06-813 | Anti-PLK1 Antibody, NT

06-813
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-813
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-PLK1 Antibody, NT
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
      PresentationProtein A Purified immunoglobulin in 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl and 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-PLK1 Antibody, NT is an antibody against PLK1 for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide (KAGKLARAPADLGKGGVPG) corresponding to the amino acids 6 to 24 of mouse PLK1 (Polo-like Kinase 1)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes PLK1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PLK1
      • PLK
      • PLK-1
      • STPK13
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P53350 # May be required for cell division and may have a role during G1 or S phase.
      SIZE: 603 amino acids; 68255 Da
      SUBUNIT: Interacts with CEP170 and EVI5.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Placenta and colon.
      DEVELOPMENTAL STAGE: Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
      PTM: Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137. & Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.
      SIMILARITY: SwissProt: P53350 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. & Contains 2 POLO box domains. & Contains 1 protein kinase domain.
      Molecular Weight64 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on lysates from A431, HeLa, Jurkat, Raji, 3T3, CTLL, L6 or PC-12 cell lysates
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-PLK1, NT - 23454 23454
      Anti-PLK1, NT - 26589 26589
      Anti-PLK1, NT - 28004 28004
      Anti-PLK1, NT - 32659 32659
      Anti-PLK1, NT - DAM1828802 DAM1828802

      References

      Reference overviewApplicationPub Med ID
      Cdk1 phosphorylates the Rac activator Tiam1 to activate centrosomal Pak and promote mitotic spindle formation.
      Whalley, HJ; Porter, AP; Diamantopoulou, Z; White, GR; Castañeda-Saucedo, E; Malliri, A
      Nature communications  6  7437  2015

      Show Abstract
      26078008 26078008
      Cell cycle-dependent localization of CHK2 at centrosomes during mitosis.
      Chouinard, G; Clément, I; Lafontaine, J; Rodier, F; Schmitt, E
      Cell division  8  7  2013

      Show Abstract
      23680298 23680298
      Plk4 is required for cytokinesis and maintenance of chromosomal stability.
      Carla O Rosario,Michael A Ko,Yosr Z Haffani,Rebecca A Gladdy,Jana Paderova,Aaron Pollett,Jeremy A Squire,James W Dennis,Carol J Swallow
      Proceedings of the National Academy of Sciences of the United States of America  107  2010

      Show Abstract Full Text Article
      20348415 20348415
      Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells.
      Burkard, ME; Maciejowski, J; Rodriguez-Bravo, V; Repka, M; Lowery, DM; Clauser, KR; Zhang, C; Shokat, KM; Carr, SA; Yaffe, MB; Jallepalli, PV
      PLoS biology  7  e1000111  2009

      Show Abstract
      Western Blotting19468302 19468302
      Cell polarization during monopolar cytokinesis.
      Hu, CK; Coughlin, M; Field, CM; Mitchison, TJ
      The Journal of cell biology  181  195-202  2008

      Show Abstract Full Text Article
      18411311 18411311
      Nuclear cyclin B1 in human breast carcinoma as a potent prognostic factor.
      Takashi Suzuki,Tomohiro Urano,Yasuhiro Miki,Takuya Moriya,Jun-ichi Akahira,Takanori Ishida,Kuniko Horie,Satoshi Inoue,Hironobu Sasano
      Cancer science  98  2007

      Show Abstract
      17359284 17359284
      The ubiquitin-conjugating enzyme E2-EPF is overexpressed in primary breast cancer and modulates sensitivity to topoisomerase II inhibition.
      Tedesco, D; Zhang, J; Trinh, L; Lalehzadeh, G; Meisner, R; Yamaguchi, KD; Ruderman, DL; Dinter, H; Zajchowski, DA
      Neoplasia (New York, N.Y.)  9  601-13  2007

      Show Abstract Full Text Article
      17710163 17710163
      Mitogenic signalling and the p16INK4a-Rb pathway cooperate to enforce irreversible cellular senescence.
      Akiko Takahashi, Naoko Ohtani, Kimi Yamakoshi, Shin-ichi Iida, Hidetoshi Tahara, Keiko Nakayama, Keiichi I Nakayama, Toshinori Ide, Hideyuki Saya, Eiji Hara
      Nature cell biology  8  1291-7  2006

      Show Abstract
      17028578 17028578
      Clues to CD2-associated protein involvement in cytokinesis.
      Monzo, P; Gauthier, NC; Keslair, F; Loubat, A; Field, CM; Le Marchand-Brustel, Y; Cormont, M
      Molecular biology of the cell  16  2891-902  2005

      Show Abstract Full Text Article
      15800069 15800069
      Hierarchical requirement of SWI/SNF in retinoblastoma tumor suppressor-mediated repression of Plk1.
      Gunawardena, RW; Siddiqui, H; Solomon, DA; Mayhew, CN; Held, J; Angus, SP; Knudsen, ES
      The Journal of biological chemistry  279  29278-85  2004

      Show Abstract
      15105433 15105433

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies