05-516-AF555 | Anti-Ras Antibody, clone RAS10, Alexa Fluor® 555 Conjugate

05-516-AF555
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, RICC, FCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue Number05-516-AF555
      DescriptionAnti-Ras Antibody, clone RAS10, Alexa Fluor® 555 Conjugate
      Alternate Names
      • GTPase HRas
      • c-H-ras
      • H-Ras-1
      • Ha-Ras
      • p21ras
      • Transforming protein p21
      • GTPase KRas
      • c-K-ras
      • c-Ki-ras
      • K-Ras 2
      • Ki-Ras
      • GTPase NRas
      • Transforming protein N-Ras
      Background InformationRas proteins are small GTPases controlling cellular pathways that signal for growth, proliferation, and differentiation. Mammalian genomes harbor three Ras genes (HRAS, NRAS, and KRAS) that encode four GTPases, two of which (K-Ras4A and K-Ras4B) are transcribed from the KRAS gene via two alternative fourth exons. Ras proteins share nearly identical sequence homology in sequence encoded by exons 1-3 (a.a. 1-165), while diverge in their C-terminal 24-a.a. hypervariable region (HVR) encoded by exon 4. The first 165 amino acids form the catalytic G domain that mediates GDP/GTP binding and association with effectors, exchange factors, and GTPase-activating proteins (GAPs), while the C-terminal HVR is needed for Ras membrane localization, a process necessary for Ras function. In addition to the removal of initiator methionine (Met1), Ras proteins are posttranslationally modified by farnesylation of Cys186, followed by proteolytical cleavage their C-terminal end three amino acids (a.a. 187-189) and carboxyl methylation of the newly formed C-terminal prenylcysteine. H-Ras, N-Ras, and K-Ras4A are further palmitoylated on cysteine residue(s) near the C-terminus (Cys181/Cys184 in H-Ras, Cys181 in N- and K-Ras), while K-Ras4B lacks a palmitoylation site. Palmitoylation is required for targeting H- and N-Ras to the plasma membrane (PM), while K-Ras4A and K-Ras4B contain a series of lysines that constitute a polybasic region (PBR) capable of interacting with the inner leaflet of the PM through an electrostatic interaction.
      References
      Product Information
      FormatAlexaFluor®555
      PresentationPurified mouse IgG2a Alexa Fluor® 555 conjugate in buffer containing PBS with 1.5% BSA and 0.05% sodium azide.
      Applications
      ApplicationDetect Ras using this mouse monoclonal Anti-Ras Antibody, clone RAS10, Alexa Fluor® 555 Conjugate, Cat. No. 05-516-AF555, validated for use in Flow Cytometry aned Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 0.2 µL from a representative lot detected Ras immunoreactivity in one million A431 cells.

      Clone Ras10 is also available as unconjugated, as well as in FITC- and Alexa Fluor® 647-conjugated forms (Cat. Nos. 05-516, FCMAB148F, 05-516-AF647) for ELISA, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.
      Biological Information
      ImmunogenRecombinant human H-Ras p21 (Hamer, P.J., et al. (1990). Hybridoma.9(6):573-587).
      CloneRAS10
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityRas 10 reacts with normal p21, activated p21, and p21 from each of the Harvey, Kirsten and N-ras families. Ras 10 reacts with recombinant p21 as well as p21 derived from human and rodent cells (Hamer, P.J., et al. (1990). Hybridoma.9(6):573-587).
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HRAS
      • HRAS1
      • KRAS
      • CFC2
      • NS3
      • KRAS2
      • RASK2
      • NRAS
      • NS6
      • CMNS
      • NCMS
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~21 kDa observed. 21.30 kDa (human/mouse/rat H-Ras4A, p21 pro-form), 20.87 kDa (human/mouse/rat mature H-Ras4A, p21), 21.66 kDa (human/mouse/rat K-Ras4A pro-form), 21.17 kDa (human/mouse/rat mature K-Ras4A), 21.23/21.20/21.24 kDa (human/mouse/rat N-Ras pro-form), 20.90/20.90/20.91 kDa (human/mouse/rat mature N-Ras) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ras immunoreactivity in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications