06-1102 | Anti-Protein patched homolog 1 Antibody

06-1102
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1102
      DescriptionAnti-Protein patched homolog 1 Antibody
      Alternate Names
      • PTCH protein +12b
      • PTCH protein +4
      • PTCH protein -10
      • patched (Drosophila) homolog
      • patched homolog (Drosophila)
      • patched homolog 1 (Drosophila)
      Background InformationProtein patched homolog 1 is a receptor for sonic hedgehog, a member of the hedgehog family of cell signaling proteins. Hedgehog signaling is regulated by Protein patched homolog 1 which keeps the pathway turned off in the absences of activation. This inhibits the function of associated downstream network components such as Smoothened (Smo).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human skeletal muscle, fetal tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Applications
      ApplicationAnti-Protein patched homolog 1 Antibody detects level of Protein patched homolog 1 & has been published & validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: 1:300 dilution from a previous lot detected Protein patched homolog 1 in kidney tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Protein patched homolog 1.
      EpitopeCytoplasmic domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the cytoplasmic domain of Protein patched homolog 1.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human and mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq].
      Gene Symbol
      • PTCH1
      • BCNS
      • HPE7
      • NBCCS
      • PTC
      • PTC1
      • PTCH
      • PTCH11
      • patched
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.

      SUBUNIT STRUCTURE: Interacts with SNX17.

      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

      TISSUE SPECIFICITY: In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.

      DEVELOPMENTAL STAGE: In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.

      PTM: Glycosylation is necessary for SHH binding By similarity.

      INVOLVEMENT IN DISEASE: Defects in PTCH1 are probably the cause of basal cell nevus syndrome (BCNS) [MIM:109400]; also known as Gorlin syndrome or Gorlin-Goltz syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas (NBCCS) and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas (BCC), fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 is also mutated in squamous cell carcinoma (SCC). Could also be associated with large body size observed in BCNS patients.

      Defects in PTCH1 are a cause of sporadic basal cell carcinoma (BCC) [MIM:605462].

      Defects in PTCH1 are the cause of holoprosencephaly type 7 (HPE7) [MIM:610828]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability.

      SEQUENCE SIMLARITIES: Belongs to the patched family.

      Contains 1 SSD (sterol-sensing) domain.
      Molecular Weight~ 160 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human skeletal muscle, fetal tissue lysate.

      Western Blot Analysis: 0.1 µg/ml of this antibody detected Protein patched homolog 1 on 10 µg of human skeletal muscle, fetal tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Protein patched homolog 1 - 2410988 2410988
      Anti-Protein patched homolog 1 - 1951957 1951957
      Anti-Protein patched homolog 1 - 2091766 2091766
      Anti-Protein patched homolog 1 - 2165861 2165861
      Anti-Protein patched homolog 1 - 2492786 2492786
      Anti-Protein patched homolog 1 - NG1857509 NG1857509
      Anti-Protein patched homolog 1 - NRG1702349 NRG1702349
      Anti-Protein patched homolog 1 -2712470 2712470
      Anti-Protein patched homolog 1 -2751171 2751171
      Anti-Protein patched homolog 1 Polyclonal Antibody 2926492