05-733 | Anti-SETA/CIN85/Ruk/SH3KBPI Antibody, clone 84

05-733
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-733
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-SETA/CIN85/Ruk/SH3KBPI Antibody, clone 84
      References
      Product Information
      FormatPurified
      PresentationPrtotein G purified antibody in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4
      Applications
      ApplicationDetect SETA/CIN85/Ruk/SH3KBPI using this Anti-SETA/CIN85/Ruk/SH3KBPI Antibody, clone 84 validated for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      Immunogen6His and HA tagged fusion protein corresponding to full length rat SETA
      Cloneclone 84
      HostMouse
      SpecificitySETA
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCBL (MIM 165360) constitutively interacts with SH3 domain-containing proteins and, upon tyrosine phosphorylation, with SH2 domain-containing proteins. The SH3KBP1 gene encodes an 85-kD CBL-interacting protein that enhances tumor necrosis factor (MIM 191160)-mediated apoptotic cell death (Narita et al., 2001 [PubMed 11474197]).[supplied by OMIM]
      Gene Symbol
      • SH3KBP1
      • CD2BP3
      • OTTHUMP00000061480
      • HSB-1
      • GIG10
      • CIN85
      • MIG18
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q96B97 # Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis.
      SIZE: 665 amino acids; 73126 Da
      SUBUNIT: Can self-associate and form homotetramers. Interacts with CD2, F-actin capping protein, PIK3R3, GRB2, EGFR, MET, BLNK, MAP3K4, PDCD6IP, SPRY2, ARHGAP17, MAGI2, CRK, BCAR1, SOS1, DDEF1, CENTD3, HIP1R, SYNJ2, INPP5D and STAP1. Interacts with CBL and CBLB, but does not interact with CBLC. Two molecules of SH3KBP1 seem to bind through their respective SH3 1 domain to one molecule of CBLB. The interaction with CBL or CBLB and EGFR is increased upon EGF stimulation. The interaction with CBL is attenuated by PDCD6IP. Interacts through its proline-rich region with the SH3 domain of endophilins SH3GL1, SH3GL2 and SH3GL3. The SH3KBP1- endophilin complex seems to associate with a complex containing the phosphorylated receptor (EGFR or MET) and phosphorylated CBL. Probably associates with DDEF1 and phosphorylated EGFR. Probably part of a complex consisting of at least SH3KBP1, DDEF1 and CENTD3. Interacts with focal adhesion kinases PTK2 AND PTK2B, probably as a dimer. Interacts with DAB2 and probably associates with chathrin through its interaction with DAB2. Part of a complex consisting of SH3KBP1, DAB2, and clathrin heavy chain. DAB2 and clathrin dissociate from SH3KBP1 following growth factor treatment, enabling interaction with CBL. Interacts with DDN and probably associates with MAGI2 through its interaction with DDN. Interacts with the SH3 domains of SRC tyrosine-protein kinases SRC, LCK, LYN, FGR, FYN and HCK. Interacts with TRADD, BIRC2, TRAF1, TRAF2 and TNFR1, and the association with a TNFR1- associated complex upon stimulation with TNF-alpha seems to be mediated by SRC. Probably interacts with SH3KBP1.
      SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome; Peripheral membrane protein. Note=Localized in endocytic vesicles containing clustered receptors. Colocalizes with DDEF1 in vesicular structures. Colocalized with actin microfilaments and focal adhesions (By similarity). Colocalized with MAGI2 in synaptosomes (By similarity).TISSUE SPECIFICITY: Ubiquitously expressed. Also expressed in some cancer cell lines.DOMAIN:SwissProt: Q96B97 The SH3 domains mediate interaction with SHKBP1 (By similarity).
      PTM: Monoubiquitinated by CBL and CBLB after EGF stimulation; probably on its C-terminus.
      SIMILARITY: Contains 3 SH3 domains.
      Molecular Weight85kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from NG108-15 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SETA/CIN85/Ruk/SH3KBPI, clone 84 - 24653 24653
      Anti-SETA/CIN85/Ruk/SH3KBPI, clone 84 -2612796 2612796

      References

      Reference overviewApplicationPub Med ID
      CIN85 associates with endosomal membrane and binds phosphatidic acid.
      Jing Zhang, Xiudan Zheng, Xiao Yang, Kan Liao, Jing Zhang, Xiudan Zheng, Xiao Yang, Kan Liao
      Cell research  19  733-46  2009

      Show Abstract
      19417776 19417776
      Studying protein isoforms of the adaptor SETA/CIN85/Ruk with monoclonal antibodies.
      Finniss, Susan, et al.
      Biochem. Biophys. Res. Commun., 325: 174-82 (2004)  2004

      Show Abstract
      Immunoblotting (Western)15522216 15522216
      SETA: a novel SH3 domain-containing adapter molecule associated with malignancy in astrocytes.
      Bögler, O, et al.
      Neuro-oncology, 2: 6-15 (2000)  2000

      11302255 11302255
      The glioma-associated protein SETA interacts with AIP1/Alix and ALG-2 and modulates apoptosis in astrocytes.
      Chen, B, et al.
      J. Biol. Chem., 275: 19275-81 (2000)  2000

      Show Abstract
      10858458 10858458

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies