05-777-AF647 | Anti-Phosphotyrosine Antibody, recombinant clone 4G10® Antibody, Alexa Fluor® 647

100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AICCMAlexaFluor®647Monoclonal Antibody
      Catalogue Number05-777-AF647
      DescriptionAnti-Phosphotyrosine Antibody, recombinant clone 4G10® Antibody, Alexa Fluor® 647
      Background InformationPhosphorylation plays an important role in regulating protein activities and various cellular signaling events. Posttranslational phosphorylation can occur on histidine (pHis), serine (pSer), threonine (pThr), and tyrosine (pTyr) residues. Due to the early advent of anti-pTyr antibodies, tyrosine phosphorylation remains the mostly studied signaling events in all biological research fields. Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Before the availability of anti-pTyr antibodies, protein tyrosine phosphorylation was routiniely detected by time-consuming radioactive labeling. Although radioactive labeling is still considered as more sensitive, advancement in detection technology has greatly improved the detection limits of antibody-based methods. Anti-pTyr antibodies are commonly used in ELISA, immunoprecipitation, immunoblotting (including dot blot and Western blot), as well as indirect immunofluourescent detection of tyrosine phosphorylation in tissue and cell samples.
      Product Information
      PresentationPurified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      ApplicationAnti-Phosphotyrosine Antibody, clone 4G10, Alexa Fluor® 647 is a highly validated recombinant mouse monoclonal antibody that targets protein tyrosine phosphorylation and has been tested in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesThis recombinant monoclonal antibody is also available as HRP conjugate (Cat. No. 16-184), agarose conjugate (Cat. No. 16-199), biotin conjugate (Cat. No. 16-204), FITC conjugate (Cat. No. 16-205), PE conjugate (Cat. No. FCMAB323PE), as well as as unconjugated antibody (Cat. No. 05-777) for immunoaffinity purification, flow cytometry, immunocytochemistry, immunoprecipitation, and Western blotting applications.
      Biological Information
      ImmunogenProduced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Purified via heavy chain C-terminus hexa-histidine tag by Nickel affinity matrices prior to conjugation with Alexa Fluor® 647.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityRecognizes tyrosine-phosphorylated proteins from all species.
      Species Reactivity
      • All
      Species Reactivity NoteAll. Target modification is not species-specific.
      Antibody TypeMonoclonal Antibody
      • Phosphorylation
      Purification MethodProtein G purified.
      Molecular WeightVariable depending on the size(s) of the tyrosine-phosphorylated protein(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected EGF-induced tyrosine phosphorylation of cellular proteins in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information