04-486 | Anti-RhoG Antibody, clone 1F3 B3 E5

04-486
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-486
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-RhoG Antibody, clone 1F3 B3 E5
      Alternate Names
      • ARHG
      • RHOG ras homolog gene family, member G (rho G)
      • MGC125835
      • MGC125836
      Background InformationRhoG (ARGH) is a member of the Ras super-family of small G-proteins and a member of the Rac subfamily of Rho Family of GTPases. RhoG is a small G-protein (GTPase) who is activated by a GEF (guanine nucleotide exchange factor). The GEF displaces GDP from the binding site of RhoG and allows GTP to bind in it place rendering the protein active. This active RhoG binds downstream effector molecules that include ELMO and DOCK180. RhoG then cleaves GTP to GDP making it inactive. RhoG is believed to be crucial for integrin-mediated Rac activation and cell spreading. RhoG is believed to be active upstream of Rac and work independently of Rac.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2b in storage buffer containing PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-RhoG Antibody, clone 1F3 B3 E5 is an antibody against RhoG for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH synthetic peptide encompassing amino acids CQQDGVKEVFAEAVRAVLNPT (RhoG 160-180)
      Cloneclone 1F3 B3 E5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes RhoG
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryARHG is a member of the RAS superfamily of genes, which encode GTP-binding proteins that act in the pathway of signal transduction and play a key role in the regulation of cellular functions.[supplied by OMIM]
      Gene Symbol
      • RHOG ras homolog gene family, member G (rho G)
      • Rho G
      • ARHG
      • MGC125835
      • MGC125836
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Required for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.
      SUBUNIT STRUCTURE:Interacts with SGEF.
      Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic sidePotential.
      Sequence similarities: Belongs to the small GTPase superfamily. Rho family.
      Molecular Weight21 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at 2-8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RhoG, clone 1F3 B3 E5 - 2392290 2392290
      Anti-RhoG, clone 1F3 B3 E5 - 2029684 2029684
      Anti-RhoG, clone 1F3 B3 E5 - 2087845 2087845
      Anti-RhoG, clone 1F3 B3 E5 - 2243494 2243494
      Anti-RhoG, clone 1F3 B3 E5 - DAM1597408 DAM1597408
      Anti-RhoG, clone 1F3 B3 E5 - DAM1690717 DAM1690717
      Anti-RhoG, clone 1F3 B3 E5 - DAM1739310 DAM1739310
      Anti-RhoG, clone 1F3 B3 E5 - DAM1785877 DAM1785877
      Anti-RhoG, clone 1F3 B3 E5 - DAM1824781 DAM1824781
      Anti-RhoG, clone 1F3 B3 E5 - NG1517764 NG1517764

      References

      Reference overviewPub Med ID
      A Trio-Rac1-Pak1 signalling axis drives invadopodia disassembly.
      Moshfegh, Y; Bravo-Cordero, JJ; Miskolci, V; Condeelis, J; Hodgson, L
      Nature cell biology  16  574-86  2014

      Show Abstract
      24859002 24859002
      miR-124-regulated RhoG reduces neuronal process complexity via ELMO/Dock180/Rac1 and Cdc42 signalling.
      Franke, K; Otto, W; Johannes, S; Baumgart, J; Nitsch, R; Schumacher, S
      The EMBO journal  31  2908-21  2012

      Show Abstract
      22588079 22588079