05-988 | Anti-Pras40 Antibody, Clone 73P21

05-988
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-988
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Pras40 Antibody, Clone 73P21
      Alternate Names
      • 40 kDa proline-rich AKT substrate
      • AKT1 substrate 1 (proline-rich)
      • proline-rich Akt substrate, 40 kDa
      Background InformationPRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
      References
      Product Information
      FormatPurified
      Control
      • RIPA lysates of HeLa cells.
      PresentationPurified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.1% sodium azide.
      Applications
      ApplicationAnti-Pras40 Antibody, Clone 73P21 is an antibody against Pras40 for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoblot Analysis: A 01-1.0 μg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
      Biological Information
      ImmunogenRecombinant human PRAS40 (full-length) expressed in E. coli.
      Clone73P21
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes PRAS40.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. This antibody does not cross react with mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]
      Gene Symbol
      • AKT1S1
      • Lobe
      • MGC2865
      • PRAS40
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q96B36 # May play an important role in phosphatidylinositol 3- kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
      SIZE: 256 amino acids; 27383 Da
      SUBUNIT: The phosphorylated form interacts with 14-3-3.
      SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity). Note=Found in the cytosolic fraction of the brain (By similarity).
      TISSUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
      Molecular Weight40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot.

      Western Blot Analysis:
      A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 years at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-PRAS40 (Proline-Rich AKT Substrate) (Purified Mouse Monoclonal IgG1) - DAM1411312 DAM1411312
      Anti-PRAS40 (Proline-Rich AKT Substrate) (Purified Mouse Monoclonal IgG1) - DAM1462558 DAM1462558
      Anti-PRAS40 (Proline-Rich AKT Substrate) - DAM1415020 DAM1415020
      Anti-PRAS40 (Proline-Rich AKT Substrate) - DAM1512128 DAM1512128
      Anti-Pras40, Clone 73P21 - 2386932 2386932
      Anti-Pras40, Clone 73P21 - 2492052 2492052
      Anti-Pras40, Clone 73P21 -2567086 2567086
      Anti-Pras40, Clone 73P21 Monoclonal Antibody 3000949
      anti-Pras40 - 0604027267 0604027267
      anti-Pras40 - 0608039332 0608039332

      References

      Reference overviewPub Med ID
      Evaluating the therapeutic potential of mTOR inhibitors using mouse genetics.
      Huawei Li,Jennifer L Cotton,David A Guertin
      Methods in molecular biology (Clifton, N.J.)  821  2012

      Show Abstract
      22125076 22125076
      Rapid aminoacidemia enhances myofibrillar protein synthesis and anabolic intramuscular signaling responses after resistance exercise.
      West, DW; Burd, NA; Coffey, VG; Baker, SK; Burke, LM; Hawley, JA; Moore, DR; Stellingwerff, T; Phillips, SM
      The American journal of clinical nutrition  94  795-803  2011

      Show Abstract
      21795443 21795443