06-1130-I | Anti-RNF168 Antibody

06-1130-I
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1130-I
      Replaces06-1130
      DescriptionAnti-RNF168 Antibody
      Alternate Names
      • E3 ubiquitin-protein ligase RNF168
      • hRNF168
      • RING finger protein 168
      Background InformationE3 ubiquitin-protein ligase RNF168 (UniProt Q8IYW5; also known as hRNF168, RING finger protein 168) is encoded by the RNF168 gene (Gene ID 165918) in human. Ubiquitylation/Ubiquitination is an essential early signal in the DNA damage repair process. Multiple E3 ligases, including RNF2 (RING2), RNF8 and RNF168, mediate the addition of K63-linked polyubiquitin chains to histone 2A (H2A) and H2AX, which in turn recruits DNA damage repair proteins, e.g. RAP80, BRCA1. In addition, SUMOylation and NEDDylation are also involved in the DNA damage response. RNF168 is an a dual specificity E3 ligase that mediates both the ubiquitination and NEDDylation of H2A and H2AX. The two types of modifications compete against each other and NEDD8 modification of H2A and H2AX blocks the recruitment of BRCA1 at DNA damage repair sites. RNF168 itself is also subjected to NEDD8 modification and NEDDylation of RNF168 is necessary for its ubiquitin ligase activity. Inhibition of RNF168 NEDDylation impairs its interaction with E2 enzyme Ubc13 (UBE2N). Likewise, downregulating RNF168 NEDDylation by the deNEDDylating enzyme NEDP1, or due to mutations of the NEDD8-conjugating enzyme UBC12, decreases H2A and H2AX ubiquitylation. RNF168 Mutations are linked to RIDDLE (radiosensitivity, immunodeficiency dysmorphic features and learning difficulties) syndrome.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-RNF168, Cat. No. 06-1130-I, is a highly specific rabbit polyclonal antibody that targets RNF168 and has been tested in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a representative lot detected nuclear RNF168 immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized human B-cell hybridoma SA13.

      Immunoprecipitation Analysis: 20 µL from a representative lot immunoprecipitated RNF168 from 500 µg of human B-cell hybridoma SA13 cell lysate.
      Biological Information
      ImmunogenGST-tagged full-length human RNF168.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detected nuclear RNF168 immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized human B-cell hybridoma SA13.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNF168
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~75 kDa observed. 65.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SA13 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RNF168 in 10 µg of human B-cell hybridoma SA13 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications