06-1130 | Anti-RNF168 Antibody

06-1130
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1130
      DescriptionAnti-RNF168 Antibody
      Alternate Names
      • E3 ubiquitin-protein ligase RNF168
      • RING finger protein 168
      Background InformationRNF168 is a ubiquitin ligand that binds to ubiquinated histone H2A and localizes to sites of DNA damage. RNF168-dependent chromatin modifications cause 53BP1 and BRCA1 to accumulated at the site. A mutation in RNF168 has been associated with RIDDLE syndrome, an immunodeficiency and radiosensitivity disorder. RNF168 is responsible for securing repair factors at sites of DNA damage. RNF168 has two MIU (motif interacting with ubiquitin) and one UMI (UIM- and MIU-related UBD) ubiquitin binding domains (UBDs) which are essential for proper localization of RNF168.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Jurkat cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-RNF168 Antibody is a Rabbit Polyclonal Antibody for detection of RNF168 also known as E3 ubiquitin-protein ligase RNF168 & has been validated in WB.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot of this antibody was used to immunoprecipitate RNF168 from whole cell lysates (Zhao, H., et al. (2014) Cell Cycle. 13(11):1–11).
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to the C-terminus of human RNF168.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes RNF168 at the C-terminus.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSB repair (Stewart et al., 2009 [PubMed 19203578]).[supplied by OMIM].
      Gene Symbol
      • RNF168
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: E3 ubiquitin-protein ligase required for accumulation of repair proteins to sites of DNA damage. Acts with UBE2N/UBC13 to amplify the RNF8-dependent histone ubiquitination. Recruited to sites of DNA damage at double-strand breaks (DSBs) by binding to ubiquitinated histone H2A and ubiquitinates histone H2A and H2AX, leading to amplify the RNF8-dependent H2A ubiquitination and promoting the formation of 'Lys-63'-linked ubiquitin conjugates. This leads to concentrate ubiquitinated histones H2A and H2AX at DNA lesions to the threshold required for recruitment of TP53BP1 and BRCA1.

      PATHWAY: Protein modification; protein ubiquitination.

      SUBUNIT STRUCTURE: Interacts with UBE2N/UBC13.

      SUBCELLULAR LOCATION: Nucleus. Note: Localizes to sites of DNA damage.

      DOMAIN: The MIU motifs (motif interacting with ubiquitin) mediate the interaction with ubiquitin and the localization at sites of DNA damage.

      INVOLVEMENT IN DISEASE: Defects in RNF168 are the cause of Riddle syndrome (RIDDLES) [MIM:611943]. Riddle syndrome is characterized by increased radiosensitivity, immunodeficiency, mild motor control and learning difficulties, facial dysmorphism, and short stature. Defects are probably due to impaired localization of TP53BP1 and BRCA1 at DNA lesions.

      SEQUENCE SIMILARITIES: Belongs to the RNF168 family.

      Contains 1 RING-type zinc finger.

      SEQUENCE CAUTION: The sequence BAB70801.1 differs from that shown. Reason: Erroneous initiation.

      The sequence BAC04060.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~79 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected RNF168 on 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RNF168 2475899
      Anti-RNF168 - 1993787 1993787
      Anti-RNF168 - 2087826 2087826
      Anti-RNF168 - 2273981 2273981
      Anti-RNF168 - 2363613 2363613
      Anti-RNF168 - NRG1896997 NRG1896997
      Anti-RNF168 -2514270 2514270
      Anti-RNF168 -2619692 2619692
      Anti-RNF168 -2652001 2652001

      References

      Reference overviewApplicationPub Med ID
      Modulation of LSD1 phosphorylation by CK2/WIP1 regulates RNF168-dependent 53BP1 recruitment in response to DNA damage.
      Peng, B; Wang, J; Hu, Y; Zhao, H; Hou, W; Zhao, H; Wang, H; Liao, J; Xu, X
      Nucleic acids research  43  5936-47  2015

      Show Abstract
      25999347 25999347
      BCL10 regulates RNF8/RNF168-mediated ubiquitination in the DNA damage response.
      Zhao, H; Zhu, M; Dou, G; Zhao, H; Zhu, B; Li, J; Liao, J; Xu, X
      Cell cycle (Georgetown, Tex.)  13  1777-87  2014

      Show Abstract
      24732096 24732096
      An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells.
      Muñoz, MC; Yanez, DA; Stark, JM
      Nucleic acids research  42  7720-33  2014

      Show Abstract
      Western Blotting24829461 24829461
      RING finger nuclear factor RNF168 is important for defects in homologous recombination caused by loss of the breast cancer susceptibility factor BRCA1.
      Muñoz, MC; Laulier, C; Gunn, A; Cheng, A; Robbiani, DF; Nussenzweig, A; Stark, JM
      The Journal of biological chemistry  287  40618-28  2012

      Show Abstract
      Western Blotting23055523 23055523

      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies