05-615 | Anti-SUV39H1 Antibody, clone MG44

05-615
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WB, ChIPMAscitesMonoclonal Antibody
      Description
      Catalogue Number05-615
      ReplacesMABE552
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-SUV39H1 Antibody, clone MG44
      Alternate Names
      • KMT1A
      Background InformationPost -translational modification of histones, such as phosphorylation, acetylation, and methylation, are crucial for chromatin remodeling. SUV39H1 and Suv39h1 are the human and mouse homologs of Drosophila Su(var)3-9 protein, respectively. These proteins selectively methylate histone H3 at lysine 9 creating a high-affinity binding site for the HP1 proteins (heterochromatin protein 1). HP1 proteins are known to interact with transcription suppressor proteins, suggesting that the interactions with SUV39H1 and methylated histone H3 mediate a silence effect on the transcription of target genes. Furthermore, over-expression of SUV39H1 in HeLa cells causes a redistribution of endogenous HP1 proteins and growth retardation, suggesting that SUV39H1-mediated modulation of heterochromatin can impair cell cycle progression.
      References
      Product Information
      FormatAscites
      Control
      • U2OS cell lysate
      PresentationProtein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-SUV39H1 Antibody, clone MG44 is a Mouse Monoclonal Antibody for detection of SUV39H1 also known as KMT1A, Histone H3-K9 Methyltransferase 1 & has been validated in ChIP, IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Biological Information
      ImmunogenMBP fusion protein corresponding to residues 1-412 of human SUV39H1
      CloneMG44
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes SUV39H1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the suppressor of variegation 3-9 homolog family and encodes a protein with a chromodomain and a C-terminal SET domain. This nuclear protein moves to the centromeres during mitosis and functions as a histone methyltransferase, methylating Lys-9 of histone H3. Overall, it plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression.
      Gene Symbol
      • SUV39H1
      • Su
      • SUV39H
      • MG44
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O43463 # Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys- 9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiaton, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length.


      SIZE: 412 amino acids; 47907 Da

      SUBUNIT: Interacts with H3 and H4 histones. Interacts with GFI1B, DNMT3B, CBX1, CBX4, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1. Interacts with SBF1 through the SET domain. Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. In case of infection, interacts with HTLV-1 Tax protein, leading to abrogate Tax transactivation of HTLV-1 LTR.

      SUBCELLULAR LOCATION: Nucleus. Note=Associates with centromeric constitutive heterochromatin.

      DEVELOPMENTAL STAGE: Accumulates during mitosis at centromeres during prometaphase, but dissociates from the centromere at the meta- to anaphase transition.

      DOMAIN: SwissProt: O43463 Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity. The SET domain also participates to stable binding to heterochromatin.

      PTM: Phosphorylated on serine residues, and to a lesser degree, on threonine residues. The phosphorylated form is stabilized by SBF1 and is less active in its transcriptional repressor function.


      SIMILARITY: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. & Contains 1 chromo domain. & Contains 1 post-SET domain. & Contains 1 pre-SET domain. & Contains 1 SET domain.
      Molecular Weight50 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on U2OS cells lysed in RIPA with 0.1% SDS and sonicated
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-SUV39H1, clone MG44 (mouse ascites) - 2005583 2005583
      Anti-SUV39H1, clone MG44 (mouse ascites) - 2199771 2199771
      Anti-SUV39H1, clone MG44 (mouse ascites) - 2332538 2332538
      Anti-SUV39H1, clone MG44 - 20420 20420
      Anti-SUV39H1, clone MG44 - 2073802 2073802
      Anti-SUV39H1, clone MG44 - 2517874 2517874
      Anti-SUV39H1, clone MG44 - DAM1606747 DAM1606747
      Anti-SUV39H1, clone MG44 - DAM1692607 DAM1692607
      Anti-SUV39H1, clone MG44 - DAM1774681 DAM1774681
      Anti-SUV39H1, clone MG44_2842488 2842488

      References

      Reference overviewApplicationPub Med ID
      Suv39h1 mediates AP-2α-dependent inhibition of C/EBPα expression during adipogenesis.
      Zhang, ZC; Liu, Y; Li, SF; Guo, L; Zhao, Y; Qian, SW; Wen, B; Tang, QQ; Li, X
      Molecular and cellular biology  34  2330-8  2014

      Show Abstract
      Western Blotting24732798 24732798
      5-Aza-2'-deoxycytidine reactivates gene expression via degradation of pRb pocket proteins.
      Zheng, Z; Li, L; Liu, X; Wang, D; Tu, B; Wang, L; Wang, H; Zhu, WG
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  26  449-59  2012

      Show Abstract
      21990374 21990374
      p53-mediated heterochromatin reorganization regulates its cell fate decisions.
      Mungamuri, SK; Benson, EK; Wang, S; Gu, W; Lee, SW; Aaronson, SA
      Nature structural & molecular biology  19  478-84, S1  2012

      Show Abstract
      Western Blotting22466965 22466965
      SPOC1 modulates DNA repair by regulating key determinants of chromatin compaction and DNA damage response.
      Mund, A; Schubert, T; Staege, H; Kinkley, S; Reumann, K; Kriegs, M; Fritsch, L; Battisti, V; Ait-Si-Ali, S; Hoffbeck, AS; Soutoglou, E; Will, H
      Nucleic acids research  40  11363-79  2012

      Show Abstract
      23034801 23034801
      Enhanced levels of microRNA-125b in vascular smooth muscle cells of diabetic db/db mice lead to increased inflammatory gene expression by targeting the histone methyltransferase Suv39h1.
      Villeneuve, LM; Kato, M; Reddy, MA; Wang, M; Lanting, L; Natarajan, R
      Diabetes  59  2904-15  2010

      Show Abstract Full Text Article
      20699419 20699419
      Epidermal growth factor receptor regulates beta-catenin location, stability, and transcriptional activity in oral cancer.
      Lee, CH; Hung, HW; Hung, PH; Shieh, YS
      Molecular cancer  9  64  2010

      Show Abstract
      20302655 20302655
      Adenomatous polyposis coli and hypoxia-inducible factor-1{alpha} have an antagonistic connection.
      Newton, IP; Kenneth, NS; Appleton, PL; Näthke, I; Rocha, S
      Molecular biology of the cell  21  3630-8  2010

      Show Abstract Full Text Article
      20844082 20844082
      ASXL1 represses retinoic acid receptor-mediated transcription through associating with HP1 and LSD1.
      Lee SW, Cho YS, Na JM, Park UH, Kang M, Kim EJ, Um SJ
      The Journal of biological chemistry  285  18-29  2010

      Show Abstract Full Text Article
      19880879 19880879
      Hyperglycemia induces a dynamic cooperativity of histone methylase and demethylase enzymes associated with gene-activating epigenetic marks that coexist on the lysine tail.
      Brasacchio, D; Okabe, J; Tikellis, C; Balcerczyk, A; George, P; Baker, EK; Calkin, AC; Brownlee, M; Cooper, ME; El-Osta, A
      Diabetes  58  1229-36  2009

      Show Abstract
      19208907 19208907
      Histone deacetylase inhibitor depsipeptide activates silenced genes through decreasing both CpG and H3K9 methylation on the promoter.
      Wu, LP; Wang, X; Li, L; Zhao, Y; Lu, S; Yu, Y; Zhou, W; Liu, X; Yang, J; Zheng, Z; Zhang, H; Feng, J; Yang, Y; Wang, H; Zhu, WG
      Molecular and cellular biology  28  3219-35  2008

      Show Abstract Full Text Article
      18332107 18332107

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies