04-822 | Anti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal

04-822
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, HWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-822
      Replaces05-822
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Rho (-A, -B, -C) Antibody, clone 3L74, rabbit monoclonal
      Alternate Names
      • ras homolog
      Background InformationRho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganisation, and membrane trafficking.
      References
      Product Information
      FormatCulture Supernatant
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      ApplicationThis Anti-Rho (-A Antibody, -B, -C), clone 3L74 is validated for use in WB for the detection of Rho (-A, -B, -C).
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis:
      A 1:1000-1:4000 dilution of this lot detected Rho (-A, -B, -C) in RIPA lysates from human HL-60 and rat PC-12 cells. This antibody also recognized recombinant RhoA protein, but not recombinant rac1 or cdc42 proteins.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (SYPDTDVILMCFSIDSPDSLENKK) corresponding to amino acids 73-94 of human Rho (-A, -B, -C), with two terminal lysines added to increase peptide solubility
      Clone3L74
      HostRabbit
      SpecificityRecognizes Rho (-A, -B, -C)
      IsotypeIgG
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteBroad species cross-reactivity expected based on sequence homology
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ARH12
      • ARHA
      • H12
      • RHOH12
      • RhoA
      • Rho12
      • RHO12
      • RHOA
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P61586 # Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1.
      COFACTOR: Magnesium.
      SIZE: 193 amino acids; 21768 Da
      SUBUNIT: Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13.
      SUBCELLULAR LOCATION: Membrane; Lipid-anchor.
      DOMAIN: SwissProt: P61586 The basic-rich region is essential for yopT recognition and cleavage.
      PTM: Substrate for botulinum ADP-ribosyltransferase. & Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.
      SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
      Molecular WeightMr 22kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on modified RIPA lysates from HL-60 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Rho (-A, -B, -C), clone 3L74 2482243
      Anti-Rho (-A, -B, -C), clone 3L74 - 2534854 2534854
      Anti-Rho (-A, -B, -C), clone 3L74 - DAM1561703 DAM1561703

      References

      Reference overviewPub Med ID
      Prognostic value of rho GTPases and rho guanine nucleotide dissociation inhibitors in human breast cancers.
      Jiang, Wen G, et al.
      Clin. Cancer Res., 9: 6432-40 (2003)  2003

      Show Abstract
      14695145 14695145
      The PRK2 kinase is a potential effector target of both Rho and Rac GTPases and regulates actin cytoskeletal organization.
      Vincent, S and Settleman, J
      Mol. Cell. Biol., 17: 2247-56 (1997)  1997

      Show Abstract
      9121475 9121475
      Signal-transducing protein phosphorylation cascades mediated by Ras/Rho proteins in the mammalian cell: the potential for multiplex signalling.
      Denhardt, D T
      Biochem. J., 318 ( Pt 3): 729-47 (1996)  1996

      Show Abstract
      8836113 8836113

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies