05-382 | Anti-Na+/K+ ATPase β-1 Antibody, clone C464.8

05-382
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ca, H, R, RbIHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-382
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Na+/K+ ATPase β-1 Antibody, clone C464.8
      OverviewClone C464.8 is also referred to as clone 8A
      References
      Product Information
      FormatPurified
      Control
      • Expressed in most tissue, including brain, heart and kidney
      PresentationProtein G Purified immunoglobulin in Protein G Purified immunoglobulin in PBS with 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationThis Anti-Na+/K+ ATPase β-1 Antibody, clone C464.8 is validated for use in IH, WB for the detection of Na+/K+ ATPase β-1.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Biological Information
      ImmunogenPurified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
      CloneC464.8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes the β-1 subunit isoform of Na+/K+.
      IsotypeIgG2aκ
      Species Reactivity
      • Bovine
      • Canine
      • Human
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit.
      Gene Symbol
      • ATP1A3
      • MGC13276
      • RDP
      • IIIsubunit).
      • DYT12
      • +K
      • Na
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13637 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
      SIZE: 1013 amino acids; 111749 Da
      SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      DISEASE: SwissProt: P13637 # Defects in ATP1A3 are the cause of dystonia-12 (DYT12) [MIM:128235]; also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant form of dystonia- parkinsonism characterized by an unusually rapid evolution of signs and symptoms. Affected persons develop dystonia and parkinsonism between 14 and 45 years of age. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability.
      SIMILARITY: SwissProt: P13637 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.
      Molecular Weightbroad band at an apparent molecular weight of 48-55 kDa due to glycosylation
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated in immunoblot on rat brain, rat heart or rat kidney microsomal preparations
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Na+/K+ ATPase, #61538;-1, clone C464.8 (mouse monoclonal IgG2a#61547;) - 2018110 2018110
      Anti-Na+/K+ ATPase, B-1, clone C464.8 (mouse monoclonal IgG2aK) 2944579
      Anti-Na+/K+ ATPase, b-1, clone C464.8 (mouse monoclonal IgG2ak) - 1962656 1962656
      Anti-Na+/K+ ATPase, b-1, clone C464.8 (mouse monoclonal IgG2ak) - 2262768 2262768
      Anti-Na+/K+ ATPase, b-1, clone C464.8 - DAM1413899 DAM1413899
      Anti-Na+/K+ ATPase, b-1, clone C464.8 - DAM1731465 DAM1731465
      Anti-Na+/K+ ATPase, b-1, clone C464.8 - JBC1949766 JBC1949766
      Anti-Na+/K+ ATPase, ß-1, clone C464.8 -2834665 2834665
      Anti-Na+/K+ ATPase, β-1, clone C464.8 (mouse monoclonal IgG2aκ) -2502986 2502986
      Anti-Na+/K+ ATPase, β-1, clone C464.8 -2685213 2685213

      References

      Reference overviewApplicationPub Med ID
      Apical sorting of lysoGPI-anchored proteins occurs independent of association with detergent-resistant membranes but dependent on their N-glycosylation.
      Castillon, GA; Michon, L; Watanabe, R
      Molecular biology of the cell  24  2021-33  2013

      Show Abstract
      23615438 23615438
      Motor neuron degeneration in amyotrophic lateral sclerosis mutant superoxide dismutase-1 transgenic mice: mechanisms of mitochondriopathy and cell death.
      Lee J Martin,Zhiping Liu,Kevin Chen,Ann C Price,Yan Pan,Jason A Swaby,W Christopher Golden
      The Journal of comparative neurology  500  2007

      Show Abstract
      17099894 17099894
      Regional distribution of Na,K-ATPase activity in porcine lens epithelium
      Tamiya, S., et al
      Invest Ophthalmol Vis Sci, 44:4395-9 (2003)  2003

      Immunoblotting (Western)14507885 14507885
      Thyroid hormone stimulates Na-K-ATPase activity and its plasma membrane insertion in rat alveolar epithelial cells.
      Jianxun Lei, Sogol Nowbar, Cary N Mariash, David H Ingbar
      American journal of physiology. Lung cellular and molecular physiology  285  L762-72  2003

      Show Abstract
      12740220 12740220
      Expression and molecular regulation of Na(+)-K(+)-ATPase after renal ischemia.
      Van Why, S K, et al.
      Am. J. Physiol., 267: F75-85 (1994)  1994

      Show Abstract
      8048568 8048568
      Analysis of subunit assembly of the Na-K-ATPase.
      Fambrough, D M, et al.
      Am. J. Physiol., 266: C579-89 (1994)  1994

      Show Abstract
      8166221 8166221
      The alpha and beta subunits of the Na,K-ATPase can assemble at the plasma membrane into functional enzyme.
      A W DeTomaso, G Blanco, R W Mercer
      The Journal of cell biology  127  55-69  1994

      Show Abstract Full Text Article
      7929571 7929571
      An ion-transporting ATPase encodes multiple apical localization signals.
      Gottardi, C J and Caplan, M J
      J. Cell Biol., 121: 283-93 (1993)  1993

      Show Abstract
      8385670 8385670
      Delivery of Na+,K(+)-ATPase in polarized epithelial cells.
      Gottardi, C J and Caplan, M J
      Science, 260: 552-4; author reply 554-6 (1993)  1993

      8386395 8386395
      Molecular requirements for the cell-surface expression of multisubunit ion-transporting ATPases. Identification of protein domains that participate in Na,K-ATPase and H,K-ATPase subunit assembly.
      Gottardi, C J and Caplan, M J
      J. Biol. Chem., 268: 14342-7 (1993)  1993

      Show Abstract
      8390991 8390991

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies