06-288 | Anti-Nck Antibody

100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBRbPurifiedPolyclonal Antibody
      Catalogue Number06-288
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Nck Antibody
      Product Information
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing 0.05% sodium azide
      ApplicationDetect Nck using this Anti-Nck Antibody validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenPurified GST fusion protein produced in E. coli., corresponding to human Nck amino acid sequence 1 to 254
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS.
      Gene Symbol
      • NCK1
      • MGC12668
      • NCK
      • NCKalpha
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16333 # Adapter protein which associates with tyrosine- phosphorylated growth factor receptors or their cellular substrates.
      SIZE: 377 amino acids; 42864 Da
      SUBUNIT: Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with SOCS7.
      PTM: Phosphorylated on Ser and Tyr residues.
      SIMILARITY: SwissProt: P16333 ## Contains 1 SH2 domain. & Contains 3 SH3 domains.
      Molecular Weight47kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on human A431 carcinoma cells, mouse 3T3/A31 fibroblasts or rat L6 skeletal fibroblasts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Nck (rabbit polyclonal IgG) - 2462833 2462833
      Anti-Nck (rabbit polyclonal IgG) - 2168330 2168330
      Anti-Nck (rabbit polyclonal IgG) -2489004 2489004
      Anti-Nck - 13782 13782
      Anti-Nck - 2032292 2032292
      Anti-Nck - 2054516 2054516
      Anti-Nck - 24143 24143
      Anti-Nck - 24143A 24143A
      Anti-Nck - DAM1687443 DAM1687443
      Anti-Nck - JBC1940051 JBC1940051


      Reference overviewApplicationPub Med ID
      Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation.
      Humphries, AC; Donnelly, SK; Way, M
      Journal of cell science  127  673-85  2014

      Show Abstract
      24284073 24284073
      Coordinated activation of the Rac-GAP β2-chimaerin by an atypical proline-rich domain and diacylglycerol.
      Gutierrez-Uzquiza, A; Colon-Gonzalez, F; Leonard, TA; Canagarajah, BJ; Wang, H; Mayer, BJ; Hurley, JH; Kazanietz, MG
      Nature communications  4  1849  2013

      Show Abstract
      23673634 23673634
      Complex formation of EphB1/Nck/Caskin1 leads to tyrosine phosphorylation and structural changes of the Caskin1 SH3 domain.
      Pesti, S; Balázs, A; Udupa, R; Szabó, B; Fekete, A; Bőgel, G; Buday, L
      Cell communication and signaling : CCS  10  36  2012

      Show Abstract
      23181695 23181695
      F11-mediated inhibition of RhoA signalling enhances the spread of vaccinia virus in vitro and in vivo in an intranasal mouse model of infection.
      João V Cordeiro,Susana Guerra,Yoshiki Arakawa,Mark P Dodding,Mariano Esteban,Michael Way
      PloS one  4  2009

      Show Abstract Full Text Article
      20041165 20041165
      p21-activated kinase 1 (PAK1) interacts with the Grb2 adapter protein to couple to growth factor signaling.
      Lorena A Puto, Kersi Pestonjamasp, Charles C King, Gary M Bokoch, Lorena A Puto, Kersi Pestonjamasp, Charles C King, Gary M Bokoch
      The Journal of biological chemistry  278  9388-93  2003

      Show Abstract
      Immunoblotting (Western)12522133 12522133
      Role of Dok1 in cell signaling mediated by RET tyrosine kinase
      Murakami, H., et al
      J Biol Chem, 277:32781-90 (2002)  2002

      Immunoblotting (Western)12087092 12087092
      Src homology region 2 domain-containing phosphatase 1 positively regulates B cell receptor-induced apoptosis by modulating association of B cell linker protein with Nck and activation of c-Jun NH2-terminal kinase.
      Mizuno, Kazuya, et al.
      J. Immunol., 169: 778-86 (2002)  2002

      Show Abstract
      Immunoblotting (Western)12097380 12097380
      The adaptor protein Nck-1 couples the netrin-1 receptor DCC (deleted in colorectal cancer) to the activation of the small GTPase Rac1 through an atypical mechanism
      Li, X., et al
      J Biol Chem, 277:37788-97 (2002)  2002

      Immunoblotting (Western)12149262 12149262
      Angiotensin II-induced stimulation of p21-activated kinase and c-Jun NH2-terminal kinase is mediated by Rac1 and Nck.
      Schmitz, U; Thömmes, K; Beier, I; Wagner, W; Sachinidis, A; Düsing, R; Vetter, H
      The Journal of biological chemistry  276  22003-10  2001

      Show Abstract
      Immunoblotting (Western)11279250 11279250
      Independent SH2-binding sites mediate interaction of Dok-related protein with RasGTPase-activating protein and Nck
      Lock, P., et al
      J Biol Chem, 274:22775-84 (1999)  1999

      Immunoblotting (Western)10428862 10428862

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      Life Science Research > Antibodies and Assays > Primary Antibodies