06-1363 | Anti-SRp30a (SF2) Antibody

06-1363
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-1363
      DescriptionAnti-SRp30a (SF2) Antibody
      Alternate Names
      • serine/arginine-rich splicing factor 1
      • pre-mRNA-splicing factor SF2, P33 subunit
      • SR splicing factor 1
      • splicing factor 2
      • splicing factor, arginine/serine-rich 1
      • Alternative-splicing factor 1
      Background InformationSRp30a, also known as SF2, ASF-1 (alternative splicing factor 1), is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.
      References
      Product Information
      FormatPurified
      Control
      • NIH/3T3 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationUse Anti-SRp30a (SF2) Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect SRp30a (SF2) also known as serine/arginine-rich splicing factor 1, pre-mRNA-splicing factor SF2- P33 subunit, SR splicing factor 1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis:
      1:500 dilution from a representative lot detected SRp30a in NIH/3T3 cells.
      Biological Information
      ImmunogenLinear peptide corresponding to human SRp30a.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 13. [provided by RefSeq].
      Gene Symbol
      • ASF-1
      • SF2
      • ASF
      • SFRS1
      • SF2p33
      • SRp30a
      • SRSF1
      • SF2P333
      Purification MethodProtein A
      UniProt Number
      UniProt SummaryFUNCTION: Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.

      SUBUNIT STRUCTURE: Consists of two polypeptides of p32 and p33. In vitro, self-associates and binds SRSF2, SNRNP70 and U2AF1 but not U2AF2. Binds SFRS12. Interacts with SAFB/SAFB1. Interacts with PSIP1/LEDGF. Interacts with SRPK1. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SRSF1/SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Interacts with RSRC1 (via Arg/Ser-rich domain). Interacts with ZRSR2/U2AF1-RS2.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus speckle. Note: In nuclear speckles. Shuttles between the nucleus and the cytoplasm.

      DOMAIN: Both the RS domain and an RRM domain are required for nucleocytoplasmic shuttling.

      PTM: Extensively phosphorylated on serine residues in the RS domain. This may regulate nucleocytoplasmic shuttling.

      Arg-97 is dimethylated, probably to asymmetric dimethylarginine.

      SEQUENCE SIMILARITIES: Belongs to the splicing factor SR family.

      Contains 2 RRM (RNA recognition motif) domains.
      Molecular Weight~40 kDa observed. An uncharacterized band at ~ 50 kDa has been observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in NIH/3T3 cell lysate.

      Western Blot Analysis: 1:500 dilution of this antibody detected SRp30a on 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies