05-525 | Anti-Rad50 Antibody, clone 13B3/2C6

05-525
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-525
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Rad50 Antibody, clone 13B3/2C6
      References
      Product Information
      FormatPurified
      Presentation10mM PBS, pH 7.4
      Applications
      ApplicationAnti-Rad50 Antibody, clone 13B3/2C6 is a high quality Mouse Monoclonal Antibody for the detection of Rad50 & has been validated in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 1-425 of human Rad50
      Clone13B3/2C6
      HostMouse
      SpecificityRad50
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
      Gene Symbol
      • RAD50
      • RAD50-2
      • hRad50
      • hRAD50
      • RAD50-PEN
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q92878 # Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
      COFACTOR: Binds 1 zinc ion per homodimer (By similarity).
      SIZE: 1312 amino acids; 153892 Da
      SUBUNIT: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis.
      SUBCELLULAR LOCATION: Nucleus. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents.TISSUE SPECIFICITY: Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
      DOMAIN: SwissProt: Q92878 The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc- hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer (By similarity).
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity).
      SIMILARITY: Belongs to the SMC family. RAD50 subfamily. & Contains 1 zinc-hook domain.
      MISCELLANEOUS: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.
      Molecular Weight153kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa nuclear lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Rad50, clone 13B3/2C6 2883130
      Anti-Rad50, clone 13B3/2C6 - 2424648 2424648
      Anti-Rad50, clone 13B3/2C6 - 1955409 1955409
      Anti-Rad50, clone 13B3/2C6 - 1958296 1958296
      Anti-Rad50, clone 13B3/2C6 - 2043183 2043183
      Anti-Rad50, clone 13B3/2C6 - 2204863 2204863
      Anti-Rad50, clone 13B3/2C6 - 2326276 2326276
      Anti-Rad50, clone 13B3/2C6 - 2519318 2519318
      Anti-Rad50, clone 13B3/2C6 - DAM1641085 DAM1641085
      Anti-Rad50, clone 13B3/2C6 - DAM1778029 DAM1778029

      References

      Reference overviewApplicationPub Med ID
      Hyperthermia alters the interaction of proteins of the Mre11 complex in irradiated cells.
      Gerashchenko, BI; Gooding, G; Dynlacht, JR
      Cytometry. Part A : the journal of the International Society for Analytical Cytology  77  940-52  2010

      Show Abstract
      Immunofluorescence21290468 21290468
      A tool for enhancement and scoring of DNA repair foci.
      Gerashchenko, BI; Dynlacht, JR
      Cytometry. Part A : the journal of the International Society for Analytical Cytology  75  245-52  2009

      Show Abstract
      Immunofluorescence18836995 18836995
      Functional role for senataxin, defective in ataxia oculomotor apraxia type 2, in transcriptional regulation.
      Suraweera, A; Lim, Y; Woods, R; Birrell, GW; Nasim, T; Becherel, OJ; Lavin, MF
      Human molecular genetics  18  3384-96  2009

      Show Abstract
      Western Blotting19515850 19515850
      Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95.
      Trujillo, K M, et al.
      J. Biol. Chem., 273: 21447-50 (1998)  1998

      Show Abstract
      9705271 9705271
      Mutations in the MRE11, RAD50, XRS2, and MRE2 genes alter chromatin configuration at meiotic DNA double-stranded break sites in premeiotic and meiotic cells.
      Ohta, K, et al.
      Proc. Natl. Acad. Sci. U.S.A., 95: 646-51 (1998)  1998

      Show Abstract
      9435246 9435246
      Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair.
      Dolganov, G M, et al.
      Mol. Cell. Biol., 16: 4832-41 (1996)  1996

      Show Abstract
      8756642 8756642

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies