05-905 | Anti-Nuf2 Antibody, clone 28-37

200 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WB, ICCMPurifiedMonoclonal Antibody
      Catalogue Number05-905
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Nuf2 Antibody, clone 28-37
      OverviewNuf2 is a protein part of a complex of kinetochore proteins that serve to attach microtubules to the kinetochore. Also called CDCA1. Members of the Ndc80/Nuf2 complex have been shown in several systems to be important in formation of stable kinetochore-microtubule attachments and chromosome alignment in mitosis. In HeLa cells, it has been shown that depletion of Nuf2 by RNAi results in a strong prometaphase block with an active spindle checkpoint. A smaller 320 AA splice variant would not be recognized by this ab.
      Product Information
      ApplicationAnti-Nuf2 Antibody, clone 28-37 is a high quality Mouse Monoclonal Antibody for the detection of Nuf2 & has been validated in IP, WB, ICC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenHis-tagged fusion protein corresponding to full length human Nuf2
      Cloneclone 28-37
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.
      Gene Symbol
      • NUF2
      • hNuf2R
      • NUF2R
      • hsNuf2
      • CDCA1
      • hNuf2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9BZD4 # Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
      SIZE: 464 amino acids; 54304 Da
      SUBUNIT: Component of the NDC80 complex, which consists of KNTC2/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of KNTC2/HEC1-CDCA1 and SPBC24- SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C- terminal regions of both subunits of the KNTC2/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B.
      SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.
      PTM: Can be phosphorylated by AURKA and AURKB.
      SIMILARITY: SwissProt: Q9BZD4 ## Belongs to the NUF2 family.
      Molecular WeightMr 53kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from acid extracted HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditionsstable 2 years at -20°C from date of shipment
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information


      Certificates of Analysis

      TitleLot Number
      Anti-Nuf2, clone 28-37 - 30385 30385


      Reference overviewPub Med ID
      Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1.
      Stucke, Volker M, et al.
      Chromosoma, 113: 1-15 (2004)  2004

      Show Abstract
      15235793 15235793

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      Life Science Research > Antibodies and Assays > Primary Antibodies