07-1477 | Anti-Rsk1 Antibody

07-1477
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IP, ELISARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1477
      Replaces04-417
      DescriptionAnti-Rsk1 Antibody
      Alternate Names
      • 90 kDa ribosomal protein S6 kinase 1
      • MAP kinase-activated protein kinase 1
      • Ribosomal S6 kinase 1
      • Ribosomal protein S6 kinase alpha-1
      • S6K-alpha 1
      • p90-RSK 1
      • Ribosomal protein S6 kinase alpha 1
      • Ribosomal protein S6 kinase, 90kD, 1
      • Ribosmal protein S6 kinase, 90kDa, polypeptide 1
      Background InformationRsk1, Rsk2, and Rsk3 (ribosomal protein S6 kinase, 90kDa, polypeptide 1,2,3; S6K-alpha 1,2,3) are Ser/Thr kinases which are activated by MAP Kinases, and are alternatively referred to as MAPKAP kinases 1a, 1b, and 1c. Following activation, Rsk isoforms translocate to the nucleus, where Rsk2 phosphorylates Ser10 of Histone H3, a critical step in the stimulation of immediate-early gene expression. Rsk1 is able to phosphorylate Ser133 of CREB, activating it as a transcription factor. Rsk1 activation can be monitored by phosphorylation state specific antibodies directed against sites involved in its activation.
      References
      Product Information
      FormatAffinity Purified
      Control
      • NIH/3T3 cell lysate
      PresentationPurified rabbit polyclonal IgG in buffer containing TBS with 0.01% Sodium Azide in 50% glycerol.
      Applications
      ApplicationAnti-Rsk1 Antibody is an antibody against Rsk1 for use in WB, IP, ELISA.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • ELISA
      Application NotesImmunoprecipitation: 4 µg of a previous lot of this antibody was used per 1500 µg of crude protein sample in Immunoprecipitation.

      ELISA: Recommended.
      Optimal working dilutions must be determined by end user.

      Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the c-terminal sequence of human p90 RSK1 (C-LAQRRVRKLPSTTL).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes p90 RSK1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
      Gene Symbol
      • RPS6KA1
      • HU-1
      • MAPKAPK1A
      • RSK
      • pp90RSK1
      • RSK-1
      • p90S6K
      • RSK1
      • EC 2.7.11.1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      COFACTOR: Magnesium.
      ENZYME REGULATION: Activated by multiple phosphorylations on threonine and serine residues.
      SUBUNIT: Forms a complex with either ERK1 or ERK2 in quiescent cells. Transiently dissociates following mitogenic stimulation.
      PTM: Autophosphorylated on Ser-380, as part of the activation process.
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
      SIMILARITY: Contains 1 AGC-kinase C-terminal domain.
      SIMILARITY: Contains 2 protein kinase domains.
      Molecular Weight83 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on NIH/3T3 lysates.

      Western Blot Analysis:
      1:500-1:1,000 dilution of this lot detected RSK1 on 10 μg of NIH/3T3 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-RSK1 2477817
      Anti-RSK1 - 2431958 2431958
      Anti-RSK1 - NG1554802 NG1554802
      Anti-RSK1 - NG1871101 NG1871101
      Anti-RSK1 -2673810 2673810
      Anti-RSK1 -2733913 2733913
      Anti-RSK1_2849063 2849063

      References

      Reference overviewPub Med ID
      Pricing of drugs and donations: options for sustainable equity pricing.
      C Pérez-Casas,E Herranz,N Ford
      Tropical medicine & international health : TM & IH  6  2001

      Show Abstract
      11703854 11703854

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies