05-623 | Anti-RNA polymerase II Antibody, clone CTD4H8

200 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Yeast (S. cerevisiae)WB, ChIP-seqMPurifiedMonoclonal Antibody
      Catalogue Number05-623
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-RNA polymerase II Antibody, clone CTD4H8
      Background InformationRNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
      Product Information
      • A431 nuclear extract.
      PresentationPurified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
      ApplicationAnti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.
      Key Applications
      • Western Blotting
      • ChIP-seq
      Biological Information
      ImmunogenPeptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes Phospho- and non-phospho-RNA polymerase II.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Yeast (S. cerevisiae)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G Chromatography
      UniProt Number
      Molecular Weight210-220 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on HeLa nuclear extracts.

      Western Blot Analysis:
      0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RNA polymerase II, clone -2551215 2551215
      Anti-RNA polymerase II, clone -2605369 2605369
      Anti-RNA polymerase II, clone -2810725 2810725
      Anti-RNA polymerase II, clone CTD4H8 2476965
      Anti-RNA polymerase II, clone CTD4H8 (mouse monoclonal IgG1) - DAM1437261 DAM1437261
      Anti-RNA polymerase II, clone CTD4H8 (mouse monoclonal IgG1) - DAM1447120 DAM1447120
      Anti-RNA polymerase II, clone CTD4H8 - 2138015 2138015
      Anti-RNA polymerase II, clone CTD4H8 - 2378012 2378012
      Anti-RNA polymerase II, clone CTD4H8 - 2397109 2397109
      Anti-RNA polymerase II, clone CTD4H8 - 2452519 2452519


      Reference overviewApplicationSpeciesPub Med ID
      miR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program.
      Su, S; Zhao, Q; He, C; Huang, D; Liu, J; Chen, F; Chen, J; Liao, JY; Cui, X; Zeng, Y; Yao, H; Su, F; Liu, Q; Jiang, S; Song, E
      Nature communications  6  8523  2015

      Show Abstract
      26436920 26436920
      Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area.
      Koo, JW; Mazei-Robison, MS; LaPlant, Q; Egervari, G; Braunscheidel, KM; Adank, DN; Ferguson, D; Feng, J; Sun, H; Scobie, KN; Damez-Werno, DM; Ribeiro, E; Peña, CJ; Walker, D; Bagot, RC; Cahill, ME; Anderson, SA; Labonté, B; Hodes, GE; Browne, H; Chadwick, B; Robison, AJ; Vialou, VF; Dias, C; Lorsch, Z; Mouzon, E; Lobo, MK; Dietz, DM; Russo, SJ; Neve, RL; Hurd, YL; Nestler, EJ
      Nature neuroscience  18  415-22  2015

      Show Abstract
      25643298 25643298
      Insulin-response epigenetic activation of Egr-1 and JunB genes at the nuclear periphery by A-type lamin-associated pY19-Caveolin-2 in the inner nuclear membrane.
      Jeong, K; Kwon, H; Lee, J; Jang, D; Pak, Y
      Nucleic Acids Res  43  3114-27  2015

      Show Abstract
      25753664 25753664
      An anti-silencer- and SATB1-dependent chromatin hub regulates Rag1 and Rag2 gene expression during thymocyte development.
      Hao, B; Naik, AK; Watanabe, A; Tanaka, H; Chen, L; Richards, HW; Kondo, M; Taniuchi, I; Kohwi, Y; Kohwi-Shigematsu, T; Krangel, MS
      The Journal of experimental medicine  212  809-24  2015

      Show Abstract
      25847946 25847946
      Dgcr8 and Dicer are essential for sex chromosome integrity during meiosis in males.
      Modzelewski, AJ; Hilz, S; Crate, EA; Schweidenback, CT; Fogarty, EA; Grenier, JK; Freire, R; Cohen, PE; Grimson, A
      Journal of cell science  128  2314-27  2015

      Show Abstract
      25934699 25934699
      The Tat Inhibitor Didehydro-Cortistatin A Prevents HIV-1 Reactivation from Latency.
      Mousseau, G; Kessing, CF; Fromentin, R; Trautmann, L; Chomont, N; Valente, ST
      mBio  6  e00465  2015

      Show Abstract
      26152583 26152583
      Analysis of Histones H3 and H4 Reveals Novel and Conserved Post-Translational Modifications in Sugarcane.
      Moraes, I; Yuan, ZF; Liu, S; Souza, GM; Garcia, BA; Casas-Mollano, JA
      PloS one  10  e0134586  2015

      Show Abstract
      26226299 26226299
      Mammalian E-type cyclins control chromosome pairing, telomere stability and CDK2 localization in male meiosis.
      Martinerie, L; Manterola, M; Chung, SS; Panigrahi, SK; Weisbach, M; Vasileva, A; Geng, Y; Sicinski, P; Wolgemuth, DJ
      PLoS genetics  10  e1004165  2014

      Show Abstract
      24586195 24586195
      XBP1 promotes triple-negative breast cancer by controlling the HIF1α pathway.
      Chen, X; Iliopoulos, D; Zhang, Q; Tang, Q; Greenblatt, MB; Hatziapostolou, M; Lim, E; Tam, WL; Ni, M; Chen, Y; Mai, J; Shen, H; Hu, DZ; Adoro, S; Hu, B; Song, M; Tan, C; Landis, MD; Ferrari, M; Shin, SJ; Brown, M; Chang, JC; Liu, XS; Glimcher, LH
      Nature  508  103-7  2014

      Show Abstract
      24670641 24670641
      O-GlcNAcase Expression is Sensitive to Changes in O-GlcNAc Homeostasis.
      Zhang, Z; Tan, EP; VandenHull, NJ; Peterson, KR; Slawson, C
      Frontiers in endocrinology  5  206  2014

      Show Abstract
      25520704 25520704

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