05-432 | Anti-NR1 Antibody, CT

05-432
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-432
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-NR1 Antibody, CT
      Alternate Names
      • Grin1
      • NMDA R1 receptor C1 cassette
      • glutamate receptor, ionotropic
      • N-methyl D-aspartate 1
      Background InformationNR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteriztic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationPurified mouse monoclonal IgG in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
      Applications
      ApplicationAnti-NR1 Antibody, CT detects level of NR1 & has been published & validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μg of a previous lot immunoprecipitated NR1 from 500mg of rat brain microsomal protein preparation.

      Immunocytochemistry:
      An independent laboratory has shown positive staining in QT-6 cells, transfected to express NR1, which were fixed with 4% paraformaldehyde/4% sucrose in PBS and in nontransfected cultured rat neurons.

      Note: Do not boil the microsomal preparation. Incubate at room temperature for 30-45 minutes.
      Biological Information
      ImmunogenHis-tag fusion protein corresponding to residues 834-938 of rat NR1A. The immunizing sequence is identical in rat and human.
      EpitopeThe epitope maps to a region (a.a. 834-864) common to all NR1 isoforms
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody is specific for NR1, Mr 130 kDa. The epitope maps to a region (834-864) common to all NR1 isoforms and so should cross react with all NR1 isoforms. An additional band may be detected at higher concentrations, Mr 200kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteRat. Immunoreactivity is predicted for human and mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NR1
      • Grin1
      • Nmdar1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.

      SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein, Cell junction › synapse › postsynaptic cell membrane. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Note: Enriched in post-synaptic plasma membrane and post-synaptic densities.

      POST-TRANSLATIONAL MODIFICATION: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.

      SIZE: 938 amino acids; 105,509 Da
      Molecular Weight130 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on rat brain microsomal protein preparation.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected NR1 from 20 μg of rat brain microsomal protein preparation (Catalog # 12-144).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2° to 8ºC from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-NR1, CT (mouse monoclonal IgG) - DAM1413902 DAM1413902
      Anti-NR1, CT - 2383521 2383521
      Anti-NR1, CT - 2455626 2455626
      Anti-NR1, CT - 17443 17443
      Anti-NR1, CT - 1988741 1988741
      Anti-NR1, CT - 2041025 2041025
      Anti-NR1, CT - 21353 21353
      Anti-NR1, CT - 2167783 2167783
      Anti-NR1, CT - 21961 21961
      Anti-NR1, CT - 2278508 2278508

      References

      Reference overviewApplicationSpeciesPub Med ID
      STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs.
      Garcia-Alvarez, G; Lu, B; Yap, KA; Wong, LC; Thevathasan, JV; Lim, L; Ji, F; Tan, KW; Mancuso, JJ; Tang, W; Poon, SY; Augustine, GJ; Fivaz, M
      Molecular biology of the cell  26  1141-59  2015

      Show Abstract
      25609091 25609091
      A naturally occurring null variant of the NMDA type glutamate receptor NR3B subunit is a risk factor of schizophrenia.
      Matsuno, H; Ohi, K; Hashimoto, R; Yamamori, H; Yasuda, Y; Fujimoto, M; Yano-Umeda, S; Saneyoshi, T; Takeda, M; Hayashi, Y
      PloS one  10  e0116319  2015

      Show Abstract
      Western Blotting25768306 25768306
      DYRK1A-mediated phosphorylation of GluN2A at Ser(1048) regulates the surface expression and channel activity of GluN1/GluN2A receptors.
      Grau, C; Arató, K; Fernández-Fernández, JM; Valderrama, A; Sindreu, C; Fillat, C; Ferrer, I; de la Luna, S; Altafaj, X
      Frontiers in cellular neuroscience  8  331  2014

      Show Abstract
      Immunoprecipitation25368549 25368549
      Simvastatin treatment enhances NMDAR-mediated synaptic transmission by upregulating the surface distribution of the GluN2B subunit.
      Parent, MA; Hottman, DA; Cheng, S; Zhang, W; McMahon, LL; Yuan, LL; Li, L
      Cellular and molecular neurobiology  34  693-705  2014

      Show Abstract
      24687455 24687455
      Effect of D-cycloserine in conjunction with fear extinction training on extracellular signal-regulated kinase activation in the medial prefrontal cortex and amygdala in rat.
      Gupta, SC; Hillman, BG; Prakash, A; Ugale, RR; Stairs, DJ; Dravid, SM
      The European journal of neuroscience  37  1811-22  2013

      Show Abstract
      Western Blotting23551217 23551217
      RNF38 encodes a nuclear ubiquitin protein ligase that modifies p53.
      Sheren, JE; Kassenbrock, CK
      Biochemical and biophysical research communications  440  473-8  2013

      Show Abstract
      23973461 23973461
      Synaptic alterations in the rTg4510 mouse model of tauopathy.
      Kopeikina, KJ; Polydoro, M; Tai, HC; Yaeger, E; Carlson, GA; Pitstick, R; Hyman, BT; Spires-Jones, TL
      The Journal of comparative neurology  521  1334-53  2013

      Show Abstract
      Mouse23047530 23047530
      Dab1 is required for synaptic plasticity and associative learning.
      Trotter, J; Lee, GH; Kazdoba, TM; Crowell, B; Domogauer, J; Mahoney, HM; Franco, SJ; Müller, U; Weeber, EJ; D'Arcangelo, G
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  15652-68  2013

      Show Abstract
      Immunofluorescence24068831 24068831
      Enhanced expression of NR2B subunits of NMDA receptors in the inherited glaucomatous DBA/2J mouse retina.
      Dong, LD; Chen, J; Li, F; Gao, F; Wu, J; Miao, Y; Wang, Z
      Neural plasticity  2013  670254  2013

      Show Abstract
      24175101 24175101
      Glutamate binding to the GluN2B subunit controls surface trafficking of N-methyl-D-aspartate (NMDA) receptors.
      She, K; Ferreira, JS; Carvalho, AL; Craig, AM
      The Journal of biological chemistry  287  27432-45  2012

      Show Abstract
      22740692 22740692

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies