05-565 | Anti-Nucleolin Antibody, clone 3G4B2

05-565
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, MkIP, WB, ICCMCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number05-565
      ReplacesMABE466
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Nucleolin Antibody, clone 3G4B2
      Background InformationNucleolin, also known as C23, is the major nucleolar protein of exponentially growing eukaryotic cells. It is a ubiquitous conserved multi-domain phosphoprotein found in all vertebrate species. Nucleolin contains a specific bipartite nuclear localization signal sequence and three different structural domains. Nucleolin is a multifunctional protein involved in most steps of ribosome biogenesis. Nucleolin has been implicated in chromatin structure, rDNA transcription, rRNA maturation, ribosome assembly, cytokinesis, nucleogenesis, cell proliferation and growth, and
      nucleo-cytoplasmic transport. Nucleolin also exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation, and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2, and protein kinase C-ζ.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • HeLa nuclear extract
      Presentationmouse culture supernatant containing 0.05% sodium azide prior to the addition of glycerol to 30%
      Applications
      ApplicationAnti-Nucleolin Antibody, clone 3G4B2 is a high quality Mouse Monoclonal Antibody for the detection of Nucleolin & has been validated in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenNuclear matrix extract from MDCK cells
      Cloneclone 3G4B2
      HostMouse
      Specificitynucleolin
      IsotypeIgG
      Species Reactivity
      • Canine
      • Human
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
      Gene Symbol
      • NCL
      • FLJ45706
      • nucleolin
      • C23
      • Nucleolin
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P19338 # Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation.
      SIZE: 710 amino acids; 76614 Da
      SUBUNIT: Interacts with APTX and NSUN2. Component of the SWAP complex that consists of NPM1, NCL/nucleolin, PARP1 and SWAP70. Component of a complex which is at least composed of HTATSF1/Tat- SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin.
      SUBCELLULAR LOCATION: Nucleus, nucleolus.
      SIMILARITY: SwissProt: P19338 ## Contains 4 RRM (RNA recognition motif) domains.
      Molecular Weight100kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Nucleolin, clone 3G4B2 2475726
      Anti-Nucleolin, clone 3G4B2 (culture supernatant, IgG1k) 2920193
      Anti-Nucleolin, clone 3G4B2 - 19894 19894
      Anti-Nucleolin, clone 3G4B2 - 25920 25920
      Anti-Nucleolin, clone 3G4B2 - 27818 27818

      References

      Reference overviewApplicationPub Med ID
      Experimental characterization of the human non-sequence-specific nucleic acid interactome.
      Dürnberger, G; Bürckstümmer, T; Huber, K; Giambruno, R; Doerks, T; Karayel, E; Burkard, TR; Kaupe, I; Müller, AC; Schönegger, A; Ecker, GF; Lohninger, H; Bork, P; Bennett, KL; Superti-Furga, G; Colinge, J
      Genome biology  14  R81  2013

      Show Abstract
      23902751 23902751
      Nucleophosmin mutations alter its nucleolar localization by impairing G-quadruplex binding at ribosomal DNA.
      Chiarella, S; De Cola, A; Scaglione, GL; Carletti, E; Graziano, V; Barcaroli, D; Lo Sterzo, C; Di Matteo, A; Di Ilio, C; Falini, B; Arcovito, A; De Laurenzi, V; Federici, L
      Nucleic acids research  41  3228-39  2013

      Show Abstract
      23328624 23328624
      The PI3K/Akt/mTOR pathway is implicated in the premature senescence of primary human endothelial cells exposed to chronic radiation.
      Yentrapalli, R; Azimzadeh, O; Sriharshan, A; Malinowsky, K; Merl, J; Wojcik, A; Harms-Ringdahl, M; Atkinson, MJ; Becker, KF; Haghdoost, S; Tapio, S
      PloS one  8  e70024  2013

      Show Abstract
      ELISA23936371 23936371
      Interplay between REST and nucleolin transcription factors: a key mechanism in the overexpression of genes upon increased phosphorylation.
      Tediose, Teeo, et al.
      Nucleic acids research, (2010)  2010

      Show Abstract
      20100803 20100803
      Developmental expression of pluripotency determining factors in caprine embryos: novel pattern of NANOG protein localization in the nucleolus.
      Shuyang He, Disha Pant, Andrew Schiffmacher, Steve Bischoff, David Melican, William Gavin, Carol Keefer
      Molecular reproduction and development  73  1512-22  2006

      Show Abstract
      16894532 16894532
      Dynamic relocalization of hOGG1 during the cell cycle is disrupted in cells harbouring the hOGG1-Cys326 polymorphic variant
      Luna, Luisa, et al.
      Nucleic Acids Res, 33:1813-1824 (2005)  2005

      15800211 15800211
      Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells
      Legrand, Dominique, et al
      Eur J Biochem, 271:303-317 (2004)  2004

      14717698 14717698
      Nonphosphorylated human La antigen interacts with nucleolin at nucleolar sites involved in rRNA biogenesis
      Intine, Robert V, et al.
      Mol Cell Biol, 24:10894-10904 (2004)  2004

      15572691 15572691
      Mitotic-like Tau Phosphorylation by p25-Cdk5 Kinase Complex
      Hamdane, M., et al
      J Biol Chem, 278:34026-34 (2003)  2003

      Immunoblotting (Western)12826674 12826674
      Human DNA glycosylases of the bacterial Fpg/MutM superfamily: an alternative pathway for the repair of 8-oxoguanine and other oxidation products in DNA
      Morland, Ingrid, et al
      Nucleic Acids Res, 30:4926-36 (2002)  2002

      12433996 12433996

      Brochure

      Title
      RNA-Binding Protein Immunoprecipitation

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      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies