05-983 | Anti-PKC Antibody (α, β, γ), clone M110

05-983
100 µL  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RELISA, IP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-983
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PKC Antibody (α, β, γ), clone M110
      Alternate Names
      • PRKCA
      • PRKCB
      • PRKCG
      • protein kinase C, alpha, beta, gamma
      References
      Product Information
      FormatPurified
      Presentation100 μL of protein A purified mouse monoclonal IgG1 in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
      Applications
      ApplicationAnti-PKC (α Antibody, β, γ), clone M110 detects level of PKC (α & has been published & validated for use in ELISA, IP, WB & IC.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenRecombinant protein of human PKCγ amino acids 499-697.
      Cloneclone M110
      HostMouse
      SpecificityRecognizes PKC α, β, γ. Does not cross react with other PKC isoforms.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
      Gene Symbol
      • PRKCA
      • PKC-A
      • AAG6
      • PKC-alpha
      • MGC129901
      • MGC129900
      • PRKACA
      • PKCA
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P17252 # PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
      COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
      SIZE: 672 amino acids; 76764 Da
      SUBUNIT: Interacts with CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine.
      SIMILARITY: SwissProt: P17252 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
      Molecular Weight80-82 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PKC (#945;,#946;,#947;), Clone M110 (Protein A purified mouse monoclonal IgG1) - 2115576 2115576
      Anti-PKC (#945;,#946;,#947;), Clone M110 (Protein A purified mouse monoclonal IgG1) - 2013942 2013942
      Anti-PKC (#945;,#946;,#947;), Clone M110 - 2145962 2145962
      Anti-PKC (#945;,#946;,#947;), Clone M110 - NG1849979 NG1849979
      Anti-PKC (a,B,y), Clone M110 (Protein A purified mouse monoclonal IgG1) 2957951
      Anti-PKC (a,b,g), Clone M110 (Protein A purified mouse monoclonal IgG1) - 2205926 2205926
      Anti-PKC (a,b,g), Clone M110 (Protein A purified mouse monoclonal IgG1) - 2251761 2251761
      Anti-PKC (a,b,g), Clone M110 (Protein A purified mouse monoclonal IgG1) - 2262693 2262693
      Anti-PKC (a,b,g), Clone M110 (Protein A purified mouse monoclonal IgG1) - 2328026 2328026
      Anti-PKC (a,b,g), Clone M110 - 2055458 2055458

      References

      Reference overviewApplicationPub Med ID
      Evolution of the eye transcriptome under constant darkness in Sinocyclocheilus cavefish.
      Meng, F; Braasch, I; Phillips, JB; Lin, X; Titus, T; Zhang, C; Postlethwait, JH
      Molecular biology and evolution  30  1527-43  2013

      Show Abstract
      Immunohistochemistry23612715 23612715
      Protein kinase Cgamma regulates myosin IIB phosphorylation, cellular localization, and filament assembly
      Rosenberg, Michael and Ravid, Shoshana
      Mol Biol Cell, 17:1364-74 (2006)  2006

      16394101 16394101
      Protein kinase C modulates agonist-sensitive release of Ca<sup>2+</sup> from internal stores in HEK293 cells overexpressing the calcium sensing receptor
      Sakwe, Amos M, et al
      J Biol Chem, 280:4436-41 (2005)  2005

      15572354 15572354
      PKC-beta controls I kappa B kinase lipid raft recruitment and activation in response to BCR signaling.
      Su, Thomas T, et al.
      Nat. Immunol., 3: 780-6 (2002)  2002

      Show Abstract
      12118249 12118249