkB p65 in RIPA lysates from A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NF kB p65, CT - JBC1395528 JBC1395528
      Anti-NF#954;B p65, CT - 2123172 2123172
      Anti-NF#954;B p65, CT - DAM1774705 DAM1774705
      Anti-NFB p65, CT - 2040539 2040539
      Anti-NFB p65, CT - 2189944 2189944
      Anti-NFB p65, CT - 2203119 2203119
      Anti-NFB p65, CT - 2356106 2356106
      Anti-NFB p65, CT - DAM1687538 DAM1687538
      Anti-NFB p65, CT - DAM1713190 DAM1713190
      Anti-NFB p65, CT - DAM1745297 DAM1745297

      References

      Reference overviewApplicationPub Med ID
      Calcitriol ameliorates renal damage in a pre-established proteinuria model.
      Maquigussa, E; Arnoni, CP; Pereira, LG; Boim, MA
      Molecular medicine reports  12  1009-15  2015

      Show Abstract
      25823676 25823676
      An activated unfolded protein response promotes retinal degeneration and triggers an inflammatory response in the mouse retina.
      Rana, T; Shinde, VM; Starr, CR; Kruglov, AA; Boitet, ER; Kotla, P; Zolotukhin, S; Gross, AK; Gorbatyuk, MS
      Cell death & disease  5  e1578  2014

      Show Abstract
      25522272 25522272
      NF-κB plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment.
      Huang, G; Wen, Q; Zhao, Y; Gao, Q; Bai, Y
      PloS one  8  e61602  2013

      Show Abstract
      23585913 23585913
      SIMPL enhancement of tumor necrosis factor-α dependent p65-MED1 complex formation is required for mammalian hematopoietic stem and progenitor cell function.
      Zhao, W; Breese, E; Bowers, A; Hoggatt, J; Pelus, LM; Broxmeyer, HE; Goebl, M; Harrington, MA
      PloS one  8  e61123  2013

      Show Abstract
      23630580 23630580
      The response of human macrophages to β-glucans depends on the inflammatory milieu.
      Municio, C; Alvarez, Y; Montero, O; Hugo, E; Rodríguez, M; Domingo, E; Alonso, S; Fernández, N; Crespo, MS
      PloS one  8  e62016  2013

      Show Abstract
      Western Blotting23637950 23637950
      miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells.
      Hsieh, JY; Huang, TS; Cheng, SM; Lin, WS; Tsai, TN; Lee, OK; Wang, HW
      Nucleic acids research  41  9753-63  2013

      Show Abstract
      Western Blotting23963696 23963696
      BCL-3 attenuation of TNFA expression involves an incoherent feed-forward loop regulated by chromatin structure.
      Walker, T; Adamson, A; Jackson, DA
      PloS one  8  e77015  2013

      Show Abstract
      24130828 24130828
      Inhibition of endoplasmic reticulum stress alleviates lipopolysaccharide-induced lung inflammation through modulation of NF-κB/HIF-1α signaling pathway.
      Kim, HJ; Jeong, JS; Kim, SR; Park, SY; Chae, HJ; Lee, YC
      Scientific reports  3  1142  2013

      Show Abstract
      23359618 23359618
      Sirtuin 1 is a key regulator of the interleukin-12 p70/interleukin-23 balance in human dendritic cells.
      Alvarez, Y; Rodríguez, M; Municio, C; Hugo, E; Alonso, S; Ibarrola, N; Fernández, N; Crespo, MS
      The Journal of biological chemistry  287  35689-701  2012

      Show Abstract
      22893703 22893703
      Myh7b/miR-499 gene expression is transcriptionally regulated by MRFs and Eos.
      Yeung, F; Chung, E; Guess, MG; Bell, ML; Leinwand, LA
      Nucleic acids research  40  7303-18  2012

      Show Abstract
      22638570 22638570

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies