09-271 | Anti-Rac1b Antibody

100 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IH(P), IFRbAffinity PurifiedPolyclonal Antibody
      Catalogue Number09-271
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Rac1b Antibody
      Alternate Names
      • Ras-related C3 botulinum toxin substrate 1
      • Cell migration-inducing gene 5 protein
      • Ras-like protein TC25
      • p21-Rac1
      Background InformationRas-related C3 botulinum toxin substrate 1 (UniProt: P63000; also known as Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1) is encoded by the RAC1 (also known as TC25, MIG5) gene (Gene ID: 5879) in human. Rac1 is a plasma membrane-associated small GTPase that cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses. Two isoforms of Rac1 have been described that are produced by alternative splicing. Rac1b differs from Rac1a in that it has an additional 19 amino acids and is found in various tumors, such as breasts and colorectal. Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It can bind to the GTPase-binding domain of PAK, but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 30% glycerol.
      ApplicationAnti-Rac1b Antibody, Cat. No. 09-271, is a rabbit polyclonal antibody that detects Ras-related C3 botulinum toxin substrate 1 (Rac1b) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Rac1b in HeLa, A431, HUVEC, and NIH/3T3 cells.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected Rac1b in human colon cancer and human prostate cancer tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Rac1b.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis rabbit polyclonal antibody specifically detects Rac1b in human and murine cells.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]
      Transcript Variant: This variant (Rac1b) includes the alternatively spliced 57 bp region (exon 3b) that is missing in transcript variant Rac1.
      Gene Symbol
      • RAC1
      • MGC111543
      • ORFNames=MIG5
      • PAK1
      • MIG5
      • TC-25
      • p21-Rac1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction.
      SIZE: 192 amino acids; 21450 Da
      SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2.
      SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
      DOMAIN: SwissProt: P63000 The effector region mediates interaction with DEF6.
      SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
      Molecular Weight~17 kDa observed; 23.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF7 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rac1b in MCF7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Rac1b 3082473
      Anti-Rac1b - 2111394 2111394
      Anti-Rac1b - 2187396 2187396
      Anti-Rac1b - 2298431 2298431
      Anti-Rac1b - DAM1503829 DAM1503829
      Anti-Rac1b - DAM1588289 DAM1588289
      Anti-Rac1b - NG1638566 NG1638566
      Anti-Rac1b - NG1658346 NG1658346
      Anti-Rac1b - NG1743550 NG1743550
      Anti-Rac1b - NG17524496 NG17524496


      Reference overviewPub Med ID
      Epithelial splicing regulatory proteins 1 (ESRP1) and 2 (ESRP2) suppress cancer cell motility via different mechanisms.
      Ishii, H; Saitoh, M; Sakamoto, K; Kondo, T; Katoh, R; Tanaka, S; Motizuki, M; Masuyama, K; Miyazawa, K
      The Journal of biological chemistry  289  27386-99  2014

      Show Abstract
      25143390 25143390
      Rac1b negatively regulates TGF-β1-induced cell motility in pancreatic ductal epithelial cells by suppressing Smad signalling.
      Ungefroren, H; Sebens, S; Giehl, K; Helm, O; Groth, S; Fändrich, F; Röcken, C; Sipos, B; Lehnert, H; Gieseler, F
      Oncotarget  5  277-90  2014

      Show Abstract
      24378395 24378395
      Ibuprofen inhibits colitis-induced overexpression of tumor-related Rac1b.
      Matos, P; Kotelevets, L; Goncalves, V; Henriques, AF; Henriques, A; Zerbib, P; Moyer, MP; Chastre, E; Jordan, P
      Neoplasia (New York, N.Y.)  15  102-11  2013

      Show Abstract
      23359345 23359345
      Involvement of hnRNP A1 in the matrix metalloprotease-3-dependent regulation of Rac1 pre-mRNA splicing.
      Pelisch, F; Khauv, D; Risso, G; Stallings-Mann, M; Blaustein, M; Quadrana, L; Radisky, DC; Srebrow, A
      Journal of cellular biochemistry  113  2319-29  2012

      Show Abstract
      22345078 22345078
      Rac1b regulates NT3-stimulated Mek-Erk signaling, directing marrow-isolated adult multilineage inducible (MIAMI) cells toward an early neuronal phenotype.
      Kevin M Curtis,Lourdes A Gomez,Paul C Schiller
      Molecular and cellular neurosciences  49  2012

      Show Abstract
      22061968 22061968
      Matrix metalloproteinase induction of Rac1b, a key effector of lung cancer progression.
      Stallings-Mann, ML; Waldmann, J; Zhang, Y; Miller, E; Gauthier, ML; Visscher, DW; Downey, GP; Radisky, ES; Fields, AP; Radisky, DC
      Science translational medicine  4  142ra95  2012

      Show Abstract
      22786680 22786680

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      Life Science Research > Antibodies and Assays > Primary Antibodies