05-710 | Anti-NG2 Antibody, clone 132.38

05-710
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RIHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-710
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-NG2 Antibody, clone 132.38
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationProtein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative.
      Applications
      ApplicationAnti-NG2 Antibody, clone 132.38 detects level of NG2 & has been published & validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesCross-reactivity expected with mouse.
      Biological Information
      ImmunogenHEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222
      Clone132.38
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes NG2.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryA human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
      Gene Symbol
      • CSPG4
      • MCSP
      • HMW-MAA
      • MCSPG
      • MSK16
      • NG2
      • MEL-CSPG
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.


      SIZE: 2322 amino acids; 250497 Da

      SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.

      SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).

      TISSUE SPECIFICITY: Detected only in malignant melanoma cells.



      PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).


      SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.

      MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.
      Molecular Weight~270-300 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on whole rat brain preparation
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NG2, clone 132.38 2475728
      Anti-NG2, clone 132.38 (mouse monoclonal IgG1) - 2450197 2450197
      Anti-NG2, clone 132.38 (mouse monoclonal IgG1) 2796319
      Anti-NG2, clone 132.38 (mouse monoclonal IgG1) 2901516
      Anti-NG2, clone 132.38 (mouse monoclonal IgG1) - 2250527 2250527
      Anti-NG2, clone 132.38 - 0701049378 0701049378
      Anti-NG2, clone 132.38 - 2020328 2020328
      Anti-NG2, clone 132.38 - 24437 24437
      Anti-NG2, clone 132.38 - JBC1942568 JBC1942568
      Anti-NG2, clone 132.38 -2607257 2607257

      References

      Reference overviewApplicationSpeciesPub Med ID
      Histone deacetylase expression in white matter oligodendrocytes after stroke.
      Kassis, H; Chopp, M; Liu, XS; Shehadah, A; Roberts, C; Zhang, ZG
      Neurochemistry international  77  17-23  2014

      Show Abstract
      24657831 24657831
      Collagen signaling enhances tumor progression after anti-VEGF therapy in a murine model of pancreatic ductal adenocarcinoma.
      Aguilera, KY; Rivera, LB; Hur, H; Carbon, JG; Toombs, JE; Goldstein, CD; Dellinger, MT; Castrillon, DH; Brekken, RA
      Cancer research  74  1032-44  2014

      Show Abstract
      24346431 24346431
      The expression of spinal methyl-CpG-binding protein 2, DNA methyltransferases and histone deacetylases is modulated in persistent pain states.
      Tochiki, KK; Cunningham, J; Hunt, SP; Géranton, SM
      Molecular pain  8  14  2012

      Show Abstract
      22369085 22369085
      Sensory and cortical activation of distinct glial cell subtypes in the somatosensory thalamus of young rats.
      H Rheinallt Parri,Timothy M Gould,Vincenzo Crunelli
      The European journal of neuroscience  32  2010

      Show Abstract Full Text Article
      20608967 20608967
      In vivo intracellular recording suggests that gray matter astrocytes in mature cerebral cortex and hippocampus are electrophysiologically homogeneous.
      Mishima, T; Hirase, H
      The Journal of neuroscience : the official journal of the Society for Neuroscience  30  3093-100  2010

      Show Abstract
      Immunohistochemistry20181606 20181606
      JAM-A is a novel surface marker for NG2-Glia in the adult mouse brain.
      Stelzer, Sandra, et al.
      BMC Neurosci, 11: 27 (2010)  2010

      Show Abstract
      20184779 20184779
      A powerful transgenic tool for fate mapping and functional analysis of newly generated neurons.
      Zhang, J; Giesert, F; Kloos, K; Vogt Weisenhorn, DM; Aigner, L; Wurst, W; Couillard-Despres, S
      BMC neuroscience  11  158  2010

      Show Abstract
      Immunohistochemistry21194452 21194452
      Genesis of neuronal and glial progenitors in the cerebellar cortex of peripuberal and adult rabbits.
      Ponti, G; Peretto, P; Bonfanti, L
      PloS one  3  e2366  2008

      Show Abstract
      Rabbit18523645 18523645
      Multiple regions of the NG2 proteoglycan inhibit neurite growth and induce growth cone collapse
      Ughrin, Yvonne M, et al
      J Neurosci, 23:175-86 (2003)  2003

      Immunohistochemistry (Tissue)Rat12514214 12514214
      The oligodendrocyte precursor cell in health and disease
      Levine, J M, et al
      Trends Neurosci, 24:39-47 (2001)  2001

      11163886 11163886

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies