07-1594 | Anti-Phospho-Dnmt1(Ser714) Antibody

07-1594
400 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISA, DBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1594
      DescriptionAnti-Phospho-Dnmt1(Ser714) Antibody
      Alternate Names
      • CXXC finger protein 9
      • CXXC-type zinc finger protein 9
      • DNA (cytosine-5-)-methyltransferase 1
      • DNA MTase HsaI
      • DNA methyltransferase 1
      • DNA methyltransferase HsaI
      Background InformationMethylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes may be linked with development of cancer. Three families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of Dnmt1 causes global demethylation of genomic DNA and embryonic lethality. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Phosphorylation of Ser714 is induced by EGF, but the role of this modification is unknown.
      References
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract or EGF-treated whole HeLa cell extract.
      PresentationPurified rabbit polyclonal in buffer containing 0.09% NaN3.
      Applications
      ApplicationAnti-Phospho-Dnmt1(Ser714) Antibody is a rabbit polyclonal antibody for detection of Phospho-Dnmt1(Ser714) also known as CXXC finger protein 9, DNA (cytosine-5-)-methyltransferase 1 & DNA MTase HsaI has been validated in WB, ELISA, DB.
      Key Applications
      • Western Blotting
      • ELISA
      • Dot Blot
      Application NotesELISA:
      This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).

      Dot blotting:
      This antibody has been shown by an outside laboratory to be specific for the phospho-Ser714 immunogen peptide, but not the unphosphorylated peptide, by dot blotting (1:500).
      Biological Information
      ImmunogenThe immunogen was a KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser714 of human Dnmt1.
      EpitopeSer714
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes human Dnmt1 phosphorylated on Ser714.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Not tested in other species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryDNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

      Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.
      Gene Symbol
      • AIM
      • CXXC9
      • DNMT
      • Dnmt1
      • EC 2.1.1.37
      • FLJ16293
      • M.HsaI
      • MCMT
      • MGC104992
      Modifications
      • Phosphorylation
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: Methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. Ref.17

      Catalytic activity S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

      SUBUNIT STRUCTURE: Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Component of complexes containing SUV39H1. Interacts with DNMT3A and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Ref.17 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13

      SUBCELLULAR LOCATION: Nucleus. Ref.13

      TISSUE SPECIFICITY: Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. Ref.12

      INDUCTION: Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle.

      SIMILARITY: Belongs to the C5-methyltransferase family.

      Contains 2 BAH domains.

      Contains 1 CXXC-type zinc finger.

      SEQUENCE CAUTION:The sequence AAD54507.1 differs from that shown. Reason: Erroneous gene model prediction.

      Molecular Weight~184 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      1:250 dilution of this antibody detected Dnmt1 on 10 µg HeLa nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size400 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti- Phospho-Dnmt1(Ser714) - NG1599501 NG1599501
      Anti-phospho-Dnmt1 (Ser714) - 2529403 2529403
      Anti-phospho-Dnmt1 (Ser714) -2630906 2630906
      Anti-phospho-Dnmt1 (Ser714) -2712441 2712441
      Anti-phospho-Dnmt1 (Ser714) Polyclonal Antibody 2952661

      References

      Reference overviewPub Med ID
      Essential role of p18Hamlet/SRCAP-mediated histone H2A.Z chromatin incorporation in muscle differentiation.
      Ana Cuadrado,Nadia Corrado,Eusebio Perdiguero,Vanesa Lafarga,Pura Muñoz-Canoves,Angel R Nebreda
      The EMBO journal  29  2010

      Show Abstract Full Text Article
      20473270 20473270

      Brochure

      Title
      Product Selection Guide - Antibodies, small molecule inhibitors, kits, assays and proteins for signaling research.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies