05-347 | Anti-PCNA Antibody, clone PC10

05-347
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtWB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-347
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PCNA Antibody, clone PC10
      Background InformationExpression of proliferating cell nuclear antigen (PCNA), cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Anti-PCNA reacts with PCNA at the molecular weight of 36 kDa on western blot.
      References
      Product Information
      FormatPurified
      Control
      • Rat kidney, human tonsil tissue
      PresentationProtein A purified mouse immunoglobulin in PBS containing 1 mg/mL BSA and 10mM Sodium Azide.
      Applications
      ApplicationDetect PCNA with Anti-PCNA Antibody, clone PC10 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC & IHC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Biological Information
      ImmunogenRecombinant rat PCNA
      Cloneclone PC10
      HostMouse
      SpecificityPCNA
      IsotypeIgG2aκ
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
      Gene Symbol
      • PCNA
      • Cyclin
      • cyclin
      • MGC8367
      Purification MethodConcentrated culture supernatant
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12004 # This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
      SIZE: 261 amino acids; 28769 Da
      SUBUNIT: Homotrimer. Interacts with KCTD10 (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Upon methyl methanesulfonate-induced DNA damage, mono- ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligase SHPRH, whih is required for DNA repair.
      DISEASE:SwissProt: P12004 # Antibodies are present in sera from patients with systemic lupus erythematosus.
      SIMILARITY: SwissProt: P12004 ## Belongs to the PCNA family.
      Molecular Weight36kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysate from human A431 cells or mouse 3T3/A31 cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PCNA, clone PC10 (mouse monoclonal IgG2ak) - 2344661 2344661
      Anti-PCNA, clone PC10 - 16153 16153
      Anti-PCNA, clone PC10 - 17254 17254
      Anti-PCNA, clone PC10 - 20764 20764
      Anti-PCNA, clone PC10 - 22928 22928
      Anti-PCNA, clone PC10 - 26557 26557
      Anti-PCNA, clone PC10 - 32555 32555
      Anti-PCNA, clone PC10 - JBC1370787 JBC1370787

      References

      Reference overviewApplicationPub Med ID
      Foxa2 acts as a co-activator potentiating expression of the Nurr1-induced DA phenotype via epigenetic regulation.
      Yi, SH; He, XB; Rhee, YH; Park, CH; Takizawa, T; Nakashima, K; Lee, SH
      Development (Cambridge, England)  141  761-72  2014

      Show Abstract
      Immunofluorescence24496614 24496614
      DNA methyltransferase 3b preferentially associates with condensed chromatin.
      Kashiwagi, K; Nimura, K; Ura, K; Kaneda, Y
      Nucleic acids research  39  874-88  2011

      Show Abstract
      Western Blotting20923784 20923784
      Fgfr2 is required for the development of the medial prefrontal cortex and its connections with limbic circuits.
      Stevens, HE; Smith, KM; Maragnoli, ME; Fagel, D; Borok, E; Shanabrough, M; Horvath, TL; Vaccarino, FM
      The Journal of neuroscience : the official journal of the Society for Neuroscience  30  5590-602  2010

      Show Abstract Full Text Article
      20410112 20410112
      Lsh, a modulator of CpG methylation, is crucial for normal histone methylation.
      Yan, Q; Huang, J; Fan, T; Zhu, H; Muegge, K
      The EMBO journal  22  5154-62  2003

      Show Abstract
      Western Blotting14517253 14517253
      Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay
      Shibata, Y. and Nakamura, T.
      J Biol Chem, 277:746-54 (2002)  2002

      Immunoblotting (Western)11687589 11687589
      Adenosine reverses a preestablished CCl4-induced micronodular cirrhosis through enhancing collagenolytic activity and stimulating hepatocyte cell proliferation in rats
      Hernandez-Munoz, R., et al
      Hepatology, 34:677-87 (2001)  2001

      Immunoblotting (Western)11584363 11584363
      Proliferating cell nuclear antigen is required for DNA excision repair.
      Shivji, K K, et al.
      Cell, 69: 367-74 (1992)  1992

      Show Abstract
      1348971 1348971
      Mechanism of elongation of primed DNA by DNA polymerase delta, proliferating cell nuclear antigen, and activator 1.
      Lee, S H and Hurwitz, J
      Proc. Natl. Acad. Sci. U.S.A., 87: 5672-6 (1990)  1990

      Show Abstract
      1974050 1974050
      Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
      Waseem, N H and Lane, D P
      J. Cell. Sci., 96 ( Pt 1): 121-9 (1990)  1990

      Show Abstract
      1695635 1695635

      FAQ

      QuestionAnswer
      I would like to use this monoclonal antibody on mouse tissue, are there any special procedures I should follow?To reduce the background interaction between the monoclonal antibody and the mouse tissue, you will need to perform antigen retrieval and use a special secondary antibody. For antigen retrieval, prepare a citrate buffer by mixing 19ml of 0.1M citric acid and 82ml of 0.1M sodium citrate to make 1000ml of buffer. Adjust the pH to 6.0 before each use. Add buffer to your slide. Using an 800 watt microwave oven with rotation, microwave for 7.5 min at high, 5 min at 50% (3 cycles), checking the slide at the end of each cycle to see if the solution needs to be replaced, adding water if needed. For the secondary antibody, use a biotinylated isotype specific secondary IGG (IGG-1) and a 3% donkey serum block. This will help reduce the background issues using mouse monoclonals.

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      Catalogue Number Description  
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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies