05-1044 | Anti-Sin1 Antibody, clone 1C7.2

05-1044
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IP, IH(P), IFMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1044
      DescriptionAnti-Sin1 Antibody, clone 1C7.2
      Alternate Names
      • MEKK2-interacting protein 1
      • Mitogen-activated protein kinase 2-associated protein 1
      • SAPK-interacting protein 1
      • Stress-activated map kinase-interacting protein 1
      • TORC2 subunit MAPKAP1
      • mitogen-activated protein kinase associated protein 1
      • ras inhibitor MGC2745
      • stress-activated map kinase interacting protein 1
      • stress-activated protein kinase-interacting 1
      Background InformationSin1 (stress-activated protein kinase (SAPK)-interacting protein 1, MAPKAP1) is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase along with Sin1, GL (mLST8), Protor1, Protor2, and Rictor. The complex that is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Sin1 is also known to directly interact without proteins that include Ras, SPAKs, and JNK.
      Sin1 is known to have multiple alternative splicing isoforms. Full-length Sin1 is a 522aa protein (59kDa). Sin1 (Isoform 5) (36 kDa) has exon 6 spliced to an alternative exon 7a that results in the loss of both the RBD and PHL domains. Sin1Isoform 2) (55 kDa) lacks exon 7 and lacks the RBD. Sin1 (Isoform 3) (53 kDa) lacks exon 8 lacks the PHL domain. Sin1 (Isoform 4) (37 kDa) lacks exon 1.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal in 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% NaN3.
      Applications
      ApplicationDetect Sin1 using this Anti-Sin1 Antibody, clone 1C7.2 validated for use in WB, IP, IH(P) & IF.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      Biological Information
      ImmunogenFull-length Human Sin1 GST-fusion protein.
      Clone1C7.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityDetects Sin-1 and its isoforms.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, and Rat. Other species have not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. [provided by RefSeq]
      Gene Symbol
      • JC310
      • MGC2745
      • MIP1
      • SIN1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFunction: Essential component of the TORC2 complex, which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Ref.2 Ref.9 Ref.12 Ref.13 Ref.14

      Subunit structure: All isoforms except isoform 4 are able to be part of the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2. Ref.2 Ref.12 Ref.14

      Subcellular location: Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus. Ref.12 Ref.14

      Tissue specificity: Ubiquitously expressed, with highest levels in heart and skeletal muscle. Ref.2

      Sequence similarities: Belongs to the SIN1 family.

      Molecular WeightSin1 is 59 kDa. Isoforms are 36, 37, 41, 53, 54, and 55 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis:
      This lot detected Sin1 at 1:1,000 dilution in A431 cell lysate resolved via SDS-PAGE and transferred to PVDF.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Sin1, clone 1C7.2 2476962
      Anti-Sin1, clone 1C7.2 - 2020882 2020882
      Anti-Sin1, clone 1C7.2 - 2051367 2051367
      Anti-Sin1, clone 1C7.2 - 2202448 2202448
      Anti-Sin1, clone 1C7.2 - 2332537 2332537
      Anti-Sin1, clone 1C7.2 - 2527457 2527457
      Anti-Sin1, clone 1C7.2 - JBC1793814 JBC1793814
      Anti-Sin1, clone 1C7.2 - NG1556538 NG1556538
      Anti-Sin1, clone 1C7.2 - NG1666209 NG1666209
      Anti-Sin1, clone 1C7.2 - NG1692769 NG1692769

      References

      Reference overviewApplicationPub Med ID
      MicroRNA-7 regulates the mTOR pathway and proliferation in adult pancreatic β-cells.
      Wang, Y; Liu, J; Liu, C; Naji, A; Stoffers, DA
      Diabetes  62  887-95  2013

      Show Abstract
      Western Blotting23223022 23223022
      PPIP5K1 modulates ligand competition between diphosphoinositol polyphosphates and PtdIns(3,4,5)P3 for polyphosphoinositide-binding domains.
      Gokhale, NA; Zaremba, A; Janoshazi, AK; Weaver, JD; Shears, SB
      The Biochemical journal  453  413-26  2013

      Show Abstract
      Western Blotting23682967 23682967
      Isolation of the mTOR complexes by affinity purification.
      Dos D Sarbassov,Olga Bulgakova,Rakhmet I Bersimbaev,Tattym Shaiken
      Methods in molecular biology (Clifton, N.J.)  821  2012

      Show Abstract
      22125060 22125060