07-550 | Anti-SUV39H1 Antibody

07-550
200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-550
      ReplacesMABE552
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-SUV39H1 Antibody
      Alternate Names
      • KMT1A
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
      Applications
      ApplicationUse Anti-SUV39H1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect SUV39H1 also known as KMT1A, Histone H3-K9 Methyltransferase 1.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPeptide (C-QVDPVDMESTRMDSNFGLAGLPGSP) corresponding to amino acids 368-392 of mouse suppressor of variegation 3-9 homolog 1 (SUV39H1).
      HostRabbit
      SpecificitySUV39H1
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene SummaryThis gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase, methylating Lys-9 of histone H3. Overall, it plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression.
      Gene Symbol
      • SUV39H1
      • SUV39H
      • Su
      • MG44
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43463 # Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys- 9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiaton, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length.


      SIZE: 412 amino acids; 47907 Da

      SUBUNIT: Interacts with H3 and H4 histones. Interacts with GFI1B, DNMT3B, CBX1, CBX4, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1. Interacts with SBF1 through the SET domain. Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. In case of infection, interacts with HTLV-1 Tax protein, leading to abrogate Tax transactivation of HTLV-1 LTR.

      SUBCELLULAR LOCATION: Nucleus. Note=Associates with centromeric constitutive heterochromatin.

      DEVELOPMENTAL STAGE: Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.

      DOMAIN: SwissProt: O43463 Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.

      PTM: Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.


      SIMILARITY: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. & Contains 1 chromo domain. & Contains 1 post-SET domain. & Contains 1 pre-SET domain. & Contains 1 SET domain.
      Molecular Weight48kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot in U20S cells lysed in RIPA with 0.1% SDS and sonicated.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SUV39H1 (rabbit polyclonal IgG) - 2113358 2113358
      Anti-SUV39H1 (rabbit polyclonal IgG) - 2390481 2390481
      Anti-SUV39H1 (rabbit polyclonal IgG) - 2391009 2391009
      Anti-SUV39H1 (rabbit polyclonal IgG) - 1962108 1962108
      Anti-SUV39H1 (rabbit polyclonal IgG) - 2202526 2202526
      Anti-SUV39H1 - 2032853 2032853
      Anti-SUV39H1 - 26798 26798
      Anti-SUV39H1 - DAM1703510 DAM1703510
      Anti-SUV39H1 - DAM1745362 DAM1745362
      Anti-SUV39H1 - JBC1870073 JBC1870073

      References

      Reference overviewApplicationPub Med ID
      The histone variant composition of centromeres is controlled by the pericentric heterochromatin state during the cell cycle.
      Boyarchuk, E; Filipescu, D; Vassias, I; Cantaloube, S; Almouzni, G
      Journal of cell science  127  3347-59  2014

      Show Abstract
      24906798 24906798
      The SRA protein UHRF1 promotes epigenetic crosstalks and is involved in prostate cancer progression.
      Babbio, F, et al.
      Oncogene, (2012)  2012

      Show Abstract
      22330138 22330138
      SPOC1 modulates DNA repair by regulating key determinants of chromatin compaction and DNA damage response.
      Mund, A; Schubert, T; Staege, H; Kinkley, S; Reumann, K; Kriegs, M; Fritsch, L; Battisti, V; Ait-Si-Ali, S; Hoffbeck, AS; Soutoglou, E; Will, H
      Nucleic acids research  40  11363-79  2012

      Show Abstract
      23034801 23034801
      The histone methyltransferase Set7/9 promotes myoblast differentiation and myofibril assembly.
      Tao, Y; Neppl, RL; Huang, ZP; Chen, J; Tang, RH; Cao, R; Zhang, Y; Jin, SW; Wang, DZ
      The Journal of cell biology  194  551-65  2011

      Show Abstract
      21859860 21859860
      The core binding factor CBF negatively regulates skeletal muscle terminal differentiation.
      Philipot, O; Joliot, V; Ait-Mohamed, O; Pellentz, C; Robin, P; Fritsch, L; Ait-Si-Ali, S
      PloS one  5  e9425  2010

      Show Abstract
      20195544 20195544
      Histone methylation by NUE, a novel nuclear effector of the intracellular pathogen Chlamydia trachomatis.
      Pennini, ME; Perrinet, S; Dautry-Varsat, A; Subtil, A
      PLoS pathogens  6  e1000995  2010

      Show Abstract
      Western Blotting20657819 20657819
      Differential cooperation between heterochromatin protein HP1 isoforms and MyoD in myoblasts.
      Yahi, H; Fritsch, L; Philipot, O; Guasconi, V; Souidi, M; Robin, P; Polesskaya, A; Losson, R; Harel-Bellan, A; Ait-Si-Ali, S
      The Journal of biological chemistry  283  23692-700  2008

      Show Abstract Full Text Article
      18599480 18599480
      Pharmacologic disruption of Polycomb-repressive complex 2-mediated gene repression selectively induces apoptosis in cancer cells.
      Tan, J; Yang, X; Zhuang, L; Jiang, X; Chen, W; Lee, PL; Karuturi, RK; Tan, PB; Liu, ET; Yu, Q
      Genes & development  21  1050-63  2007

      Show Abstract Full Text Article
      Western Blotting17437993 17437993
      Epigenetic regulation of telomere length in mammalian cells by the Suv39h1 and Suv39h2 histone methyltransferases.
      García-Cao, Marta, et al.
      Nat. Genet., 36: 94-9 (2004)  2004

      Show Abstract
      14702045 14702045
      Rb targets histone H3 methylation and HP1 to promoters.
      Nielsen, S J, et al.
      Nature, 412: 561-5 (2001)  2001

      Show Abstract
      Immunoblotting (Western)11484059 11484059

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      05-1248 Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306 Show Pricing & Availability
      05-1248-S Anti-monomethyl Histone H3 (Lys9) Antibody, clone CMA306, Trial Size Show Pricing & Availability
      05-615 Anti-SUV39H1 Antibody, clone MG44 Show Pricing & Availability
      07-958 Anti-SUV39H1 Antibody Show Pricing & Availability
      AB3353 Anti-SUV39H1 Antibody Show Pricing & Availability
      MABE552 Anti-SUV39H1 Antibody, clone EPR4960(2), rabbit monoclonal Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies